[PDF] Top 20 Genetic diversity of human immunodeficiency virus type 2: evidence for distinct sequence subtypes with differences in virus biology.

... Nonpathogenic lentiviruses simian immunodeficiency viruses [SIVs] related to HIV-1 and HIV-2 have been identified in different Old World primate species, and phylogenetic analyses of the[r] ... See full document

... global diversity is higher than that of HIV-1. To identify differences in mu- tant diversity, we compared the HIV-1 protease and HCV NS3 protease ...per virus, isolated from a total of six ... See full document

... molecular evidence indicates that subtype K has existed for as long as the other subtypes (24), but its observed worldwide prevalence is ...The genetic distance be- tween the two subtype K sequences ... See full document

... high sequence diversity between HIV-1 and HIV-2, the frequency of the GFP ⫹ phenotype would not correctly reflect the recom- bination ...cleotide sequence identity between the HIV-1 and ... See full document

... no evidence of hypermutation to A was seen in this sequence ...convincing evidence that any specific variant or motif is related to progression of disease or to protection from such progres- ...the ... See full document

... The differences in positive selection between HIV-1 and SIVsmm Gag were particularly strik- ing: there are sites scattered throughout HIV-1 Gag that show recurrent selection throughout the phylogenetic tree ...no ... See full document

... highly type-specific antibody prepara- tions, such as autologous sera or MAbs against the variable loops on gp120 (2, 23, 36, ...tologous virus but no other viruses against which it was ... See full document

... 10 distinct subtypes or clades termed A through J ...acid sequence of the Env protein, whereas variation ranges from 5 to 20% within a ...subtype. Subtypes are not stable entities because ... See full document

... of genetic subtypes recovered from some of the locales studied has also been ...three subtypes were recov- ered from Uganda, and isolates from Zambia included sub- type C and, potentially, an ... See full document

... into sequence subtypes (3, 4, ...same sequence subtype have faster electrophoretic mobilities than heterodu- plexes formed between members of different sequence sub- types, thus forming the ... See full document

... extensive sequence diversity suggest that they might undergo only minor structural constraints and that they have evolved to maximize their possibility of sequence diversification while preserving ... See full document

... at a concentration of 200 ␮M, 15 pmol of each of the primers, and 1 U of Super Taq DNA polymerase (HT Biotechnology, Cambridge, England). A close exam- ination of the sequences available in the HIV sequence ... See full document

... Molecular Biology Laboratory of CIRCB-Yaoundé for analysis at CIRCB-Yaoundé where the samples were stored at − 20 ...3130 genetic analyzer (Applied Biosystems, Foster City, CA) ac- cording to previously ... See full document

... lanes 2 and 3); this result is consistent with the existence of a single functional k B site within the HIV-1 E ...simian immunodeficiency virus isolates obtained from natu- rally infected African ... See full document

... 3602 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest In contrast to infrequent and low-titer cross-neutralization of human immunodeficiency virus type 1 HIV-1 isolates b[r] ... See full document

... for virus trans- mission, a cell line which inducibly expresses DC-SIGN was ...efficient virus transmission, cells were seeded in duplicate wells and DC-SIGN expression was induced by addition of ...the ... See full document

... 12 0022-538X/92/127355-07$02.00/0 Copyright © 1992, American Society for Microbiology Evidence that a Sequence Similar to TAR Is Important for Induction of the JC Virus Late Promoter by [r] ... See full document

... HIV-2 genetic diversity, we first tried to select conserved and efficient segments of the viral genome to use as ...A subtypes but failed to amplify any B ...(1, 2, 31). In contrast, ... See full document

... revertants. Virus supernatant was harvested at the peak of virus replication from CEM (12D-7) or H9 cultures infected with mutant d5, d7, or ...d8. Virus stocks were normalized for reverse ... See full document

... Restriction maps were constructed for the proviral DNAs present in A3.01 cells infected with plaque-purified viral recombinants derived from pNLtat tat-01, tat-02, tat-03, tat-04, and ta[r] ... See full document