[PDF] Top 20 Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
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Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... study, PCR amplification of the coa gene revealed significant differences between ...S. aureus isolates from pastry and cheese ...S. aureus isolates may be negative for the coa ...S. ... See full document
6
Molecular Diversity of Methicillin-resistant Staphylococcus aureus Isolates Originated from Patients in Ahvaz Hospitals, Iran
... Introduction: Staphylococcus aureus is among the primary cause of hospitals and community-acquired ...S. aureus (MRSA) strains has resulted in the treatment failure of the infections caused by these ... See full document
10
Comparison of Pulsed Field Gel Electrophoresis and Coagulase Gene Restriction Profile Analysis Techniques in the Molecular Typing of Staphylococcus aureus
... contrast, PCR-based CRP analysis is fast and easy to ...CRP analysis ex- hibits the same discriminatory power as PFGE for SEA-E- producing ...CRP analysis is much less dis- criminatory than ... See full document
5
Molecular Typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene
... used PCR RFLP pattern analysis to study S. aureus, but only Tenover et ...differing PCR primers were employed (15, 25, 26, 29, 38, ...previous coagulase PCR RFLP ... See full document
7
Comparison of Susceptibility Testing Methods withmecA Gene Analysis for Determining Oxacillin (Methicillin) Resistance in Clinical Isolates of Staphylococcus aureus and Coagulase Negative Staphylococcus spp
... mecA gene by using a modification of a previously described multiplex PCR method ...following PCR primers were used for amplification of the mecA gene: mec449F, 5⬘-AAA CTA CGG TAA CAT ... See full document
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Coagulase gene polymorphism, enterotoxigenecity, biofilm production, and antibiotic resistance in Staphylococcus aureus isolated from bovine raw milk in North West India
... S. aureus contami- nation was present in 73 ...plating analysis. All 73 isolates were found as S. aureus in API ...showed coagulase activity and were further confirmed as S. aureus by ... See full document
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Clonal analysis and identification of epidemic strains of methicillin resistant Staphylococcus aureus by antibiotyping and determination of protein A gene and coagulase gene polymorphisms
... (iii) PCR amplification and restriction analysis of the staphylococcal coagu- lase ...the coagulase gene was amplified with two primers: COAG 2 (5 9 CGAGACCA AGATTCAACAAG 3 9 ) and COAG 3 (5 9 ... See full document
7
Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene.
... S. aureus, analysis of the isolates by discriminatory DNA typing methods, and understanding the transmission of the bacterial infection ...methicillin-resistant Staphylococcus aureus (MRSA) ... See full document
7
Molecular analysis of Coagulase (coa) gene polymorphism in clinical isolates of Staphylococcus aureus by PCR-RFLP
... Staphylococcus aureus is one of the major pathogens which cause different diseases in human ...The analysis of various virulence factors responsible for the pathogenecity of ...of coagulase ... See full document
6
Dissemination of Two Methicillin Resistant Staphylococcus aureus Clones Exhibiting Negative Staphylase Reactions in Intensive Care Units
... RAPD analysis generated by AP-PCR has been documented to be a powerful molecular method for the typing of a wide variety of bacteria ...(7–9). RFLP analysis of the coagulase gene ... See full document
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Typing of HVR, Frequency of blaZ, and Detection of mecA Promoter Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
... resistant Staphylococcus aureus strains in burn patients, Tehran, ...Methicillin-resistant Staphylococcus aureus (MRSA) ...S. Analysis of diversity of muta- tions in the mecI ... See full document
6
Spread of Influenza A(H1N1) oseltamivir resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal
... The present study provides genetic evidence of circulation of drug-resistant seasonal A(H1N1) viruses in Senegal du- ring the 2008 influenza season (July to September). It shows that RT-PCR-RFLP method, a ... See full document
7
Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates
... Sensitive Staphylococcus aureus Isolates in Tehran, ...resistant Staphylococcus aureus (MRSA) strains isolated from nose of personnel in Hajar hospital of Shahre-kord, ... See full document
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Studies of Staphylococcal Infections I Virulence of Staphylococci and Characteristics of Infections in Embryonated Eggs
... GROWTH CURVES AND CUMULATIVE FATALITY crease in the number of coagulase negative staphy- RATES OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCUS lococci was observed in both the embryo and EPI[r] ... See full document
13
Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects
... There was no significant difference between smokers and disease, suggesting similar bacterial community composition and diversity (p = 0.08, Student’s t-test). The diversity index (Table 2) was significantly lower in ... See full document
17
Differentiation of Borrelia burgdorferiSensu Lato on the Basis of RNA Polymerase Gene (rpoB) Sequences
... In the phylogenetic tree based on the rpoB sequences, B. burgdorferi sensu lato could be distinguished from B. turica- tae and B. hermsii, which are associated with relapsing fever. Each species of B. burgdorferi sensu ... See full document
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ANALYSIS OF POLYMORPHISM OF CAST GENE AND CLPG GENE IN SHEEP BY PCR-RFLP METHOD
... In this study were collected blood samples from 96 sheep (rams and ewes) kept in Slovakia. This population involved 60 sheep of breed tsigai, 19 sheep of breed improved valachian, 2 sheep of breed east friesian, 11 sheep ... See full document
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CAPSULAR TYPING OF COAGULASE POSITIVE (COPS) COMMUNITY ASSOCIATED METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (CA-MRSA) ISOLATED FROM ANTERIOR NARES OF SCHOOL CHILDREN FROM LUSHOTO, KOROGWE, MUHEZA AND TANGA DISTRICTS IN TANZANIA
... significant Staphylococcus aureus type 5 CP (CP 5) and type 8 CP (CP 8) are comprised of a trisaccharide repeat unit with one O-acetyl group attached to each repeat ... See full document
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Assessment of the Coagulase Test in the Identification of Staphylococcus Aureus Strains
... Staphylococcus aureus are the most common ...identifying Staphylococcus aureus strains in the ...the coagulase test for the identification of Staphylococcus aureus ...the ... See full document
7
A Preliminary Study on the Molecular Identification of Herpes Simplex Virus Type 1 in Iranian Population
... Also, a similar molecular epidemiological technique, by which the vaccine strain of VZV can easily be distinguished from the circulation wild-type strains, was developed to accurately evaluate the protective efficacy of ... See full document
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