[PDF] Top 20 Hepatitis C Virus Genotyping Using an Oligonucleotide Microarray Based on the NS5B Sequence
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Hepatitis C Virus Genotyping Using an Oligonucleotide Microarray Based on the NS5B Sequence
... HCV genotyping (50), including commercially available techniques based on real-time PCR: the HCV genotyping analyte-specific reagent (ASR) assay (Abbott Molecular ...HCV genotyping methods are ... See full document
8
Hepatitis C virus genotyping based on Core and NS5B regions in Cameroonian patients
... was based on core region only (Table ...the NS5B was identified as genotype 1 indi- cating the presence of putative HCV recombinant viruses or multiple infections in these samples (Table ... See full document
7
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
... Hepatitis C virus genotyping assays are usually based on the sequence analysis of an amplified segment of the genome, which is commonly the 5’ untranslated ...5’UTR based ... See full document
5
Genotyping of Measles Virus in Clinical Specimens on the Basis of Oligonucleotide Microarray Hybridization Patterns
... Health using microarray slides made at the FDA ...of genotyping were 100% concordant with the genotypes previously identified by sequence ...The microarray detected MV in 14 samples ... See full document
8
Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA – effects on NS5B catalysis and allosteric inhibition
... RNA sequence used was originally con- structed for a fluorescent based de novo polymerase activity assay ...poly(G) sequence, this is a likely explanation for its binding to ...consensus ... See full document
11
Rapid Genotyping of Hepatitis C Virus by Primer Specific Extension Analysis
... accurate genotyping of hepatitis C virus (HCV) is becoming increasingly important for clinical management of chronic infection and as an epidemiological ...most genotyping methods. We ... See full document
6
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... complete NS5B gene and included 54 nucleotides of the 3 9 nontranslated ...region. NS5B proteins with six histidine residues at their amino termini were translated from plasmid ...complete NS5B ... See full document
13
Sequence-based genotyping of hepatitis B virus in general popula-tion
... types have largely been conducted among patients with chronic hepatitis B (CHB) from Iran (26). However, limited studies carried out to determine genotypes of this virus in Iranian general population (24). ... See full document
7
Sequencing Assays for Failed Genotyping with the Versant Hepatitis C Virus Genotype Assay (LiPA), Version 2 0
... high sequence diversity and HCV strains are classified into genetically distinct groups, which are known as genotypes when differences at the nucleotide level range from 31% to 33% or as subtypes when differences ... See full document
7
Detection and Genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by Oligonucleotide Microarray
... target sequence with a pair of specific or degenerate primers is often more efficient and convenient than multiplex or random amplifica- tion, but there are two salient ...that sequence variability among ... See full document
10
Simplified Hepatitis C Virus Genotyping by Heteroduplex Mobility Analysis
... for genotyping HCV is nucleotide sequencing of NS5b ampli- ...(8). Genotyping by HMA involves mixing a PCR product of unknown genotype separately with a panel of reference prod- ucts of each genotype ... See full document
6
Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization
... rotaviruses based on oligonucleotide microarray hybridization has been ...was based on hybridization with several individual genotype-specific ...of oligonucleotide microchip ... See full document
10
Microarray for Hepatitis B Virus Genotyping and Detection of 994 Mutations along the Genome
... of hepatitis B virus (HBV) in patient sera is helpful for monitoring ...DNA microarray to identify HBV genotypes and to detect mutations of interest in the S, Pol, Core, and X ...allowed ... See full document
9
Evaluation of the Invader Assay for Genotyping Hepatitis C Virus
... HCV NS5B sequencing by the TRUGENE HCV NS5B Genotyping Assay was attempted with this specimen but failed to generate a ...region sequence of this clone was consistent with that of genotype 3b, ... See full document
6
Microarrays for Genotyping Human Group A Rotavirus by Multiplex Capture and Type Specific Primer Extension
... with sequence-specific hybridization probes (GeneChips) synthesized in situ was pro- posed for large-scale analysis of the genetic variation of human immunodeficiency virus (HIV) 6 years ago ...of ... See full document
6
cis-Acting RNA Signals in the NS5B C-Terminal Coding Sequence of the Hepatitis C Virus Genome
... HCV NS5B belongs to the large family of nucleic acid poly- merases that possess the structure of a right hand consisting of finger, thumb, and palm domains (6, ...HCV NS5B has been expressed and purified ... See full document
13
Molecular Evolution Studies on Hepatitis C Virus based on NS5B Region
... However Kapoor et al. [2] reported that a single stranded RNA virus which belongs to genus Hepacivirus infects the very close friend of humans “the dogs” and causes pulmonary infection in dogs. These viruses are ... See full document
6
Use of Sequence Analysis of the NS5B Region for Routine Genotyping of Hepatitis C Virus with Reference to C/E1 and 5′ Untranslated Region Sequences
... gene sequence was determined for three of ...UTR, C, and E1 se- quences are available ...93% sequence homology to Z1 in C/E1. QC264 in NS5B did not group with previously identified ge- ... See full document
11
Comparison of Reverse Hybridization, Microarray, and Sequence Analysis for Genotyping Hepatitis B Virus
... the amplicon was sequenced with the BigDye Terminator version 3.1 cycle sequencing kit (Applied Biosystems, Nieuwerkerk a/d IJssel, The Netherlands). The PCR products were precipitated with 80 l NaAc buffer (3 l 3 M ... See full document
6
Analysis of the 5′ Noncoding Region versus the NS5b Region in Genotyping Hepatitis C Virus Isolates from Blood Donors in France
... the hepatitis C virus (HCV) has become the standard for genotyping even though several reports show that its use can result in classification ...perform genotyping based on ... See full document
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