[PDF] Top 20 Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... B capsids. To con- firm that UL25 was present in substantially reduced amounts in the UL17-stop mutant capsids, Western blot analysis was carried ...UL6, UL17, UL25, and VP23 ... See full document
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The Herpes Simplex Virus Type 1 DNA Packaging Protein UL17 Is a Virion Protein That Is Present in Both the Capsid and the Tegument Compartments
... infectious virus. In the absence of DNA packaging, the internal scaffolding proteins are still cleaved but remain trapped inside the angularized capsid, generating a dead-end product, the B ...A ... See full document
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The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... The UL25 protein is associated with purified ...the UL25 protein is important for retaining cleaved DNA in capsids suggests that this protein may interact with ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 UL25 DNA Packaging Protein Reveals Regions That Are Important after the Viral DNA Has Been Packaged
... wt virus, with enveloped virus particles readily detected in the cytoplasm, often in vesicles, and on the cell ...the virus genome. Recently, a UL25 ts mutant, ts1249, was shown to have an ... See full document
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Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... mutant virus with respect to the nuclear egress of nucleocapsids is, to some extent, similar to that of wild-type HSV-1(F) infection of nucleolin-depleted cells, nucleolin might interact with UL12 to ... See full document
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The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores
... cGAS DNA-sensing cascade is severely dimin- ished in vFH518 and vFH589 ...genomic DNA becomes exposed to cellular pattern recognition receptors ...For DNA viruses, activation of PRRs contributes to ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... DNA packaging is a critical step in the lytic replicative cycle of herpes simplex virus type 1 ...the packaging reaction, replicated DNA is resolved from ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... assembled capsids were stained with monoclo- nal antibody 5C, which recognizes hexons within preassembled ...which DNA synthesis occurs ...which capsids are distrib- uted throughout the nucleus, with ... See full document
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Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
... HSV-1 capsids and packaging DNA is similar to that of double-stranded DNA (dsDNA) bacteriophages such as T4, P22, and lambda (9, 27, 28, ...in UL25 null mutant- infected cells ... See full document
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... the DNA strand was reported for the T4 gene 32 protein ...the protein chain, around which the ssDNA is presumably wrapped, has been shown by crystallography ...replication protein A, the ... See full document
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Herpes Simplex Virus Type 1 Cleavage and Packaging Proteins UL15 and UL28 Are Associated with B but Not C Capsids during Packaging
... and packaging proteins in procapsids and ma- ture virions may provide clues as to their possible ...and UL25, two of the HSV-1 DNA cleavage and pack- aging proteins, are found in ... See full document
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Herpes Simplex Virus Capsid Structure: DNA Packaging Protein UL25 Is Located on the External Surface of the Capsid near the Vertices
... of UL25 from insect cells was carried out beginning with Sf9 cells infected with ...A capsids plus 125 l TNE and incubated for 1 h at ...incubation, capsids were harvested by pelleting ... See full document
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Herpes Simplex Virus DNA Cleavage and Packaging: Association of Multiple Forms of UL15-Encoded Proteins with B Capsids Requires at Least the UL6, UL17, and UL28 Genes
... of capsids, designated A, B, and C, that differ in density and electron microscopic appearance accumu- late in the nuclei of cells infected with wild-type herpes simplex virus ... See full document
6
The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids
... (lanes 1, 4, and 7) in the presence (lanes 1 and 2) or the absence (lanes 3 to 7) of ...UL42 protein (an early protein not incorporated into virions) to assay for de novo protein ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... herpesvirus DNA is cleaved and packaged but is then either lost from capsids or retained in capsids that do not protect the DNA from digestion with ...the packaging signal can be ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... A capsids and DNase I-sensitive unit-length genomes ac- cumulated in the nuclei of cells infected with KUL25NS, sug- gesting that, although complete genomes could be packaged, the viral DNA was only ... See full document
9
Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
... The herpes simplex virus 1 (HSV-1) virion consists of a 152-kbp double-stranded DNA (dsDNA) genome that is en- closed in an icosahedral capsid, which is itself surrounded by an ... See full document
13
Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... single-stranded DNA are required for efficient enzyme ...that binding of UL5-UL52 to DNA requires at least 12 nt of ssDNA if the entire DNA is single stranded ...of DNA ... See full document
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The Herpes Simplex Virus 1 UL17 Protein Is the Second Constituent of the Capsid Vertex-Specific Component Required for DNA Packaging and Retention
... the DNA-terminase complex binds and together function as an ATP-driven motor to pump the viral DNA into the capsid and where UL17 may function to support postpack- aging cleavage of the ...nal ... See full document
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The Herpes Simplex Virus Type 1 UL17 Gene Encodes Virion Tegument Proteins That Are Required for Cleavage and Packaging of Viral DNA
... During herpes simplex virus type 1 (HSV-1) infection, indi- vidual viral genomes are cleaved from intranuclear con- catameric viral DNA and packaged into preassembled ... See full document
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