[PDF] Top 20 Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface.
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Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface.
... of herpes simplex virus (HSV) into host cells is thought to involve fusion of the virion envelope with the plasma membrane (reviewed in reference ...of infected cells can ... See full document
8
Plasma Membrane Topology of Syncytial Domains of Herpes Simplex Virus Type 1 Glycoprotein K (gK): the UL20 Protein Enables Cell Surface Localization of gK but Not gK-Mediated Cell-to-Cell Fusion
... to cell surfaces (data not shown), imply- ing that this truncated gK may be substantially misfolded and entrapped in the RER ...membrane fusion phenomena because it con- tains the majority of the ... See full document
12
Multiple Roles of the Cytoplasmic Domain of Herpes Simplex Virus 1 Envelope Glycoprotein D in Infected Cells
... the cell surface in in- fected cells and to promote viral cell-cell spread (9, ...68-infected cells were induced by the cytoplasmic domain of gp48, and MHV-68 virions move ... See full document
12
Mutational Evidence of Internal Fusion Loops in Herpes Simplex Virus Glycoprotein B
... gB cell surface expression, we used a modification of a cell-based enzyme-linked immunoabsorbent assay (CELISA) method (16, ...CHO-K1 cells growing in 96-well plates were transfected with the ... See full document
9
Contribution of Endocytic Motifs in the Cytoplasmic Tail of Herpes Simplex Virus Type 1 Glycoprotein B to Virus Replication and Cell-Cell Fusion
... the cell surface, most of them homogenously surround- ing the nuclei of infected ...the cell surface at 15 and 30 min after the shift ...had yet gained the TGN compart- ment at ... See full document
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The Amino Terminus of Herpes Simplex Virus Type 1 Glycoprotein K (gK) Modulates gB-Mediated Virus-Induced Cell Fusion and Virion Egress
... are known to cause extensive virus-in- duced cell fusion (9, 10, 28, ...extensive cell fusion, while larger deletions, which encompassed the en- tire predicted alpha-helical ... See full document
13
Responses of Herpes Simplex Virus Type 1-Infected Cells to the Presence of Extracellular Antibodies: gE-Dependent Glycoprotein Capping and Enhancement in Cell-to-Cell Spread
... epithelial cells, HSV virions have been sug- gested to be preferentially targeted, via a gE-mediated func- tion, to lateral junctions rather than the apical surface ...Because virus particles are ... See full document
8
Insertion Mutations in Herpes Simplex Virus 1 Glycoprotein H Reduce Cell Surface Expression, Slow the Rate of Cell Fusion, or Abrogate Functions in Cell Fusion and Viral Entry
... of cells transfected with selected mutants were also probed with anti-gH-gL polyclonal antibody by Western blot- ting to determine if gH was translated despite low levels of cell surface ...the ... See full document
9
Mechanism of Neutralization of Herpes Simplex Virus by Antibodies Directed at the Fusion Domain of Glycoprotein B
... As yet, we do not know how FR1 works to direct the FLs and, consequently, to promote the process of membrane ...the virus and im- pair cell-cell fusion? Furthermore, what does ... See full document
13
Mechanism of Neutralization of Herpes Simplex Virus by Antibodies Directed at the Fusion Domain of Glycoprotein B
... As yet, we do not know how FR1 works to direct the FLs and, consequently, to promote the process of membrane ...the virus and im- pair cell-cell fusion? Furthermore, what does ... See full document
13
The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment
... plates. Cell monolayers were transfected with the indi- cated UL20 and/or gK plasmid combinations by using Lipofectamine 2000 (Invitrogen) according to the manu- facturer's instructions and prepared for confocal ... See full document
12
Herpes Simplex Virus 1 Glycoprotein M and the Membrane-Associated Protein UL11 Are Required for Virus-Induced Cell Fusion and Efficient Virus Entry
... Vero cells were transfected with plasmids expressing either gM or UL11 and subsequently infected with the mutant viruses were ...measure cell-to-cell fusion (see Materials and ...higher ... See full document
9
Herpes Simplex Virus Glycoprotein B Associates with Target Membranes via Its Fusion Loops
... proteinaceous cell surface receptor and that fusion loop mutants are unable to function in fusion because they fail to recognize this ...soluble fusion loop mutants should be impaired ... See full document
12
Regulation of Herpes Simplex Virus Glycoprotein-Induced Cascade of Events Governing Cell-Cell Fusion
... detect cell surface expression of the glycoproteins, we used a modified cell-based enzyme-linked immunosorbent assay (CELISA) (51, ...B78H1 cells growing in 96-well plates were trans- fected ... See full document
10
Interaction of herpes simplex virus glycoprotein gC with mammalian cell surface molecules.
... polyhedrosis virus) by using Baculogold (Pharmingen) as the source of baculovirus DNA ...and infected cell supernatants were screened for gC expression by sodium dodecyl sulfate-polyacrylamide gel ... See full document
13
Herpes simplex virus glycoprotein K promotes egress of virus particles.
... the cell surface. One camp suggests that enveloped virus particles are transported to the cell surface in the interior of a series of membranous vesicles or tubules derived from the ER ... See full document
13
Truncation of Herpes Simplex Virus Type 2 Glycoprotein B Increases Its Cell Surface Expression and Activity in Cell-Cell Fusion, but These Properties Are Unrelated
... both cell-cell fusion assays, but not in the mutants with increased activity in one or both ...Epstein-Barr virus and other lymphocryptoviruses, and for some viruses with this motif, ... See full document
13
Virus-specific Ribonucleic Acid Synthesis in KB Cells Infected with Herpes Simplex Virus
... This paper describes studies of the relationship of virus messenger ribonucleic acid mRNA synthesis to virus DNA, antigenic protein, and infectious-particle production in KB cells infect[r] ... See full document
8
Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... temperature-sensitive cell line encoding a mutant HCF, HSV-1 protein synthesis and replication were significantly reduced at the nonpermissive ...temperature-sensitive cell lines (43, 54), and this could be ... See full document
11
Nuclear Pore Composition and Gating in Herpes Simplex Virus-Infected Cells
... Nup358 were reduced in infected cells Fig. 4b, lanes 1 and 2). This relative reduction was again observed in the WGA-en- riched fraction, when probed with the O-GlcNAc antibody (Fig. 4b, lanes 5 and 6). One ... See full document
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