[PDF] Top 20 Herpes simplex virus type 1 ICP8: helix-destabilizing properties.

... The assay was performed in the presence of M13-22-mer substrate, 0.27 ,uM ICP8, and the indicated concentrations of MgCI2 as described in Materials and Methods.. Kinetics of strand displ[r] ... See full document

... assembled into nucleosomes via replication-independent path- ways (77). H3.3 would therefore be the most likely H3 variant available to be assembled in nucleosomes with infecting HSV-1 genomes. Consistently, ... See full document

... Additionally or alternatively, newly replicated genomes whose origin binding sites are occupied by OBPC and not OBP would not serve as templates but rather would be directed to the packaging machinery. Bacteriophage f ... See full document

... The resulting mixture was subjected to centrifugation for 60 min at 100,000 x g, and the supernatant was studied by Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Lysa[r] ... See full document

... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document

... In addition to cell lines which complemented ts mutants, two lines, U-27 and U-35, significantly inhibited plaque formation by wild-type virus, contained 30 and 100 copies of the ICP8 ge[r] ... See full document

... for ICP8 activity, we changed them each individually to alanine residues and assayed their abilities to complement 8lacZ ...of ICP8 complemented 8lacZ replication to an extent similar to that of ... See full document

... 300 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In a previous work, it was demonstrated that the bacterial transposon TnS is capable of undergoing sequence inversio[r] ... See full document

... 6945 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complem[r] ... See full document

... 25 take the view that the accumulation of virus particles at the cell surface by interaction with heparin sulfate is followed by the interaction of the virus with a saturable receptor in[r] ... See full document

... The cells used in this study were baby hamster kidney cells BHK-21; 5-bromo2'-deoxyuridine BUdR-resistant BHK cells BUBHK, which express no cellular TK; and PyY/TG/ 649 Downloaded from h[r] ... See full document

... These small microfoci displayed a high mobility within the nucleus (not shown), reflecting active movement of nuclear capsids (21). The localization of the VP26 and VP16 fusion proteins to RCs was confirmed by staining ... See full document

... delocalization of nucleolin and for the formation of nucleolin granular dots, we analyzed the nucleolin localization by immu- nofluorescence in cells infected with HSV-1 strains defective in specific viral genes. ... See full document

... unique properties of OBP may reflect characteristics of the replication initiation complex specific to HSV and play a role in the patho- genesis of the virus is of considerable ... See full document

... cial properties of the a sequence could be functionally replaced by a DNA sequence and endonuclease which were both bac- terial in ...the properties of both the HSV-1::L-S D a virus in this ... See full document

... Analysis of extracts derived from cells coinfected with V37 and an ICP8-expressing vaccinia virus recombinant V8 and analysis of extracts prepared from cells infected with the HSV-1 ICP8[r] ... See full document

... Although different purification strategies lead to some vari- ation in its protein components, the cellular Pol II holoenzyme is a large, multisubunit complex composed of the core Pol II, general transcription factors ... See full document

... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document

... 615 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The initiation site for transcription of the herpes simplex virus type 1 HSV-1 gene encoding the major DNA-binding p[r] ... See full document

... when virus was incubated with EDTA-treated serum compared with PBS; (ii) HSV enzyme- linked immunosorbent assay (ELISA); and (iii) HSV-1 and -2 Western blot (immunoblot) ..., 1:40; early antigen, ... See full document