[PDF] Top 20 High Throughput Pooling and Real Time PCR Based Strategy for Malaria Detection
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High Throughput Pooling and Real Time PCR Based Strategy for Malaria Detection
... the real-time PCR assay. The sensitivities of PCR-based assays typically dif- fer on the basis of common factors, such as sample quality, real-time system, and cycling ... See full document
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PCR Based Pooling of Dried Blood Spots for Detection of Malaria Parasites: Optimization and Application to a Cohort of Ugandan Children
... RDTs, PCR is well known to provide improved sensitivity and specificity ...turnaround time for PCR makes it impractical for rapid diagnosis of indi- vidual patients, and in surveillance settings, it ... See full document
5
Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
... Using PCR as the signal amplification system, the sen- sitivity of IPCR was significantly enhanced comparing with ELISA ...quantitative real time PCR (QRT-PCR) instruments enables IPCR ... See full document
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A Rapid and High Throughput Screening Approach for Methicillin Resistant Staphylococcus aureus Based on the Combination of Two Different Real Time PCR Assays
... MRSA-positive PCR result in the SMM panel, a second real-time PCR assay (GeneOhm MRSA; BD Diagnostics, San Diego, CA, USA) was performed on the vast majority of ...MRSA detection were ... See full document
7
Modeling of 5′ Nuclease Real Time Responses for Optimization of a High Throughput Enrichment PCR Procedure for Salmonella enterica
... enrichment PCR in order to avoid false-negative results due to PCR inhibition of enrichment media (20), the influences and interactions of various PCR reagents on the detection response were ... See full document
9
Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real Time PCR
... ABSTRACT Malaria is one of the leading causes of infectious disease in travelers returning from the ...of malaria is typically performed by exam- ining Giemsa-stained thick and thin peripheral blood smears, ... See full document
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Comparison of Microscopy, Nested PCR, and Real Time PCR Assays Using High Throughput Screening of Pooled Samples for Diagnosis of Malaria in Asymptomatic Carriers from Areas of Endemicity in Myanmar
... where malaria is ...have high levels of gametocytes and constitute a reservoir available for new ...sample pooling/PCR-based molecular detection strategy for screening ... See full document
8
Effective high throughput blood pooling strategy before DNA extraction for detection of malaria in low transmission settings
... the malaria diagnosis by molecular means, a pool- ing method was proposed by Bharti et ...Similarly, pooling of the genomic DNA before amplification [9,16] was reported in detection of ma- laria in ... See full document
8
Are Molecular Tools Solving the Challenges Posed by Detection of Plant Pathogenic Bacteria and Viruses?
... for detection and diagnosis of these plant pathogens are required to apply treatments, undertake agronomic measures or proceed with eradication practices, particularly for quarantine ...protocols based on ... See full document
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Comparison of real time PCR and microscopy for malaria parasite detection in Malawian pregnant women
... pfldh real-time PCR assay we employed is highly sensitive and specific for ...general, real-time PCR assays offer specific advantages over other PCR-based chemis- ... See full document
9
Comparative evaluation of published real time PCR assays for the detection of malaria following MIQE guidelines
... numerous PCR methods that have been developed for the laboratory diagnosis and clinical management of malaria ...for detection of ...qPCR malaria assays not included, pub- lished or not, that ... See full document
8
Simplified Pan-species Real-time PCR-based Detection of Plasmodium Spp. in Blood Smear
... control malaria over the re- cent decades have been largely successful; however, a limited number of cases have been found in the southern and eastern parts of the country (5, ... See full document
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Monitoring and evaluation of lymphatic filariasis interventions: an improved PCR based pool screening method for high throughput Wuchereria bancrofti detection using dried blood spots
... Wb PCR assays are not currently reported as quantita- tive. We generated a standard curve using 100 μl of lys- ate from blood samples with 1, 3, 10 or 100 mf to be able to quantify the qPCR results (Figure 2). ... See full document
5
Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... Real-time-PCR assay. The oligonucleotide primers and molecular-beacon probe were designed (using Oligo software from Beacon Designer-2.1; Bio-Rad) to specifically amplify a 134-bp fragment inside the ... See full document
5
Real Time PCR Method for Detection of Zygomycetes
... to PCR using the primers described above and targeting a 167-bp region of the cytochrome b ...The PCR product was then ligated into the PCR ...Roche High Pure plasmid isolation kit (Roche ... See full document
7
Developing a real time PCR assay based on multiplex high resolution melt curve analysis: a pilot study in detection and discrimination of soil transmitted helminth and schistosome species
... For Peer Review Developing a real time PCR assay based on multiplex high resolution melt curve analysis A pilot study in detection and discrimination of soil transmitted helminth and schistosome speci[.] ... See full document
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Simple Real Time PCR and Amplicon Sequencing Method for Identification of Plasmodium Species in Human Whole Blood
... rRNA real-time PCR targets and an 18S rRNA sequencing ...demonstrated high specificity for the intended species, with the following ...of time and subjected to multiple freeze-thaw cy- ... See full document
7
Detection and quantification of microRNA in cerebral microdialysate
... a high RR when using a 20 kDa microdi- alysis ...a high RR was confirmed using the same criteria as for the in vitro study (Figure 2 - ...a high RR; only one miRNA showed a markedly different ... See full document
10
PCR Based Rapid Identification System Using Bridged Nucleic Acids for Detection of Clarithromycin Resistant Mycobacterium avium M intracellulare Complex Isolates
... the PCR product that showed clarithromycin resistance was implemented and showed that the clarithromycin-resistant samples, except for eight mutant specimens, were WT ...(-gaAAag-). Based on the this ... See full document
6
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... for detection of bacterial meningitis pathogens are continuously being ...improved. Detection of the specific sero- group or serotype causing disease can lead to better understanding of disease ... See full document
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