[PDF] Top 20 Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a 10 Minute Extraction Protocol
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Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a 10 Minute Extraction Protocol
... Gram-negative bacteria than of Gram-positive bacteria, as previously reported ...Gram-positive bacteria than those in other studies (18, 26) and obtained better results for Streptococcus pneumoniae ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry represents a revolution in the rapid iden- tification of bacterial ... See full document
5
Rapid Identification of Bacteria from Positive Blood Culture Bottles by Use of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Fingerprinting
... Direct identification by mass spectrometry ...recovered from blood culture fluid by stepwise centrifugation (44) and prepared for mass spectrometry ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... 2015 from outpa- tients and inpatients with symptoms suggestive of ...red blood cells, epithelial cells, bacteria, and Can- dida ...that, 10 l of urine was cultured onto chromogenic chromID ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... automated blood culture and 16S sequencing devices, and the marginal costs are substantially less than those of conven- tional identification ...as 10% of the price of the instrument each ... See full document
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... specimens from adult patients were systematically collected and cultured using the BD BACTEC system (BD, Franklin Lakes, NJ, USA), bioMérieux BacT/ALERT system (bioMérieux, Marcy l’Etoile, France), and ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... MS identification protocol (MALDI ...performed using the Bruker Microflex instrument, Biotyper software ...MALDI protocol was developed based on the manufacturer’s suggestions and findings ... See full document
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... for direct analysis in the Bruker MALDI BioTyper (study identification method) using an in- house extraction ...MALDI matrix (a saturated so- lution of ␣ -cyano-4-hydroxycinnamic acid ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... the identification of clinical yeast and yeast-like ...on-plate extraction methods pro- vide a definitive identification for the majority of yeast isolates within 10 to 40 min with only 5 min ... See full document
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Rapid Identification of Bacteria and Yeasts from Positive Blood Culture Bottles by Using a Lysis Filtration Method and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrum Analysis with the SARAMIS Database
... Rapid identification of microorganisms causing bloodstream infections directly from a positive blood culture would decrease the time to directed antimicrobial therapy and greatly ... See full document
5
Evaluation of a Semiquantitative Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
... incubation time, concentration of the substance, and the antibiotic-specific time-kill ...with bacteria extracted from the anaerobic ...with bacteria that were extracted from the ... See full document
5
Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... Molecular identification assays are accu- rate tools for species diagnosis, but precautions are required during several steps from the storage of specimens/samples to PCR amplifi- cation to ensure correct ... See full document
9
Applications of MALDI TOF MS in Clinical Microbiology Laboratory
... microbial identification and strain typing of bacteria, fungi and ...pathogens from direct samples. Bacterial identification: Conventional laboratory techniques for ... See full document
7
Optimization of Routine Identification of Clinically Relevant Gram Negative Bacteria by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and the Bruker Biotyper
... the time it takes to identify these ...full extraction (13, ...of identification but induced no misidentifications, verifying that MALDI-TOF analysis can be performed from primary subcul- ... See full document
9
Evaluation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates
... Identification of the 125 NTM isolates by MALDI-TOF and 16S rRNA/hsp65 sequencing was compared, and agreement be- tween methods at the species level was shown in 118/125 cases (94.4%) (Table 2). A score of ⬎ 1.7 ... See full document
5
Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel
... study using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to determine the identification of ... See full document
7
Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... for mass calibration as per manufacturer’s guidelines and showed a typical Escherichia coli DH5 alpha peptide and protein profile with additional protein peaks (data not ...HCCA matrix, and allowed to dry ... See full document
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... the identification rate using the combined UW/Bruker ...the identification rate increased to ...identified using the combined UW/Bruker database, and ...identified using the Bruker ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... of identification to be determined for ...available identification systems based on phenotypic features, and false identification might often occur with these systems (see Ta- ble S1 in the ... See full document
7
Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... ranged from 52 to 59 carbons, with the main peak belonging to mycolic acid, with a chain length of 55 carbon atoms and 4 double bonds (the observed value of the sodi- ated molecular ion was m/z ...acid ... See full document
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