[PDF] Top 20 Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
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Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... The UL56 gene product of herpes simplex virus (HSV) has been shown to play an important role in viral ...the UL56 protein are little ...the UL56 protein of HSV ... See full document
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Identification and characterization of the herpes simplex virus type 2 gene encoding the essential capsid protein ICP32/VP19c.
... Louis, Missouri 64303'; Division of Microbiology and Biotechnology, Wyeth-Ayerst Laboratories, Inc., Philadelphia, Pennsylvania 191013; and Department of Virus and Cell Biology, Merck Sh[r] ... See full document
11
Herpes Simplex Virus Type 1 Gene UL14: Phenotype of a Null Mutant and Identification of the Encoded Protein
... Herpes simplex virus type 1 (HSV-1) gene UL14 is located between divergently transcribed genes UL13 and UL15 and overlaps the promoters for both of these ...protein product have ... See full document
9
The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.
... Surprisingly, both AN and the UL12.5 protein were detected throughout the gradient. Moreover, a novel 54-kDa protein was also visible in these gradient fractions. This was deter- mined to be a proteolytic degradation ... See full document
9
The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... with 2 M GuHCl has been shown to result in selective removal of some capsid proteins without altering the icosahedral capsid shell ...with 2 M GuHCl remove VP21, VP22a, VP24, and VP26 ...with 2 M ... See full document
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Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene
... dishes of Vero cells by using the calcium phosphate technique (9, 32) or Lipofectamine 2000 (Life Technologies/Invitrogen, Carlsbad, CA), using the manufacturer’s recommended protocol. When the calcium phosphate tech- ... See full document
12
The UL25 Gene Product of Herpes Simplex Virus Type 1 Is Involved in Uncoating of the Viral Genome
... defective virus in1383 at the NPT ex- pressed high levels of  -galactosidase activity due to the re- lease of functional ts1249 VP16 into the ...for 2 h and transferred to 32°C for 18 ... See full document
13
Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.
... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document
11
The Us2 Gene Product of Herpes Simplex Virus 2 Is a Membrane-Associated Ubiquitin-Interacting Protein
... tail-anchored type II membrane protein that lacks lysine residues, to which ubiquitin molecules are most frequently attached (64, 66, ...key virus-cell interactions and reveal new targets for antiviral ... See full document
14
Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C.
... Anatomy of the herpes simplex virus type 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants.. [r] ... See full document
9
Identification and Characterization of a Herpes Simplex Virus Gene Product Required for Encapsidation of Virus DNA
... In this study, we report the characterization of an HSV-1 mutant that has a temperature-sensitive processing defect in the nucleocapsid structural polypeptide p40 and fails to encapsidat[r] ... See full document
9
Herpes simplex virus type 1 DNA synthesis requires the product of the UL8 gene: isolation and characterization of an ICP6::lacZ insertion mutation.
... Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characterization of an ICP6 lacZ insertion mutant..[r] ... See full document
9
Herpes Simplex Virus Type 2 UL56 Interacts with the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination
... a UL56-deficient derivative of wild-type HSV-2 (186), containing the lacZ gene in place of the UL56 ORF ...complete UL56 ORF was PCR amplified from wild-type HSV-2 ... See full document
14
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... for 2 weeks in selective medium, the cell population was assayed for ability to support plaque formation in the presence of bromode- oxyuridine (BUdR) by a virus stock derived from cotransfection of R7309 ... See full document
13
Mapping of the structural gene for the herpes simplex virus type 2 counterpart of herpes simplex virus type 1 glycoprotein C and identification of a type 2 mutant which does not express this glycoprotein.
... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document
7
Characterization of the gene encoding herpes simplex virus type 2 glycoprotein C and comparison with the type 1 counterpart.
... To obtain the complete nucleotide sequence of the region of the HSV-2 genome encoding gC, the protein encoded by the smaller 3'coterminal message, and the upstream and downstream regulat[r] ... See full document
9
Identification, transfer, and characterization of cloned herpes simplex virus invasiveness regions.
... Furthermore, we have shown that ANG DNA contains at least two distinct virulence regions tentatively designated INV-I and INV-II containing functions which can be transferred by DNA cotr[r] ... See full document
9
Herpes simplex virus UL56 interacts with and regulates the Nedd4 family ubiquitin ligase Itch
... Itch degradation is controlled because of its high stabi- lity, and moreover, the limited results obtained so far are controversial. One report showed that autoubiquiti- nated Itch is degraded by the proteasome [39], ... See full document
11
Identification of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... 818 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hybrid arrest of in vitro translation was used to localize the region of the herpes simplex virus type 1 genome enco[r] ... See full document
8
Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
... Protein extracts from the purified HSV-1 virions were separated on SDS-polyacrylamide gels, and the protein bands were probed in immunoblots with antisera directed against the UL37, ICP8[r] ... See full document
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