[PDF] Top 20 Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
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Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... of UL24 was to assemble clues to the function of UL24 during ...with UL24 mutations suggests that UL24 is involved, directly or indirectly, in one or several different membrane fusion ... See full document
8
Intracellular localization of the herpes simplex virus type 1 major transcriptional regulatory protein, ICP4, is affected by ICP27.
... Infected-cell protein 4 (ICP4) is the major transcriptional activator of herpes simplex virus (HSV) gene expression during productive ...gene expression induced by ICP4 and ... See full document
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Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.
... Regulation of the herpes simplex virus type 1 late gamma2 glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation [r] ... See full document
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Identification of a Highly Conserved, Functional Nuclear Localization Signal within the N-Terminal Region of Herpes Simplex Virus Type 1 VP1-2 Tegument Protein
... of virus infec- ...UL36-ve virus HSV-1 K ⌬ UL36 (5). This virus lacks the majority of the UL36 open reading frame and fails to grow on noncomplementing cell lines ...NLS expression ... See full document
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Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
... (nt) 1 and the SstI site designated nt 2421 as previously described (23), were digested at the BamHI site and the EcoRI site which occurs in the PEFR linker present in both ...residues 1 to 103, was joined ... See full document
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Herpes Simplex Virus Type 1 Gene UL14: Phenotype of a Null Mutant and Identification of the Encoded Protein
... Nuclear localization indicates that the UL14 protein could be incorpo- rated into particles in this ...tegument protein (the UL49 protein) to the cytoplasmic compartment implies that addition ... See full document
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Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27.
... the expression of cotransfected reporter genes in un- infected cells (4, 13, 41, 55, ...drive expression of the reporter genes (8, ...the protein-coding regions appear to mediate the effects of ... See full document
13
Temporal Regulation of Herpes Simplex Virus Type 2 VP22 Expression and Phosphorylation
... VP22 protein of herpes simplex virus type 2 (HSV-2) is a major component of the virion ...VP22 localization in infected ...a virus-dependent manner, (iii) that the HSV-2 ... See full document
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Membrane Association of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... chimeric protein was expressed and migrated at the expected molecular mass of approximately 64,000 ...intracellular localization of VP22 in transfected A7 cells was examined by live-cell fluorescence using ... See full document
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Intercellular Trafficking of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... Expression of VP22 in transfected or infected cells results in distribution of the protein through the neighboring cells where it accumulates in the ...nuclear localization of VP22 in non-expressing ... See full document
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PrPc Expression Influences the Establishment of Herpes Simplex Virus Type 1 Latency
... In our study we have utilized the TUNEL assay, which de- tects fragmented DNA, to measure apoptosis. It has been suggested that TUNEL identifies cells that have undergone not only apoptosis but also necrosis and ... See full document
12
Conserved Residues in the UL24 Protein of Herpes Simplex Virus 1 Are Important for Dispersal of the Nucleolar Protein Nucleolin
... the UL24 mutations on the distribution pattern seen for nucleolin in these ...the localization of nucleolin also varied depending on the UL24 substitution ...wild-type UL24, only ⬇ 16% ... See full document
11
A Mutation in the UL24 Gene Abolishes Expression of the Newly Identified UL24.5 Protein of Herpes Simplex Virus 1 and Leads to an Increase in Pathogenicity in Mice
... UL24.5neg virus in these ...wild-type virus vBAC_KOS, two independent isolates of the ...wild-type virus and rescue strains ...wild-type virus and for the respective ... See full document
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Early, Active, and Specific Localization of Herpes Simplex Virus Type 1 gM to Nuclear Membranes
... than virus-expressed gM (Fig. 2 and 3). The nuclear localization of gM was partic- ularly evident upon three-dimensional reconstruction of the data generated by confocal microscopy ...the expression ... See full document
14
Packaging Determinants in the UL11 Tegument Protein of Herpes Simplex Virus Type 1
... GFP expression vectors (2 to 20 g of DNA per 100-mm plate for each con- struct) using Lipofectamine 2000 ...equal expression levels, each vector was titrated prior to the start of the ...the ... See full document
8
Kinetics of expression of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... To characterize more thoroughly the kinetics with which the 65KDBP gene is expressed, we have examined not only the accumulation of 6SKDBP mRNA and protein, as compared to other well-cha[r] ... See full document
11
Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ... See full document
11
Molecular localization of abortive infection of resident peritoneal macrophages by herpes simplex virus type 1.
... In addition to localizing the molecular basis for the block in HSV-1 replication in B6C3F1 resident peritoneal macrophages to the expression of the early genes, we demonstrated that amon[r] ... See full document
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ICP0 Gene Expression Is a Herpes Simplex Virus Type 1 Apoptotic Trigger
... (Fig. 1, lane 7). In contrast, infection with d109, a recombinant virus devoid of all IE genes but expressing GFP from a heterologous promoter (53), yields results similar to those of mock infection ... See full document
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Incorporation of the Green Fluorescent Protein into the Herpes Simplex Virus Type 1 Capsid
... The herpes simplex virus type 1 (HSV-1) UL35 open reading frame (ORF) encodes a 12-kDa capsid protein designated ...fluorescent protein (GFP) was fused in frame ... See full document
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