[PDF] Top 20 Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination
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Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination
... The multiplex assay was tested on a col- lection of 316 bacterial isolates that included 309 Vibrio iso- ...309 isolates were 190 isolates representing the four target species, ... See full document
7
Use of Whole Genome Phylogeny and Comparisons for Development of a Multiplex PCR Assay To Identify Sequence Type 36 Vibrio parahaemolyticus
... parahaemolyticus sequence type 36 (ST36) strains that are native to the Pacific Ocean have recently caused multistate out- breaks of gastroenteritis linked to shellfish harvested from the Atlantic ...nonclade ... See full document
9
A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
... The PCR products for each target sequence from each set of primer pairs were also ...primers rpoB-F and rpoB-R, but other bacteri- al strains yielded no products, indicating that all mycobac- ... See full document
7
Simple Multiplex PCR Assay for Identification of Beijing Family Mycobacterium tuberculosis Isolates with a Lineage Specific Mutation in Rv0679c
... lineage identification by multiplex PCR. Multiplex PCR was developed targeting the Beijing-specific SNP on Rv0679c, employing a primer with the mutated nucleic acid at the 3= end of the ... See full document
8
Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... of PCR products (31, 50) is potentially more informative, this approach is beyond the scope of most diagnostic ...promising assay recently described by Pring-Åkerblom et ...on multiplex PCR ... See full document
8
Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real Time PCR
... our multiplex, real-time assay with clinical isolates, we blindly tested 314 isolates recovered from 255 distinct clinical specimens from 233 ...The isolates consisted of 254 cultures ... See full document
6
Genotypic Analyses of Vibrio parahaemolyticus and Development of a Pandemic Group Specific Multiplex PCR Assay
... 54 Vibrio parahaemolyticus strains including pandemic O3:K6 strains and newly emerged O4:K68, O1:K25, O1:K26, and O1:K untypeable strains (collectively referred to as the “pandemic group”) were examined for their ... See full document
7
Differential Identification of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNA Polymerase Gene (rpoB)
... (136-bp) rpoB DNA from NTM, restriction analysis and sequencing could be used for further species ...NTM isolates analyzed in this study were tentatively identified in accordance with this ...NTM ... See full document
5
Evaluation of Semiautomated Multiplex PCR Assay for Determination of Streptococcus pneumoniae Serotypes and Serogroups
... cps19fI sequence, with the forward and reverse primers positioned so that the last ...the multiplex PCR assay results for four clinical isolates (isolates PN1010, PN1008, PN1001, ... See full document
7
Rapid Multiplex PCR Assay for Identification of USA300 Community Associated Methicillin Resistant Staphylococcus aureus Isolates
... LNA PCR primer was designed with 6 AT repeats at the 3 ⬘ end and LNA-modified bases near the 5 ⬘ end to strongly drive correct hybridization and PCR amplification when used with an appropriate reverse ... See full document
6
Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR
... Korean isolates (GJ-1, GJ-2, BC, CN, and HS), which were isolated from the blood of infected humans and cows, were provided by ...The rpoB sequences of ...of rpoB DNA (359-bp) containing the rif ... See full document
7
Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland
... multilocus sequence typing (MLST) scheme with universal primer sets for ampli- fying and sequencing the seven target genes of Campylobacter jejuni and Campylobacter ...by sequence determination of ... See full document
12
Novel Multiplex PCR Assay for Detection of Chlorhexidine Quaternary Ammonium, Mupirocin, and Methicillin Resistance Genes, with Simultaneous Discrimination of Staphylococcus aureus from Coagulase Negative Staphylococci
... This multiplex assay was capable of differentiating staphylo- cocci from other bacteria while further distinguishing ...heptaplex assay was first optimized through assaying different primer ... See full document
8
Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe
... no sequence difference between the 16S rRNA gene in ...few sequence differences among the members of this group ...DNA sequence variations, it may not be pos- sible to use this area of the bacterial ... See full document
7
Development and Validation of a Multiplex PCR for Detection of Scedosporium spp in Respiratory Tract Specimens from Patients with Cystic Fibrosis
... this assay, we tested its ability to detect and identify Scedosporium ...the multiplex PCR assay correctly identified the targeted Scedosporium species with an overall sensitivity of ...for ... See full document
5
Picky eaters are rare: DNA based blood meal analysis of Culicoides (Diptera: Ceratopogonidae) species from the United States
... Vector-borne pathogens are significant threats to the health and welfare of both humans and animals worldwide [67]. More investigations into the ecology of all Culicoides species, not just the currently known vectors, ... See full document
9
The microbial ecology of Campylobacter jejuni in New Zealand within a spatial temporal framework : a thesis presented in partial fulfilment of requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatu, New Zealand
... jejuni isolates is a common finding [132][p59] and genetic analysis has shown there are significant differences between the isolates from agricultural animals (sheep, cattle and poultry) and those found in ... See full document
258
Rapid Detection of Mycobacterium tuberculosis Beijing Genotype Strains by Real Time PCR
... real-time PCR machine is able simultaneously to measure multiple dyes with distinct emission wavelengths, a multiplex PCR assay applying both PCRs in one reaction was ...the multiplex ... See full document
6
Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay
... with PCR-RFLP markers (17). Atypical isolates were characterized by a different mix of alleles of haplogroups 1 to 3 and by additional atypical alleles at some ...lated isolates within one clonal ... See full document
5
A Multiplex Real Time PCR Assay for Identification of Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii in Samples from AIDS Patients with Opportunistic Pneumonia
... Design of primers and probes. Primers and molecular beacon probes were designed to specifically amplify a region of the ITS of the rDNA from Cryptococcus neoformans/C. gattii, Histoplasma capsulatum var. capsula- tum and ... See full document
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