[PDF] Top 20 Influence of substrate structure on disintegration activity of Moloney murine leukemia virus integrase.

... The disintegration endonuclease product results from cleavage of the LTR from the LTR-target sequence junction and can be detected with substrates having a 5'-end-labeled B strand right [r] ... See full document

... the virus life cycle, suggesting that the IN protein may have other functions in addition to its integration ...immunodeficiency virus type 1 IN protein is required for efficient viral DNA synthesis and ... See full document

... transfer activity of ND105 protein was severely com- promised by alkylation ...transfer activity indicates that residue C209 in the central catalytic core is NEM ...maintained activity, albeit ... See full document

... dumbbell substrate similar to that described by Vincent et ...assay disintegration activity (oligonucleotide db-y-m, sequence 5 9 -TGAAAGTTCTTTCAGGCCCT TGGGCCGGCGCTTGCGCC-3 9 ; ...The ... See full document

... nonreplicative virus with the mutant RT having only 5% of the wild-type RNase H activity in an in situ RNase H ...to substrate but also conforms and bends RNA/DNA substrate to optimize DNA ... See full document

... end disintegration substrate lacking the 59-ss tail in the viral LTR (untailed ...this substrate, the reaction is dependent on both the catalytic core and the HHCC domain (16) ...untailed ... See full document

... of substrate recog- nition and multimeric M-MuLV IN functions by using a bimo- lecular, coordinated-disintegration ...of disintegration at either or both LTR- target DNA junctions (single and double ... See full document

... LTR substrate with respect to IN 1–105 or IN 106–404, surprisingly, altered the preference for the two-end concerted ...binding activity, preincubation of the viral termini with the HHCC favors the ... See full document

... helicase activity [55], is thought to be the first protein to bind to a DNA double strand break [56], func- tions as a sliding clamp on DNA and recruits DNA-PK cs , DNA polymerases, and ligases to the site of ... See full document

... standard substrate in their respective reaction ...and disintegration assays were performed concurrently with the same batch-treated M-MuLV IN ...and disintegration assays were repeated three times ... See full document

... Integration of retroviruses and retrotransposons into the host DNA displays various degrees of target site specificity. A number of factors, including the local DNA structure and the proteins bound to the DNA, ... See full document

... To determine whether the region was involved in the observed differential recognition, the Cys residues of M-MuLV IN were modified with NEM and assayed for the endonuclease activity of d[r] ... See full document

... the structure be- tween PAL1 and PAL2 is different in the two RNAs and that this region forms the SL0 stem-loop only in the genomic RNA (structural differences between the ex virio and in vitro dimers are ... See full document

... In these experiments, the first cells infected in the bone marrow of neonatal mice inoculated i.p. with M-MuLV were characterized. The use of a replication-defective M-MuLV- based vector allowed the identification of ... See full document

... Equilibrium sedimentation data yield molecular weights which are independent of conformation 32, and the method has been shown by Tashiro and Yphantis 33 to be practical for the determin[r] ... See full document

... MoMLV long terminal repeat (LTR) promoter and enhancer and the b-galac- tosidase reporter gene attached to a nuclear location signal (nlslacZ) under the control of a truncated Rous sarcoma virus (RSV) LTR. VSV-G ... See full document

... 2843 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest On the basis of earlier studies with both detergent-disrupted virions the endogenous reaction and an in vitro recon[r] ... See full document

... In vitro studies using oligonucleotides containing sequences of viral DNA ends showed that purified integrase of human immunodeficiency virus type 1 HIV-1, murine leukemia virus MLV, or [r] ... See full document

... 464-473 0022-538X/80/05-0464/10$02.00/0 Suppression of Murine Retrovirus Polypeptide Termination: Effect of Amber Suppressor tRNA on the Cell-Free Translation of Rauscher Murine Leukemia[r] ... See full document

... Rat NRK-5 cell DNA was prepared as described by 164 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest By screening a library of unintegrated, circular Moloney murine leuk[r] ... See full document