[PDF] Top 20 Interaction of the Human Immunodeficiency Virus Type 1 Nucleocapsid with Actin
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Interaction of the Human Immunodeficiency Virus Type 1 Nucleocapsid with Actin
... 41). Virus production analysis by immunoblotting. Virus produced from H9 cells over a period of 24 h was ...NaCl, 1% Triton X-100, 1% sodium deoxycholate, ...approximately 1/1,000 of ... See full document
8
Basic Residues in Human Immunodeficiency Virus Type 1 Nucleocapsid Promote Virion Assembly via Interaction with RNA
... via interaction with the provirally encoded Gag. (C) HA-Gags, wild type or bearing the NC mutations indicated, were coexpressed with protease-defective HIV-1 ... See full document
12
Development of a Cell-Based Assay Probing the Specific Interaction between the Human Immunodeficiency Virus Type 1 Nucleocapsid and Psi RNA In Vivo
... the interaction with ⌿ RNA and thus validate the specificity of our assay further, we employed three kinds of NC zinc finger mu- tants (a gift of ...FIG. 1. Translation inhibition caused by the HIV ... See full document
5
The Interaction of APOBEC3G with Human Immunodeficiency Virus Type 1 Nucleocapsid Inhibits tRNA3Lys Annealing to Viral RNA
... The minus-strand DNA that is made in the presence of hA3G contains 1 to 2% cytosines mutated to uracil (23, 35, 38, 51). Because hA3G is a cytidine deaminase (38, 47, 51), it has been suggested that the decreased ... See full document
10
Divergent Bro1 Domains Share the Capacity To Bind Human Immunodeficiency Virus Type 1 Nucleocapsid and To Enhance Virus-Like Particle Production
... Gag-Gag interaction sites in the N-terminal half of the Gag precursor and thus was presumably attenuated in its ability to bend the plasma membrane away from the ... See full document
9
Elimination of Protease Activity Restores Efficient Virion Production to a Human Immunodeficiency Virus Type 1 Nucleocapsid Deletion Mutant
... relative to the wild type) and severely genomic RNA deficient. Immunoblot and high-pressure liquid chroma- tography analyses showed that all of the mature Gag proteins except NC were present in the mutant virion ... See full document
10
Rescue of Multiple Viral Functions by a Second-Site Suppressor of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation
... NC plays roles in nearly all steps of the viral life cycle. As a domain within the Gag polyprotein, NC specifically binds and incorporates viral genomic RNA into virions (reviewed in ref- erences 5 and 63) and drives ... See full document
11
Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides
... stable interaction with NC ...stable interaction is somewhat lower than 7 or 8, the value found in a number of studies for the “occluded site size,” or average number of bases per NC molecule when a nucleic ... See full document
8
Time Resolved Fluorescence Investigation of the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein: Influence of the Binding of Nucleic Acids
... The parameters calculated by Eqs. 5 and 6 are given in Table 1 and are in very good agreement with the experi- mental values. This is strongly in favor of the linear com- bination hypothesis and suggests that ... See full document
10
Amino-Terminal Region of the Human Immunodeficiency Virus Type 1 Nucleocapsid Is Required for Human APOBEC3G Packaging
... for human APOBEC3G packaging into human immunodeficiency virus type 1 (HIV-1) ...Gag virus-like particles (VLP) that lacked all the viral genomic RNA packaging ... See full document
12
Identification and characterization of a new type of inhibitor against the human immunodeficiency virus type 1 nucleocapsid protein
... for 1 h 30 ...noninfectious virus production generated by A1752 would most likely be attributable all to the spe- cific interaction of A1752 with NC, which inhibited the NC chaperone function and led ... See full document
15
Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression
... change in HIV-1 early spliced mRNA expression occurs after the HIV-1 provirus integration into the cellular genome. Den- sitometer readings of the HIV-1 DNA bands reveal that sim- ilar amounts of ... See full document
11
Requirement for an Intact T-Cell Actin and Tubulin Cytoskeleton for Efficient Assembly and Spread of Human Immunodeficiency Virus Type 1
... At 1 h, an increase of ...gp120-CXCR4 interaction (AMD3100) resulted in values that were signifi- cantly lower (P ⬍ ...with actin-depolymerizing agents cytochalasin D and latrunculin and the ... See full document
14
Actin Associates with the Nucleocapsid Domain of the Human Immunodeficiency Virus Gag Polyprotein
... of actin-binding proteins in this interaction cannot be ruled out, since ezrin and moesin, which are actin mem- brane associated, and cofilin, a small actin-severing protein, are present in ... See full document
10
Evidence for a Stable Interaction of gp41 with Pr55Gag in Immature Human Immunodeficiency Virus Type 1 Particles
... NaCl, 1 mM ...containing 1% Triton ...with 1% Triton X-100 for 1 h at either 4 or 37°C and then layering them directly onto a 30 to 70% linear sucrose ...Eleven 1-ml fractions were ... See full document
7
DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.
... 9 DNA Strand Exchange and Selective DNA Annealing Promoted by the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein ZENTA TSUCHIHASHIt AND PATRICK 0.. BROWN* Howard Hughes Medical[r] ... See full document
8
Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.
... The individual proximal cysteine-histidine box mutations were exchanged for the wild-type sequences in the Pr55gag expression construct to determine whether binding to HIV-1 RNA in vitro[r] ... See full document
11
Human Immunodeficiency Virus Type 2 Vpx-Gag Interaction
... overnight at 4°C in the presence of 40 ml of glutathione beads and then washed three times with 13 lysis buffer. Proteins were eluted off the beads by the addition of 23 sample buffer and heated to 95°C for 5 min. They ... See full document
5
The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivity.
... Sucrose density gradient analysis of HIV-1 virions. Twelve milliliters of su- pernatant was harvested from transfected cell cultures, clarified by centrifugation at 3,000 rpm for 15 min, and concentrated by ... See full document
7
Interaction of Human Immunodeficiency Virus Type 2 Vpx and Invariant Chain
... selection for histidine, leucine, and tryptophan prototrophy. -Galactosidase activity was assessed on nitrocellulose filter replicas of yeast transformants (9), and three colonies that expressed high levels of ... See full document
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