[PDF] Top 20 Large-Scale Analysis of Adeno-Associated Virus Vector Integration Sites in Normal Human Cells
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Large-Scale Analysis of Adeno-Associated Virus Vector Integration Sites in Normal Human Cells
... of integration events from nor- mal human cells without selection in a scalable process, with automated plasmid purification and junction ...leukemia virus and human immunodeficiency ... See full document
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Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... simplex virus type 1 (HSV-1)-based amplicon vectors have a large transgene capacity and can efficiently infect many different cell ...on adeno-associated virus (AAV) can either persist ... See full document
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Efficient Site-Specific Integration of Large Transgenes by an Enhanced Herpes Simplex Virus/Adeno-Associated Virus Hybrid Amplicon Vector
... the integration mechanism, nested PCR was performed to amplify the integration junc- tions, followed by sequencing ...⫹ cells that were infected by HLIGp5-R at an MOI of 1 was extracted 48 h after ... See full document
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Packaging of Human Chromosome 19-Specific Adeno-Associated Virus (AAV) Integration Sites in AAV Virions during AAV Wild-Type and Recombinant AAV Vector Production
... Adeno-associated virus (AAV) has evolved a biphasic life cycle to ensure persistence in its primate ...helper virus, adenovirus (Ad), or herpesvirus for productive infection ...preferential ... See full document
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Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... from integration. Our Southern blot analysis results showed that this happened in most but not all of the ...the integration of the rep gene is Rep-induced tox- icity to the transduced cells ... See full document
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A High-Capacity, Capsid-Modified Hybrid Adenovirus/Adeno-Associated Virus Vector for Stable Transduction of Human Hematopoietic Cells
... into human HSCs is still problematic. We have developed a hybrid Ad-AAV vector with a chimeric adenoviral capsid for efficient gene transfer into human hematopoietic ...This vector is devoid ... See full document
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Substitution of adeno associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences
... plasmid-transfected cells was analyzed by using the Southern blotting ...pSub201(-) vector used to make AAV2 containing two wild type ITRs is shown in Figure ...the vector. The vector maps for ... See full document
11
Comparative analysis of the transduction efficiency of five adeno associated virus serotypes and VSV G pseudotype lentiviral vector in lung cancer cells
... apies. Adeno-associated virus and lentivirus have been widely studied and used for gene ...delivery vector plays an important role in the ultimate success rate of the gene therapy ...the ... See full document
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Adeno-Associated Virus Type 2 Induces Apoptosis in Human Papillomavirus-Infected Cell Lines but Not in Normal Keratinocytes
... Under normal conditions, nicking of cellular chromatin results from the inherent site- specific endonuclease activity of Rep78/Rep68, a function re- quired for AAV replication and integration into the ... See full document
7
Identification and Characterization of an Adeno-Associated Virus Integration Site in CV-1 Cells from the African Green Monkey
... or human sequences were almost identical in size, sug- gesting that the numbers of Rep molecules forming the protein complex are ...the human RBE, the simian RBE, or nonspecific DNA (2, 57, ...be ... See full document
12
Structure of Adeno-Associated Virus Vector DNA following Transduction of the Skeletal Muscle
... Human adeno-associated virus (AAV) type 2 is a nonpatho- genic parvovirus, whose ...the virus life cycle, and cap, encoding structural virion proteins ...infected cells, the ... See full document
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Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... site-specific integration was determined to range from 10 to 40%, which is somewhat lower than efficien- cies reported in earlier ...Southern analysis or FISH, whereas we used a PCR-based assay that may ... See full document
8
Potent inhibition of human immunodeficiency virus type 1 in primary T cells and alveolar macrophages by a combination anti-Rev strategy delivered in an adeno-associated virus vector.
... in human immunodeficiency virus type 1 (HIV-1) pathogenesis and disease progression as it outstrips the capacity of the immune system to ...cellular sites for HIV-1 production include T lymphocytes ... See full document
10
Novel Adeno-Associated Virus Vector Vaccine Restricts Replication of Simian Immunodeficiency Virus in Macaques
... T cells) after a single dose of ...in large- scale vaccine efficacy ...postchallenge virus loads (compared to study B) suggested that the DNA priming doses had an ... See full document
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Adeno-associated virus vector integration junctions.
... between vector integration in our system and integration by wild-type ...random vector integration that we observed, two- thirds of wild-type proviruses studied in latently infected hu- ... See full document
8
Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver
... Recombinant adeno-associated virus serotype 2 (rAAV2) vectors have been considered safe and are widely used for delivering therapeutic genes into experimental animals and human subjects ... See full document
9
Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus–Adeno-Associated Virus Vector
... polyhedrosis virus as a vector for the delivery of genes into mammalian cells has been reported (8, ...insect cells (35) or as biopesticides (12), it was shown that ...drosis virus can ... See full document
10
Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185
... knockdown cells were infected with AAV at a multiplicity of infection of ...site-specific integration becomes detectable between 8 and 16 h postinfection (11), total DNA was prepared 16 h postin- fection ... See full document
12
Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
... transgenic cells somehow contrasts with the finding that the ITR-AAVS1 junctions are clustered more closely to the RBS around position 400 when the ...targeted integration could involve the formation of a ... See full document
10
Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... target cells (30, ...minute virus of mice, have been conducted. Minute virus of mice was reported to bind to both specialized (coated pits) and unspecified regions of the cell membrane, and ... See full document
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