[PDF] Top 20 Lithium influences differentiation and tissue specific gene expression of mouse embryonic stem (ES) cells in vitro

... (Konecki et al., 1982) as well as β -tubulin (see Rohwedel et al., 1998) were used as internal standards. Reverse transcription was performed with MuLV reverse transcriptase (Perkin-Elmer) for 1 hour at 42 ° C, followed ... See full document

... in ES-derived pancreatic cells. The neural-specific protein Nnat annotated for neuronal differentiation and function [see (Chu and Tsai, 2005)] was selected to discriminate between neural and ... See full document

... E14.1 ES cells was according to standard procedures (Matise et ...targeted ES cell clones were identified and confirmed by Southern blot analysis using specific hybridisation probes (Kist et ... See full document

... specialized cells of an organism arise from a single- cell zygote, variation in gene expression is attributed to differences at the epigenetic ...heritable gene function that does not entail a ... See full document

... hepatic differentiation of hESC in vitro with and without additional growth ...hepatocytes-like cells. Recently, it has been reported that mouse ES cells can generate ... See full document

... Stem cells provide, potentially, an unparalleled opportu- nity for treatment of any number of degenerative condi- ...of stem cell differentiation and of signalling pathways activated therein, ... See full document

... in expression of pluripotency-associated genes prompted us to look at markers of ...female cells because the XX cells are slightly delayed, as reported in a previous study ...male cells to be ... See full document

... control embryonic stem cell identity, they found that reprogramming of mouse and human embryonic fibroblasts (MEF) into induced pluripotent stem cells (iPSCs) can be achieved by ... See full document

... ABSTRACT Embryonic stem cell studies have generated great interest, due to their ability to form a wide variety of matured ...of embryonic stem cells (ESCs) and of the genes they ... See full document

... selective expression of one or more specific genes in the blastomeres of preimplantation embryos triggers their differentiation into either trophectoderm or ...to embryonic stem ... See full document

... mES cells [29, ...and embryonic development [3, 8]. Specifically, the differentiation of myoblasts was also shown to require Rictor/mTORC2 ...and tissue damages after myocardial infarction ... See full document

... Npas4 expression in mESCs undergoing ...Npas4 expression during embryonic de- velopment and neural differentiation of ESCs, the 46C mESC line was selected as the parental cell line for Npas4 ... See full document

... of tissue architecture before a reasonable understanding of organ or tissue formation can be ...the specific study of cell differentiation historically has been advanced by molecular analyses ... See full document

... atein vitro and generate oocytes both in vivo and in vitro ...germ cells that express cell-surface RNA heli- case, Vasa, a marker of adult human oogonial stem ...nial stem cells ... See full document

... using specific antibodies (MeDIP/hMeDIP) (Ficz et ...at specific genomic loci provides a quantitative read-out of cytosine modifications at that locus and allows comparative analysis between conditions or ... See full document

... embryonic cells. Higher concentrations also promote faster differentiation as shown by the pattern of expression of Oct3/4 which got down-regulated more rapidly in embryoid bodies that were ... See full document

... in vitro as a source of growth factors (GFs) and other elements essential for cell adhesion and proliferation ...in vitro expansion [14 – ...in- vitro cell expansion ...mesenchymal stem ... See full document

... Int J D BioI 41 235 243 (1997) 235 Reflections on the biology of embryonic stem (ES) cells RICHARD L GARDNER' and FRANCES A BROOK Department of Zoology, University of Oxford, Oxford, United Kingdom AB[.] ... See full document

... fixed cells were permeabilized with either ...The cells were washed with in PBS2A four times for 10 minutes and blocked with 5% normal goat serum in PBS2A for 20 minutes followed by an 16 hour, 4 o C ... See full document

... day mouse em- bryos were used as feeder ...When ES cells were cultured in absence of feeder layer recombinant leukemia inhibitory factor (LIF) was added into the culture media (Sigma, USA) to final ... See full document