[PDF] Top 20 Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
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Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), belongs to a class of integral membrane proteins ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the C-terminal 18 amino acids of NS5B have any effects on in vitro RdRp activity under the conditions used in this ...D(⫹) RNA as a template. It was found that the C-terminal 18-amino-acid truncation ... See full document
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Complete replication in vitro of tobacco mosaic virus RNA by a template-dependent, membrane-bound RNA polymerase.
... template-dependent RNA polymerase by antibodies to the TMV-encoded 126- and 183-kDa ...the virus RNA replication, a 126-kDa protein containing amino acid sequence motifs typical ... See full document
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Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
... genomic RNA with or without the conserved 98-nucleotide tail. The NS5B-RNA complexes were specifically competed away by the unlabeled homologous RNA but not by the viral 5 ⴕ noncoding region and very ... See full document
6
Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
... catalyze RNA polymerization on both HCV-specific RNA and nonviral RNA templates (2, 11, 13, 17, 21, ...HCV RNA in vivo ...HCV RNA replication in the ...HCV RNA ... See full document
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Interaction with a Ubiquitin-Like Protein Enhances the Ubiquitination and Degradation of Hepatitis C Virus RNA-Dependent RNA Polymerase
... Hepatitis C virus (HCV), a member of the Flaviviridae fam- ily, is the major causative agent of non-A, non-B hepatitis ...single-stranded RNA genome en- codes a single polyprotein of ... See full document
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Two Membrane-Associated Regions within the Nodamura Virus RNA-Dependent RNA Polymerase Are Critical for both Mitochondrial Localization and RNA Replication
... It is difficult to simply extrapolate the localization of the NoV RdRp from that of FHV, since the two proteins are markedly dif- ferent. Although both contain canonical RdRp domains, they share only 44% sequence ... See full document
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Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
... The truncated proteins were further subjected to a more stringent solubility test by ultracentrifugation at 100,000 3 g for 30 min. The results (Fig. 2, lanes 3 and 4 and 6 and 7) demonstrated that the truncated proteins ... See full document
6
Zinc Salts Block Hepatitis E Virus Replication by Inhibiting the Activity of Viral RNA-Dependent RNA Polymerase
... ABSTRACT Hepatitis E virus (HEV) causes an acute, self-limiting hepatitis in healthy individuals and leads to chronic disease in immunocompromised ...the virus usually causes sporadic ... See full document
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Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
... characteristic membrane localization pattern of NS5A was observed ...cytoplasmic membrane structures was ...toplasmic membrane localization of ... See full document
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Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... Plasmid constructions. Standard recombinant DNA techniques were used to generate all the constructs (48). All HCV fragments were cloned from a chron- ically infected patient and belong to genotype 1b (5). The basic ... See full document
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Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication
... ER membrane, as judged by association with ER-retained calnexin and NS5A ...HCV RNA synthesis, in which genome replication occurs within ER-derived vesicles of the membranous web whose ... See full document
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Host Factors Associated with the Sindbis Virus RNA-Dependent RNA Polymerase: Role for G3BP1 and G3BP2 in Virus Replication
... early replication complexes, the failure to identify UNC84, PRMT5, SFRS10, and ANXA1 in previous studies is rather ...viral RNA replication. The finding of five factors in association with ... See full document
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Hydrophobic and Charged Residues in the C-Terminal Arm of Hepatitis C Virus RNA-Dependent RNA Polymerase Regulate Initiation and Elongation
... The RNA-dependent RNA polymerase (RdRp) of hepatitis C virus (HCV) is essential for viral genome ...two C-terminal features, a -loop and a C-terminal ... See full document
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Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... Hepatitis C virus (HCV) is a single-stranded, positive-sense RNA ...an RNA-depen- dent RNA polymerase (RdRp) that is responsible for replica- tion of viral RNA ... See full document
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De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
... Hepatitis C Virus (HCV), a member of the flaviviridae family of positive-strand RNA viruses, infects up to 3% of the world’s population ...the RNA-dependent RNA ... See full document
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Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals
... initiate RNA synthesis by a number of ...influenza virus, can use a combination of the two strategies, depending on the RNA to be synthesized ...Flaviviridae RNA-dependent RNA ... See full document
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A Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase Capable of Copying the Full-Length Viral RNA
... HCV RNA in a primer-independent manner. First, the kinetics of RNA synthesis by this RdRp using the full-length HCV RNA as a template indicated that the NS5B-mediated RNA synthesis is not ... See full document
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Regulation of De Novo-Initiated RNA Synthesis in Hepatitis C Virus RNA-Dependent RNA Polymerase by Intermolecular Interactions
... faster replication kinetics in cell culture than the replicon from genotype 1b ...The C-terminal His tag in ⌬ 21 does not affect de novo ...in RNA synthesis ...nitrocellulose membrane probed ... See full document
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Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase
... the C-terminal 31 amino acids of NS5B derived from an infectious GBV-B cDNA clone (4) (kindly provided by Jens Bukh, University of Copenhagen, Den- mark) via a serine-glycine linker sequence to the C ... See full document
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