[PDF] Top 20 Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures
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Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures
... of tuberculosis can be greatly facil- itated by the application of strain-specific ...type Mycobacterium tuberculosis complex strains, most molec- ular methods are based on the ... See full document
5
Discriminatory Power and Reproducibility of Novel DNA Typing Methods for Mycobacterium tuberculosis Complex Strains
... techniques, IS6110 RFLP, mixed-linker PCR, FLiP, and VNTR typing, are highly reliable, discrimina- tive, and appropriate for epidemiological typing of strains of the ...M. ... See full document
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Stability of Variable Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis
... VNTR typing systems for M. tuberculosis complex strains made use of very limited sets of loci (6, 8, 14, 15, 17), which turned out not to be sufficiently discriminatory ...system based ... See full document
6
Utility of New 24 Locus Variable Number Tandem Repeat Typing for Discriminating Mycobacterium tuberculosis Clinical Isolates Collected in Bulgaria
... molecular typing of Mycobacterium tuberculosis with a collection of strains circulating in ...133 strains from epidemiologically unlinked patients from different regions ... See full document
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Variable Number Tandem Repeat Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110 by Using Mycobacterial Interspersed Repetitive Units
... of PCR-based typing methods—rapid turnaround, no requirement for large quantities of DNA, and the ability to use nonviable samples—and in addition provides a simple dig- ital ...during ... See full document
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Genetic Diversity of Mycobacterium tuberculosis Isolates from a Tertiary Care Tuberculosis Hospital in South Korea
... several PCR-based methods have been developed to discriminate among strains, including spoligotyp- ing and mycobacterial interspersed repetitive unit-variable number tandem repeat typing ... See full document
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Evaluation of Semiautomated IS6110 Based Restriction Fragment Length Polymorphism Typing for Mycobacterium tuberculosis in a High Burden Setting
... RFLP method rather than the semiautomated system, as the electropho- resis conditions are optimized with larger gels and a longer dura- tion of electrophoresis ...the use of the semiautomated system to ... See full document
6
Use of Variable Number Tandem Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping
... family strains of RFLP cluster R2 exhibited iden- tical spoligo patterns, but they had VNTR patterns that dif- fered by two repeats at MIRU26 and three repeats at ...family strains by VNTR, six were ... See full document
7
Polymorphism of Variable Number Tandem Repeats at Multiple Loci in Mycobacterium tuberculosis
... fingerprinting method based on restric- tion fragment length polymorphism (RFLP) of IS6110 inser- tion sequences has been established as the standard for typing strains of ... See full document
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Identification of a New DNA Region Specific for Members of Mycobacterium tuberculosis Complex
... successful use of DNA amplification for the detection of tuberculous mycobacteria crucially depends on the choice of the target sequence, which ideally should be present in all tuberculous mycobacteria and absent ... See full document
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Comparison of a Semiautomated Commercial Repetitive Sequence Based PCR Method with Spoligotyping, 24 Locus Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat Typing, and Restriction Fragment Length Polymorphism Based Analysis of IS6110 for Mycobacterium tuberculosis Typing
... M. tuberculosis stricto sensu clinical isolates and the H37Rv reference strain (French National Reference Center for Mycobacteria) were ...and IS6110-RFLP, including three East African-Indian (EAI) ... See full document
5
Direct identification and typing of Mycobacterium tuberculosis by PCR
... M. tuberculosis employing a rationale the same as ours was published by Ross and Dwyer (22) while this work was under way; however, those investigators used different PCR primers and amplification ...in ... See full document
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Mycobacterial Interspersed Repetitive Unit Typing of Mycobacterium tuberculosis Compared to IS6110 Based Restriction Fragment Length Polymorphism Analysis for Investigation of Apparently Clustered Cases of Tuberculosis
... The use of polymorphism in the number of copies of tandem repeat sequences is an invaluable tool for the genotyping of higher ...M. tuberculosis and, notably, Mycobacterium bovis BCG ...repeat ... See full document
7
Molecular Characterization of Mycobacterium tuberculosis Isolates from Different Regions of Bulgaria
... oligonucleotide typing) technique (10). This method has been widely applied to study the molecular epidemiology and evolutionary genetics of ...is PCR-based, it requires less DNA than ... See full document
5
Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains
... RFLP typing method is, however, laborious and requires the preparation of several micrograms of chro- mosomal DNA, a procedure which may take several weeks because of the slow growth rate of ...alternative ... See full document
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Genome Sequence Based Fluorescent Amplified Fragment Length Polymorphism Analysis ofMycobacterium tuberculosis
... between strains from unlinked ...for IS6110 typing and spoli- ...whose IS6110 type was the same (iso- lates 114 and ...group method with averages, parsimony, and maximum ... See full document
6
Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
... mixed-linker method originally published in 1993 has been successfully applied in several outbreak investigations and population-based studies (6–9, 15, 19, ...between strains of M. ... See full document
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Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosisStrains in Actual Chains of Transmission
... M. tuberculosis using gene probes (ACCUProbe; GenProbe, San Diego, ...proportion method on Lo¨wenstein-Jensen medium and/or the modified proportion method in the BACTEC 460TB system (Becton ... See full document
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Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism
... for IS6110 has been used widely for retro- spective epidemiological studies (5, 7, 9, ...its use in the management of patients with acute clinical cases of infection or in rapidly responding to an ...a ... See full document
5
Optimization of Standard In House 24 Locus Variable Number Tandem Repeat Typing for Mycobacterium tuberculosis and Its Direct Application to Clinical Material
... VNTR typing by the optimized 24-locus VNTR method was tested with DNA from serial dilu- tions of the four ...M. tuberculosis strains in ...M. tuberculosis strains used to ... See full document
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