[PDF] Top 20 Multilaboratory Evaluation of Real Time PCR Tests for Hepatitis B Virus DNA Quantification
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Multilaboratory Evaluation of Real Time PCR Tests for Hepatitis B Virus DNA Quantification
... Another difference that was observed pertained to false- positive rates. Assays that relied on manual extraction (High- Pure TaqMan and artus HBV) had higher false-positive rates than did those that employed automated ... See full document
5
Development of a TT Virus DNA Quantification System Using Real Time Detection PCR
... TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains ...a quantification system was developed. TTV DNA was quantified by a ... See full document
5
COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... We demonstrated here that CAP-CTM can be applied to specimens other than plasma, allowing the detection of cell- associated HBV DNA. This is of particular value in the context of occult-HBV infection in ... See full document
7
Application of real time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia
... Hepatitis B virus (HBV) is the leading cause of viral hepa- titis in humans ...the virus, 60 mil- lion of them residing in Africa ...of virus in the ...of hepatitis B ... See full document
7
Evaluation of Real Time PCR Laboratory Developed Tests Using Analyte Specific Reagents for Cytomegalovirus Quantification
... CMV DNA from plasma after a course of ganciclovir therapy increases the risk of relapsing CMV infection ...CMV DNA in plasma is longer for patients who relapse than for those who do not ... See full document
5
Comparison of Real Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy
... the quantification of HBV DNA, have become available, more studies will be needed to compare the performance characteristics of the RealART as- say with those of these more sensitive assays for the ... See full document
6
Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... and quantification of hepatitis B virus (HBV) DNA are essential for the diagnosis and treatment of chronic HBV ...of real-time PCR assays for HBV DNA ... See full document
7
Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... HBV DNA levels (4, 5, 12, 13, 26, ...HBV DNA level in patients infected with a single genotype was higher than that of patients infected with a mixture of genotypes in the chronic hepatitis B ... See full document
8
Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments
... diagnostic PCR assays, the use of ICs is necessary to demonstrate the absence of PCR inhibition ...As PCR inhibitors are not always reliably removed from clinical speci- mens during DNA ... See full document
6
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... of PCR. As an alternative to synthetic fragments of RNA or DNA, housekeeping genes or different pathogens are used as an internal ...the PCR assay than in vitro-transcribed RNA ... See full document
8
Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients
... The quantification of hepatitis C virus (HCV) RNA is es- sential for the management of chronic hepatitis C therapy based on the combination of pegylated interferon (PEG-IFN) and ribavirin ... See full document
5
Performance Characteristics and Comparison of Abbott and artus Real Time Systems for Hepatitis B Virus DNA Quantification
... Abbott real-time HBV assay and European Conformity (CE)-marked IVD-licensed artus assay for quantification of HBV ...artus real-time PCR were processed by two purification ... See full document
7
Comparison of Conventional PCR with Real Time PCR and Branched DNA Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5
... and DNA polymerase activ- ities. The quantification standard represents a noninfectious RNA construct containing fragments of HCV sequences with primer binding regions identical to those of the HCV 5⬘ ... See full document
9
A Genotype Independent Real Time PCR Assay for Quantification of Hepatitis B Virus DNA
... Accurate quantification of hepatitis B virus (HBV) DNA levels is important for monitoring patients with chronic HBV infection and for assessing their responses to antiviral ...a ... See full document
6
Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... of hepatitis B virus (HBV) DNA in peripheral blood is a reliable marker of active HBV ...HBV DNA quantification is the best method for monitoring the level of HBV replication in ... See full document
8
Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
... assays. Tests with the COBAS AMPLIPREP Total Nucleic Acid Isolation kit (Roche) for isolation of nucleic acids, tests with the RealArt HBV LC PCR Reagents (Artus), and the COBAS AMPLICOR HBV MONITOR ... See full document
5
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... group B consisted of 59 plasma samples obtained from patients at Beijing ChaoYang Hospital between July 2016 and Au- gust ...HBV DNA positive plasma samples obtained from blood banks in China. HBV ... See full document
9
Quantitation of Hepatitis B Virus Genomic DNA by Real Time Detection PCR
... HBV DNA by RTD-PCR. The principle of RTD-PCR is as ...during PCR, the probe specifically anneals between the forward and reverse ...before PCR. ⌬Rn increases during PCR as the ... See full document
5
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... novel real-time PCR assay is shown in Table ...HBV DNA assays might be insufficient for detecting very low HBV DNA levels typical in patients undergoing antiviral therapy and those with ... See full document
9
Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... on hepatitis B virus infection (7, 22, 29), especially in the United States, Europe, and Africa (on genotypes A and D through H), are therefore needed, because most studies to date have been ... See full document
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