[PDF] Top 20 Multiple Blocks to Human Immunodeficiency Virus Type 1 Replication in Rodent Cells

... of human gene products that are required for early steps in the human immuno- deficiency virus type 1 (HIV-1) life cycle has raised the possibility that rodents might be ... See full document

... Reagents. (i) Antibodies. To characterize both TEC and thymocyte popula- tions, monoclonal antibodies (MAbs) against CD1a (B17.20.9), CD2 (39C1.5), CD3 (X35), CD14 (RMO52), CD16 (3G8), CD19 (J4 119), CD45 (ALB12), ... See full document

... impair virus entry into ...infectious virus particles to the target cell by efficient binding of the Fc region of IgG to Fc␥R and by binding of Fab regions to the HIV ...of virus replication, ... See full document

... Ebola virus, Marburg virus, and measles virus, but not ...Ebola virus, Marburg virus, measles virus, and reovirus ...Vero cells. Rab9 mRNA expression in mock- or ... See full document

... T cells and mac- rophages, although the enhancing effect of NO was observed with X4 but not with R5 ...HIV-1 replication in macrophages ...T-tropic virus, supplies different signals than CCR5 ... See full document

... for 1 h at 37°C. After 1 h, monensin (BD; 1 ␮l/ml) was added and the cells were cultured for another 4 to 5 h at ...the cells were collected, stained with anti-CD8-APC (BD), and ... See full document

... infectious virus. For PCR, DNA was extracted from cells on days 1, 3, 7, and 14 following HHV-6 infection by using a kit as recommended by the manufacturer (Stratagene, La Jolla, ...for 1 min ... See full document

... lymphoid cells residing in the mucosal microenvironment ...epithelial cells obtained from saliva ...epithelial cells bearing HIV-1 DNA sequences ...The virus was localized in the ... See full document

... equation 1 are treated as arbitrary ...plasma virus level by one-half) ranging from ...T cells coexpressing CD45RA (naive CD4 T cells) or CD45RO (memory CD4 T cells), peripheral blood ... See full document

... In contrast to most type C virus infections, cultures of human immunodeficiency virus type 1 HIV-1-infected T cells tend to undergo the persistent synthesis of viral DNA, with moderate t[r] ... See full document

... for human b-globin were included as a control for ...50,000 cells/lane were estimated by comparing graded doses of ACH-2 DNA lysate; this cell line contains 1 proviral copy/cell ... See full document

... H9 cells is of functional relevance was provided by comparing the effects of cyclosporine on replication of the wild-type virus in the two cell ...Jurkat cells than in H9 ...Jurkat ... See full document

... (R5) human immunodeficiency virus type 1 (HIV-1) ...NSI/R5 replication from the inhibitory effects of high AOP-RANTES concentrations (<100 ...retroviral ... See full document

... the human anti-HIV-1 immunoglobulin reacted only weakly with Gag-Pol p160, it was difficult to determine reliably the magnitude of the observed stimulation of ...12A2 cells are equivalent to ... See full document

... therefore may provide an alternative molecular genetic ap- proach to HIV-1 disease. Overall, this study supports the idea that a modified Cre could provide a reagent for therapy against HIV-1 infection. ... See full document

... T cells are the primary sites of human immunodeficiency virus type 1 (HIV-1) replication in ...antigen-presenting cells (APCs), such as dendritic ... See full document

... HIV-1 replication by rIL-16, when added to freshly infected cells, is not mediated at the level of proviral DNA ...viral replication suggest either a competitive inhi- bition of HIV-1 ... See full document

... in multiple states within a ...GFP-expressing cells revealed that a subset of the bright point sources did move rapidly within the ...of 1 would represent complete ... See full document

... these cells because of the presence of the endogenous murine cyclin T1, but it was ver- ified by Western analysis in NIH 3T3 cells expressing HA- tagged cyclin T1 in cells prepared in parallel (data ... See full document

... the human T-cell leukemia virus (HTLV) Rex response element in place of the RRE, in the presence of the functionally analogous but unrelated HTLV Rex protein ... See full document