[PDF] Top 20 Mutational analysis of the conserved basic domain of human immunodeficiency virus tat protein.
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Mutational analysis of the conserved basic domain of human immunodeficiency virus tat protein.
... To address the role of the conserved basic domain of the tat protein in the trans-activation of HIV-1 LTR-specific gene expression, we used oligonucleotidedirected mutagenesis 35 to alte[r] ... See full document
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Structure-Based Mutational Analysis of the C-Terminal DNA-Binding Domain of Human Immunodeficiency Virus Type 1 Integrase: Critical Residues for Protein Oligomerization and DNA Binding
... Eluted protein samples (wild-type IN) were analyzed on a denaturing polyacrylamide gel at various retention times ...Mutant protein V260E was mainly misfolded (data not ...mutant protein L242A the IN ... See full document
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Inhibition of human immunodeficiency virus type 1 and type 2 Tat function by transdominant Tat protein localized to both the nucleus and cytoplasm.
... HIV-1 Tat protein and its functional domains (19, 28, 41, 57, 58) are shown in ...and basic domains of Tat. To evaluate the ability of the mutant Tat proteins to activate HIV gene ... See full document
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Mutational Analysis of Conserved Domains within the Cytoplasmic Tail of gp41 from Human Immunodeficiency Virus Type 1: Effects on Glycoprotein Incorporation and Infectivity
... in conserved regions of the cytoplasmic domain of gp41 affect the incorporation of glycoprotein into virus par- ticles in infected H9 ...by virus particles pro- duced in transfected 293-T ... See full document
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Protein B23 is an important human factor for the nucleolar localization of the human immunodeficiency virus protein Tat.
... the basic region of Tat with its NoLS- binding ...mutant Tat-1 or Tat-2 ...wild-type Tat-1 and Tat-2 fusion proteins bound to B23 present in HeLa cell nuclear extract, while GST ... See full document
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Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domain.
... simian virus 40 nuclear localization signal (PKKKRKV) at the N terminus, to generate pCMV-nucl-ATG-TAP208–260; the correct orientation was established by PCR analysis with primers 18 (upstream) and 8 ...of ... See full document
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Mutational analysis of the conserved cysteine-rich region of the human immunodeficiency virus type 1 Tat protein.
... pHIV-1CATori is a pUC19-based plasmid containing the SV40 origin of replication HindIllSphI; SV40 nucleotides 5171 to 128 and the XhoI-BamHI fragment encoding the HIV-1 LTR chloramphenic[r] ... See full document
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Arginine Methylation of the Human Immunodeficiency Virus Type 1 Tat Protein by PRMT6 Negatively Affects Tat Interactions with both Cyclin T1 and the Tat Transactivation Region
... The Tat residues R52 and R53 are the targets of ...the Tat ARM, more specif- ically the 49-RKKRR-53 stretch, to participate in high-affinity binding for HIV TAR RNA ...wild-type Tat ARM with an ... See full document
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Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein
... that Tat interacts with the preinitiation complex through binding to p300, together with which it can mediate several ...vant, Tat-responsive RNA polymerase II complex at the pro- ...to Tat, though ... See full document
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Mutational Analysis of Residues in the Coiled-Coil Domain of Human Immunodeficiency Virus Type 1 Transmembrane Protein gp41
... The heptad repeat segment of gp41 is one of the most highly conserved regions in Env and plays a critical role in HIV entry. In this report, we analyzed the effects of substitution in resi- dues in the heptad ... See full document
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Effects of the Tat basic domain on human immunodeficiency virus type 1 transactivation, using chemically synthesized Tat protein and Tat peptides.
... 3343-3353 0022-538X/94/$04.0 + Copyright © 1994, American Society for Microbiology Effects of the Tat Basic Domain on Human Immunodeficiency Virus Type 1 Transactivation, Using Chemicall[r] ... See full document
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Mutational Analysis of the Human Immunodeficiency Virus Type 1 Vif Protein
... example, human immunodeficiency virus type 1 (HIV-1) encodes six accessory/regulatory genes in addition to the struc- tural and enzymatic gag, pol, and env genes that are present in all ... See full document
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Activation of transcription factor NF-kappaB by the Tat protein of human immunodeficiency virus type 1.
... fusion protein (Tat 2E) (see Results) will be presented ...fusion protein was allowed to bind to the beads at 48C on a rotating wheel for 1 ...fusion protein from contaminating bacterial ... See full document
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Increased expression of CD4 molecules on Jurkat cells mediated by human immunodeficiency virus tat protein.
... Our findings suggest that the single expression of the human immunodeficiency virus tat protein in the absence of the other viral proteins causes an upregulation of CD4 gene expression o[r] ... See full document
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Genetic Analyses of Conserved Residues in the Carboxyl-Terminal Domain of Human Immunodeficiency Virus Type 1 Integrase
... cated in binding to DNA. Amino acid side chains were highlighted on the dimeric NMR structure (Protein Data Bank entry 1IHV) (56) using the following coloring scheme: Val-234, red; Glu-246, orange; Lys-258, ... See full document
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Mutational Analysis of Conserved Amino Acids in the Influenza A Virus Nucleoprotein
... A virus consists of eight RNA ...highly conserved basic residues ...internal domain of NP. The architecture of RNP complexes in influenza A virus par- ticles is such that the RNPs are ... See full document
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Mutational Analysis of the N-Terminal Domain of Moloney Murine Leukemia Virus Capsid Protein
... and protein-protein interac- ...WT virus this clear space is formed upon complete proteolytic processing of Gag, allowing the CA protein to condense around the genome ...structurally ... See full document
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Functional comparison of the basic domains of the Tat proteins of human immunodeficiency virus types 1 and 2 in trans activation.
... Here we report the construction of chimeric Tatl and Tat2 substitution mutants expressing the basic domains of Tat2 and Tatl, respectively, and show that the basic domain of Tat2 contrib[r] ... See full document
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Molecular determinants for cellular uptake of Tat protein of human immunodeficiency virus type 1 in brain cells.
... of Tat on the host cell and hence effect ...cellular Tat causes neurotoxicity by acting on cell surface ex- citatory amino acid receptors (20) and that the neurotoxic epitope of Tat resides in the ... See full document
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Genetic analysis of the cofactor requirement for human immunodeficiency virus type 1 Tat function.
... As expected, analysis of the biological activity of these fusion proteins in transfected HeLa cells demonstrated that all four Tat missense mutations abrogated the ability of the Tat-Rev[r] ... See full document
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