[PDF] Top 20 Mutational Analysis of the Herpes Simplex Virus Type 1 UL25 DNA Packaging Protein Reveals Regions That Are Important after the Viral DNA Has Been Packaged
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Mutational Analysis of the Herpes Simplex Virus Type 1 UL25 DNA Packaging Protein Reveals Regions That Are Important after the Viral DNA Has Been Packaged
... The herpes simplex virus type 1 (HSV-1) UL25 gene encodes a minor capsid protein, pUL25, that is essential for packaging the full-length viral ...Six ... See full document
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The UL25 Gene Product of Herpes Simplex Virus Type 1 Is Involved in Uncoating of the Viral Genome
... Sequence analysis of the ts1204 and ts1208 ...HSV-1 DNA had mapped each of the mutations to a small region within UL25 ...These regions of the cloned wt HSV-1 DNA and the ... See full document
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Packaging of Genomic and Amplicon DNA by the Herpes Simplex Virus Type 1 UL25-Null Mutant KUL25NS
... amplicon DNA rel- atively more efficiently than it packages its own genome, (iv) the two termini of packaged amplicons are present in similar amounts, and (v) neither packaged viral genomes ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... blot analysis. Viral DNA replication generates concatemers that are cleaved into unit-length molecules and packaged into viri- ...cleaved viral DNA contains free chromosomal ter- ... See full document
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The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... The UL25 protein is associated with purified ...the UL25 protein is important for retaining cleaved DNA in capsids suggests that this protein may interact with capsids in ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the ...capsid-associated DNA-packaging proteins, UL17, UL25, and ... See full document
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Analysis of Herpes Simplex Virus Type 1 DNA Packaging Signal Mutations in the Context of the Viral Genome
... GC-rich regions, was tolerant of nucleotide ...generate virus indicates that DR1 also contains sequences essential for virus ...growth. Analysis of the deletion mutants identified only one ... See full document
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Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
... initiation has oc- curred, this vertex functions as the packaging channel and packaging is ...that 1 or 2 of the 12 vertices function as packaging vertices, since there may be ... See full document
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The Herpes Simplex Virus Type 1 DNA Packaging Protein UL17 Is a Virion Protein That Is Present in Both the Capsid and the Tegument Compartments
... infectious virus. In the absence of DNA packaging, the internal scaffolding proteins are still cleaved but remain trapped inside the angularized capsid, generating a dead-end product, the B ... See full document
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Herpes Simplex Virus DNA Packaging without Measurable DNA Synthesis
... measure packaging, Southern blotting and TCA precipitation, may indicate the fates of different populations of viral DNA ...older viral genomes, whereas South- ern blotting reveals the ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... stranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-length genomes by a complex mechanism that is tightly coupled to DNA insertion into a preformed capsid ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... null virus dlUL33 in transient assays and also failed to support cleavage and packaging of replicated amplicon DNA ...of viral DNA encapsidation and argue against its being ... See full document
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DNA-binding protein associated with herpes simplex virus DNA polymerase.
... Again, the protein was eluted by a gradient of 0 to 0.4 M KCI, and the fractions indicated by the solid horizontal line which were found to contain ICSP 34, 35 were pooled, dialyzed, and[r] ... See full document
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Effects of Mutations within the Herpes Simplex Virus Type 1 DNA Encapsidation Signal on Packaging Efficiency
... the herpes simplex virus type 1 genome lie within the terminally redundant region or a ...quasi-unique regions, Uc and Ub, occupy positions adjacent to the genomic L and S ... See full document
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Herpes simplex virus type 1 Angelotti and a defective viral genotype: analysis of genome structures and genetic relatedness by DNA-DNA reassociation kinetics.
... Standard virus stocks were prepared by passaging HSV ANG four 423 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest DNA-DNA reassociation kinetics of herpes simplex virus[r] ... See full document
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Packaging Determinants in the UL11 Tegument Protein of Herpes Simplex Virus Type 1
... UL11 packaging. To compare the relative levels of packaging for wt and mutant derivatives of UL11, a transfection/infection-based assay was ...of DNA per 100-mm plate for each con- struct) using ... See full document
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Physical map of the origin of defective DNA in herpes simplex virus type 1 DNA.
... The disappearance of the 5.4- to 5.7-Mdal EcoRI dDNA fragments after digestion with BamHI and the appearance of a new cluster of fragments, 2.6 to 3.2 Mdal, plus two 1-Mdal fragments B2 [r] ... See full document
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DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4
... (EMSA). DNA binding reactions and the subsequent gel shift analyses were performed as previously described (54) with slight ...with 1 ng of DNA probe in a buffer containing 10 mM HEPES- KOH (pH ... See full document
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
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DNA-negative temperature-sensitive mutants of herpes simplex virus type 1: patterns of viral DNA synthesis after temperature shift-up.
... For each gradient, 45 0.1-ml fractions were collected ISCO density gradient fractionator, model 640; Instrumentation Specialties Co., Lincoln, Neb., refractive in- 1043 Downloaded from h[r] ... See full document
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