[PDF] Top 20 Mutations in the gag gene of Moloney murine leukemia virus: effects on production of virions and reverse transcriptase.

... To explore the effects of these mutations on the utilization of the gag-pol precursor, we have introduced these mutants into cells already releasing defective particles from an endogenou[r] ... See full document

... proteins. Virions from the three replica- tion-delayed viruses (S526A, R657S, and Y598V) and the five noninfectious viruses ( D 5E, Y586F, D524N, H7, and D C) were isolated from culture medium of stable producer ... See full document

... mutant virus, however, was incapable of repli- ...mutant virus reverts to valine. The I597A mutant virus was able to give rise to spreading virus, but there was a very long delay, compared to ... See full document

... the gag, pol, and env genes, the HIV genome encodes a number of accessory proteins not found in MLV ...HIV gag and pol expression is dependent on the viral Rev protein, which facilitates transport of viral ... See full document

... Cells infected with virus of the two deletion mutants containing the largest deletions d11510 and d11524 did not themselves become producers of virus; no reverse transcriptase activity w[r] ... See full document

... ciency virus type 1 RT (HIV-1-RT), Y115, has been extensively studied by mutational analysis (5, 13, 16, ...the effects of these mutations are somewhat ...Y115 mutations in HIV-1-RT had a ... See full document

... Initially, we decided to assess the effects of monensin on the release of reverse transcriptase and p-gal activity from Psi2 cells expressing the Gag-p-gal GBG2051 protein, which is effi[r] ... See full document

... that effects on template switch- ing did not correlate with detectable defects in virus replication or gross defects in DNA ...in virus fitness should be magnified during repeated cycles of viral ... See full document

... The Gag proteins in the cell lysate and virions were immunoprecipitated and analyzed by electro- phoresis followed by fluorography ...of Gag at rates approximately half that of the wild ...wild-type ... See full document

... to effects on plus-strand ...leukosis virus and ASV is the presence of the host-derived src gene in its 39 UTR, it was previously believed that the 39 UTR might be a func- tionally dispensable, ... See full document

... The Gag protein of retroviruses plays a critical role in virion assembly (for reviews, see ...other virus-encoded components, this polyprotein alone is sufficient for inducing virus-like parti- cle ... See full document

... For mutations made at the AluI, EcoRV, 3489 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest The env gene products of Moloney murine leukemia virus are required for bindi[r] ... See full document

... To explore the cis-acting sequence requirements for the readthrough event in vivo, we generated a library of mutants of the Moloney murine leukemia virus with point mutations near the te[r] ... See full document

... The remaining ma42 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have measured reverse transcriptase enzyme activity per virus particle for samples of avian myelob[r] ... See full document

... XMRV virus expressing glyco-gag would show enhanced release through lipid ...XMRV Gag, giving the plasmid pMXMRV (Figure ...MXMRV virus showed expression of glyco-gag (the broad band of ... See full document

... The specificity of the rescue of HIV proteins into MuLV virions shows that specific interactions involving homologous matrix or capsid regions of Gag are necessary for retroviral particl[r] ... See full document

... Addition of plasmid DNAs containing the 5' portion of the mutant genome and the 3' portion of the wild-type line 1 gave rise to infectious virus with reverse transcriptase activity.. In [r] ... See full document

... 615 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have studied a mutant Moloney murine leukemia virus with a deletion in reverse transcriptase RT which is predicte[r] ... See full document

... the effects of the individual amino acid substitutions on the precise structure at the polymerase active site, the exact position of the template-primer, or ... See full document

... frameshift mutations (4, ...the mutations tested in the present study either had no effect or decreased the frequency of RT template ...affinity. Mutations in the primer grip have been pre- viously ... See full document