[PDF] Top 20 Neurons differentially control expression of a herpes simplex virus type 1 immediate-early promoter in transgenic mice.

... The immediate-early proteins of herpes simplex virus control the cascade of viral gene expression during lytic ...proteins control the reactivation of the viral ... See full document

... of virus reactivation. Additionally, two other mice sacrificed at 90 dpi were found positive for ICP0 ISH in the TG neurons, with a pattern similar to that observed at 28 dpi ...and early ... See full document

... gene expression and the selective transcription of the LATs are ...made early after infection (30, 33, ...using virus mutants in animal models of latency point to a major early decision, at ... See full document

... Herpes simplex virus type 1 (HSV-1) establishes a latent infection in peripheral sensory ganglionic neurons in humans and in the mouse ...the virus to naive ...in ... See full document

... of expression and/or the combination of these IFN-upregulated isoforms is necessary for long-term cell ...Input virus had been titrated to result in infection of approximately 90% of cells as assayed by ... See full document

... lanes 1 and ...a control to ensure that the lacZ and npt coding sequences were repressed in an equivalent manner, untransformed Vero cells were coin- fected with in1331 and in1321 and additionally ... See full document

... The immediate-early protein ICP0 of herpes simplex virus type 1 (HSV-1) plays an important regulatory role during viral infection in cultured cells and in mouse ... See full document

... The expression of herpes simplex virus (HSV) genomes in the absence of viral regulatory proteins in sensory neurons is poorly ...HSV immediate early (IE) mutant (d109) ... See full document

... ICER expression plasmid corre- sponded to decreasing luminescence values in a dose-depen- dent ...ICER expression plasmid, it was neces- sary to confirm that ICER specifically repressed the LAT pro- moter ... See full document

... study herpes simplex virus (HSV) latency establishment, maintenance, and ...gene expression in neurons following infection with replication-defective HSV recombinants carrying ␤ ... See full document

... ICP4 promoter was activated in large numbers of trigeminal ganglion neurons of newborn ICP4-␤-galactosidase transgenic mice (Tg6305) ...ICP0-␤-galactosidase transgenic mice ... See full document

... cellular control U3 snRNA promoter by dividing the “% input (IP-noab)” value for the viral DNA by that of the cellular DNA to account for the differences in immunoprecipitation ... See full document

... When considering molecular interactions involved in her- pesvirus tegument assembly, the tegument is often referred to as having an inner and outer compartment. This designation is based on the ease with which tegument ... See full document

... d3-4 ICP27 fails to modulate ICP0/4 localization in trans- fected cells. Transfection experiments have shown that ICP27 can promote the cytoplasmic localization of ICP0 and ICP4 even in uninfected cells (26, 68, 70). ... See full document

... mutant virus genotype is as ...1.5 expression from cells infected with R7815 was not compared to that from WT-infected cells, it was com- pared side by side with U S ... See full document

... Multiple cellular chromatin-remodeling factors and histone- modifying enzymes are recruited to HSV-1 replication compart- ments (RCs) (13), which are nuclear domains where viral DNA replication and late gene ... See full document

... Analysis of crossover points in the recombinant Bxl28, using restriction endonucleases EcoRI, Bgl II, Hpa I, HindIII plus Hpa I, and Kpn I plus Xba L HSV-1 physical maps are shown above,[r] ... See full document

... HOWELL,' LISA PETTERA,1 SHAHRAM TEHRANI,1 AND CARLOS LOPEZ2 Department of Laboratory Medicine and Pathology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School,[r] ... See full document

... cortical neurons of fetuses and in the mesencephalic region of neonate brains, with more foci in female than male ...fetal neurons, where the viruses were molecularly reactivated after ...that ... See full document

... To determine whether any of the transactivation-deficient IE-0 mutants that we isolated displayed a dominant negative phenotype, Vero cells were cotransfected with equimolar amounts of a[r] ... See full document