[PDF] Top 20 Non contiguous-finished genome sequence and description of Bacillus timonensis sp. nov.

... B. timonensis is susceptible to penicillin G, amoxicillin, vanco- mycin, gentamicin, erythromycin, doxycyclin, ri- fampicin, and ciprofloxacin but resistant to trime- ...the genome is 37.30%. The 16S rRNA ... See full document

... Bacterial taxonomy has undergone many changes over recent years. The DNA-DNA hybridization and G+C content criteria, once considered as gold standards [1], were gradually replaced by gene sequencing. In particular, 16S ... See full document

... are non-endospore forming, and are ...and genome se- quences are deposited in Genbank and EMBL under accession numbers JN657218 and CAHD00000000, ...the genome is ... See full document

... The runs were performed overnight and then ana- lyzed on the cluster through the gsRunBrowser and gsAssembler_Roche. The global 176,029 passed filter sequences generated 56.24 Mb with a length average of 319 bp. These ... See full document

... their genome sequence, MALDI-TOF spectrum and main phenotypic char- acteristics (habitat, Gram-stain reaction, cultiva- tion conditions, cell wall structure and metabolic ... See full document

... Cells stain Gram-negative. Catalase, oxidase and arginine dihydrolase activities, as well as esculin hydrolysis are present. Nitrate reduction and indole production are absent. Cells are susceptible to ticarcillin, ... See full document

... This latter value was lower than the 98.7% 16S rRNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization [40]. Different growth ... See full document

... The genome of C. massiliensis was compared with those of C. intestinalis, C. aerofaciens, C. stercoris, C. tanakaei, Eggerthella lenta and Coriobacterium glomerans (Table 6). The draft genome of C. ... See full document

... other type strains within the genus Anaerococcus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum- likelihood method ... See full document

... Open Reading Frames (ORFs) were predicted using Prodigal [22] with default parameters but the pre- dicted ORFs were excluded if they were spanning a sequencing GAP region. The predicted bacterial pro- tein sequences were ... See full document

... stored at -20°C until further use. The library was clonally amplified in 2 emPCR reactions at 0.25, 0.5 and 1 cpb with the GS Titanium SV emPCR Kit (Lib- L) v2 (Roche). The yield of the 3 types of paired- end emPCR ... See full document

... sidered as new species if they exhibit <96.0% and <95.4% sequence identity with other validly pub- lished species for the 327- and 825-bp fragments of the gltA and rpoB genes, respectively [2,11-13]. This ... See full document

... Colonies were smooth and transparent with 0.5 to 1 mm in diameter on blood-enriched Columbia agar. Optimal growth is only achieved anaerobi- cally and grows between 30 and 45°C, with opti- mal growth observed at 37°C. ... See full document

... 3,735,762 bp long (1 chromosome, but no plas- mid) with a 27,98% G + C content of (Figure 6 and Table 4). Of the 3,916 predicted genes, 3,843 pro- tein-coding genes, and 73 were RNAs. Eight rRNA genes (one 16S rRNA, one ... See full document

... complete genome sequence and its annotation. The 2,010,844 bp-long genome con- tains 1,909 protein-coding and 46 RNA genes, including two rRNA ... See full document

... Table 4). Of the 1,783 predicted genes, 1,738 were protein-coding genes, and 45 were RNAs. Three rRNA genes (one 16S rRNA, one 23S rRNA and one 5S rRNA) and 42 predicted tRNA genes were iden- tified in the genome. ... See full document

... committee of the IFR48 (Marseille, France) were obtained under agreement 09-022). The fecal specimen was preserved at -80°C after collection and sent to Marseille. Strain JC8E (Table 1) was isolated in January 2011 by ... See full document

... This value was lower than the 98.7% 16S rRNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization [2]. It should be noted that both A. ... See full document

... Open Reading Frames (ORFs) were predicted us- ing Prodigal [45] with default parameters. How- ever, the predicted ORFs were excluded if they spanned a sequencing gap region. The predicted bacterial protein sequences were ... See full document

... 4,616,135 bp long (1 chromosome, but no plas- mid) with a 34.10% G + C content (Figure 6 and Table 4). Of the 4,519 predicted genes, 4,432 were protein-coding genes, and 87 were RNAs. Eight rRNA genes (one 16S rRNA, one ... See full document