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[PDF] Top 20 Nonintegrating Foamy Virus Vectors

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Nonintegrating Foamy Virus Vectors

Nonintegrating Foamy Virus Vectors

... We also tested if the NIFV-MscvCre vector could remove a floxed transgene in gene-targeted mesenchymal stem cells (MSCs). These MSCs were obtained from an individual with osteogenesis imperfecta (OI) due to a ... See full document

9

Characterization of a cis-Acting Sequence in the pol Region Required To Transfer Human Foamy Virus Vectors

Characterization of a cis-Acting Sequence in the pol Region Required To Transfer Human Foamy Virus Vectors

... FIG. 4. Effect of CAS II on HFV protein expression and virion RNA content. The Gag-positive and Pol-negative vectors pMH5/M54, pMH38, and pMH40 were used to determine these effects. (A) The vector plasmids were ... See full document

8

Identification of Domains in Gag Important for Prototypic Foamy Virus Egress

Identification of Domains in Gag Important for Prototypic Foamy Virus Egress

... of virus particle budding but not in Gag synthesis or particle ...of virus particles to bud from the cell, or budding of normal quantities of less infectious ...PFV vectors were analyzed by SDS-PAGE ... See full document

8

Foamy Virus Bet Proteins Function as Novel Inhibitors of the APOBEC3 Family of Innate Antiretroviral Defense Factors

Foamy Virus Bet Proteins Function as Novel Inhibitors of the APOBEC3 Family of Innate Antiretroviral Defense Factors

... based vectors, we now demonstrate that PFV infectivity is strongly inhibited by the expression in producer cells of hA3G, hA3F, mA3, and, to a lesser extent, cpzA3G, but not by human APOBEC3A ...simian ... See full document

8

Persistent Infection with Primate Foamy Virus Type 1 Increases Human Immunodeficiency Virus Type 1 Cell Binding via a Bet-Independent Mechanism

Persistent Infection with Primate Foamy Virus Type 1 Increases Human Immunodeficiency Virus Type 1 Cell Binding via a Bet-Independent Mechanism

... and Bet 17. (A) Western-blot analysis of H9 clones, transduced with Bet-expressing (Bet 11 and Bet 17) or empty (Neo) MLV-based retroviral vectors, was performed as described in the legend to Fig. 1D; the pSGBet ... See full document

6

Further Characterization of Equine Foamy Virus Reveals Unusual Features among the Foamy Viruses

Further Characterization of Equine Foamy Virus Reveals Unusual Features among the Foamy Viruses

... expression vectors coding for the main viral products and the generation of specific antibodies against the main EFV proteins allowed us to determine the molecular sizes and to determine the subcellular ... See full document

8

Intra- and Intercellular Trafficking of the Foamy Virus Auxiliary Bet Protein

Intra- and Intercellular Trafficking of the Foamy Virus Auxiliary Bet Protein

... FIG. 5. Inter- and intracellular trafficking of EFV Bet pro- tein.(A) Schematic representation of the 3⬘ end of the EFV provirus. A 297-bp splice generates the Bet mRNA. 40 aa, 40 first residues shared with Tas. (B) ... See full document

7

Accuracy estimation of foamy virus genome copying

Accuracy estimation of foamy virus genome copying

... both vectors and packaged them with either PFV or SFVmac helper ...two vectors was packaged with high efficiency by its homologous Gag Pol proteins in the pres- ence of the competing heterologous ... See full document

15

Replacement of feline foamy virus bet by feline immunodeficiency virus vif yields replicative virus with novel vaccine candidate potential

Replacement of feline foamy virus bet by feline immunodeficiency virus vif yields replicative virus with novel vaccine candidate potential

... ficiency virus (FIV) have been used to develop vectors for vaccine antigen delivery and gene therapy in a vari- ety of mammals ...feline foamy virus (FFV) and FIV establish lifelong infec- ... See full document

21

Heparan Sulfate Is an Attachment Factor for Foamy Virus Entry

Heparan Sulfate Is an Attachment Factor for Foamy Virus Entry

... tion-incompetent foamy virus vector particles (MD9, described in reference 17) expressing EGFP as a reporter ...gene. Virus particles were pseudotyped with the envelope protein from ...PFV. ... See full document

8

Sequences in pol Are Required for Transfer of Human  Foamy Virus-Based Vectors

Sequences in pol Are Required for Transfer of Human Foamy Virus-Based Vectors

... of vectors based on replication-compe- tent and replication-incompetent HFV to deliver heterologous genes has been reported (8, 18, 38, ...these vectors still encode large parts of viral sequences, limiting ... See full document

7

Improved Primate Foamy Virus Vectors and Packaging Constructs

Improved Primate Foamy Virus Vectors and Packaging Constructs

... There is increasing interest in gene transfer using vectors derived from foamy viruses (FVs) (19, 43). In particular, their nonpathogenic nature, even in cases of accidental human in- fection, is a major ... See full document

10

Foamy virus vectors.

Foamy virus vectors.

... helper virus titers, while transfection with pFGPSN and pFdelGP produced nearly equivalent vector and helper titers after 7 days ...replication-competent virus, while pFdelGP can only produce helper ... See full document

6

Measles Virus Glycoprotein-Pseudotyped Lentiviral Vectors Are Highly Superior to Vesicular Stomatitis Virus G Pseudotypes for Genetic Modification of Monocyte-Derived Dendritic Cells

Measles Virus Glycoprotein-Pseudotyped Lentiviral Vectors Are Highly Superior to Vesicular Stomatitis Virus G Pseudotypes for Genetic Modification of Monocyte-Derived Dendritic Cells

... tropic MV genotypes) and VSV-G-LVs in RPMI 1640 without FCS in a flat-bottom 96-well plate for 2 h at 37°C at the indicated MOIs. The transduced MDDCs were then washed twice with RPMI 1640 and seeded in RPMI 1640 –10% ... See full document

12

Active foamy virus proteinase is essential for virus infectivity but not for formation of a Pol polyprotein.

Active foamy virus proteinase is essential for virus infectivity but not for formation of a Pol polyprotein.

... of virus particles harvested after transient transfection of BHK21 or BHK FAB cells (34) with plasmids pHSRV13, pHSRV-D/A, and pHSRV-S/T, respectively (Table ...infectious virus particles were recovered in ... See full document

5

Subviral Particle Release Determinants of Prototype Foamy Virus

Subviral Particle Release Determinants of Prototype Foamy Virus

... 1D, SVP release analysis normalized for cellular expression levels revealed the following: i partial or complete deletion of the CyD of the TM subunit ⌬C981 and ⌬C975 in the wild-type ba[r] ... See full document

12

Nuclear localization of foamy virus Gag precursor protein.

Nuclear localization of foamy virus Gag precursor protein.

... SCHLIEPHAKE AND AXEL RETHWILM* Institut fair Virologie und Immunbiologie, Wwrzburg, Germany Received 1 January 1994/Accepted 27 April 1994 cence that is observed when human immunodeficie[r] ... See full document

9

Replication of Simian Foamy Virus in Monkey Kidney Cells

Replication of Simian Foamy Virus in Monkey Kidney Cells

... The two types of infected cells differed in that the cytoplasm of MK cells usually contained 358 Downloaded from http://jvi.asm.org/ on November 11, 2019 by guest The structure of foamy [r] ... See full document

5

Insights into the structure and activity of prototype foamy virus RNase H

Insights into the structure and activity of prototype foamy virus RNase H

... deficiency virus type 1 (HIV-1) is enzymatically inactive [18-21], whereas that from Moloney murine leukemia virus (MoMLV) retains RNase H activity, albeit consid- erably lower than that of the full length ... See full document

11

Molecular Characterization of Proteolytic Processing of the Pol Proteins of Human Foamy Virus Reveals Novel Features of the Viral Protease

Molecular Characterization of Proteolytic Processing of the Pol Proteins of Human Foamy Virus Reveals Novel Features of the Viral Protease

... Human foamy virus (HFV) is the prototypic spumavirus that has distinct features of gene expression different from those of other known ...retroviruses. Foamy viruses (FVs) as complex retroviruses ... See full document

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