[PDF] Top 20 Optimization of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis for Bacterial Identification
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Optimization of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis for Bacterial Identification
... Additional factors could lead to unreliable identifications with MALDI-TOF MS, including database composition and depth. There is a demonstrated positive relationship between increased numbers of database spectra and ... See full document
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... turer, identification scores of ⱖ 2 and between ...reliable identification to the species and genus level, respectively, while identification scores of ⬍ ...API identification kits (bioMe ... See full document
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... The combined UW/Bruker and SARAMIS databases reliably differentiated M. tuberculosis from NTM species with their respec- tive extraction procedures. As has been previously reported (18, 19, 21), M. abscessus and M. ... See full document
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Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... nitrogen laser. The spectra were re- corded in the linear mode in a mass range of 2 to 20 kDa and subsequently analyzed using MALDI Biotyper Automation Control and Biotyper ...the time, the database ... See full document
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Microbial Typing by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?
... microbial mass spec- trum, we similarly propose that an average difference of 15 peaks could be tolerated ...all bacterial species since some show a substan- tially lesser share of variable peaks than ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
... cluster analysis with reference and chal- lenge isolates based on a pairwise correlation matrix was per- formed for both the ...cluster analysis mostly, but not fully, resolved ... See full document
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Fatal Nocardia farcinica Bacteremia Diagnosed by Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry in a Patient with Myelodysplastic Syndrome Treated with Corticosteroids
... Nocardia farcinica is a Gram-positive actinomycete sapro- phyte, ubiquitous in the environment. It is considered an opportunistic pathogen that affects patients with impaired cell-mediated immunity. Most of the patients ... See full document
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Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... Table 1 lists the measured masses of 23 plasma proteins that were available as puri- fied components, plus serum amyloid A, which was observed in serum from a patient with an acute phase response as characterized in ... See full document
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Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... subjected to MALDI-TOF MS analysis for identification at the species level. According to the spectral scores recommended by the manufacturer, 38 (40.9%) and 78 (83.9%) isolates were correctly identified at ... See full document
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Evaluation of the Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi
... mold identification has been the breadth of commercially available ...mold identification (11, ...the identification of filamentous fungi have used different MALDI-TOF MS systems, databases (both ... See full document
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... reliable identification. Phenotypic identification of Francisella species is inappropriate for clinical microbiology laboratories because it is time-consuming, hazardous and subject to variable ... See full document
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Identification of Molds by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... Molecular methods (e.g., DNA sequencing) are currently the gold standard for the identification of fungi to the species level. Although these methods can provide accurate results, they are expensive, require specialized ... See full document
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Identification of Mycobacteria by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... of matrix was overlaid on each sample well and allowed to dry for 15 ...The matrix used was a saturated solution of ␣-cyano-4-hydroxycinnamic acid (Sigma) dissolved in 1:1:1 acetonitrile, water, and ... See full document
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A case report of a cystic fibrosis patient with repeated isolation of Trichosporon mycotoxinivorans identified by a novel short extraction method
... 2012, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was performed for the first time from colony ... See full document
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Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel
... Commercial and extended databases for MALDI-TOF MS analysis. Two versions of the standard commercially available Bruker Daltonics database (BDAL) were used in this study (see Table S2 in the supplemental ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... the identification of pathogens from blood cultures, these will vary because of a number of other factors, such as the exact distribution of typical pathogens isolated, the utilization of MALDI-TOF MS and ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... difficulty of assessing some measures. The cost savings did not account for indirect cost savings from decreased wastes, quality control of replaced tests, and the training and labor associated with these retired ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... Statistical analysis of staphylococcal distribution between pe- riods 1 and 2 revealed that MALDI-TOF MS allowed a signifi- cantly higher rate of identification to be determined for ...available ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... The panel of chemotaxonomic tests was performed, and meso- DAP was detected in whole-cell hydrolysates, as were arabinose and galactose as diagnostic sugars. In addition, glucose and man- nose were identified. ... See full document
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Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in ...However, matrix-assisted laser ... See full document
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