[PDF] Top 20 Origin of Expression of the Herpes Simplex Virus Type 1 Protein US1.5

... as protein detected in WT transfected/infected cells ...of protein) as increasing amounts of the N terminus of ICP22 were ...in expression of proteins that comigrated with those detected in cells ... See full document

... the origin binding activity of other viral replication initiator proteins, we sought to determine whether the phosphorylation state of OBP affects its ability to form protein-DNA complexes at its ... See full document

... in protein expression levels, a transient transfection-immunoblot experiment was ...R387K expression plasmid, and expression was induced by hr94 ...cell protein extracts were resolved ... See full document

... (pCMVOBPC 1 ; Fig. 1) or OBP (pCMVUL9; Fig. 1) under the control of the human cytomeg- alovirus (HCMV) immediate-early ...of 1:100 ...OBPC expression in transfected cells or less ... See full document

... regulatory protein of herpes simplex type 1 (HSV-1) lytic infection is the 63-kDa nuclear phosphoprotein IE63 (also known as ...late virus gene expression ...host ... See full document

... RESULTS Our standard assay for HSV origin-dependent DNA replication used the following plasmids: pKl-2, encoding the immediate-early protein IE175; pMC122 XbaI-F, which contains dbp, pol[r] ... See full document

... least 5-fold greater spectral counts in HSV1-GFPVP26 compared to the HSV1- GFP control immuno-isolations were considered high confidence candidate interactions (Supplementary table ...only 1 - 3 tryptic ... See full document

... SCMV and HCMV proteinases can cleave the HSV-1 pAP at its M-site. Plasmids encoding both the short (i.e., LM8, LM12, and EV2) and long (i.e., AW4, LM13, and EV1) forms of the SCMV, HCMV, and HSV-1 ... See full document

... To characterize more thoroughly the kinetics with which the 65KDBP gene is expressed, we have examined not only the accumulation of 6SKDBP mRNA and protein, as compared to other well-cha[r] ... See full document

... Targeting information is not found within the first 10 resi- dues of UL11. If the acidic cluster is required only for recovery from the plasma membrane, then what in the first half of UL11 is responsible for ... See full document

... fusion protein and its incorporation into the virus ...the virus replication cycle ...(Fig. 5). Early in infection (6 and 8 h; Fig. 5A and B) the fusion protein localized to distinct ... See full document

... Wt virus-infected BHK21 cells were subjected to centrifugation on a sucrose gradient, and fractions were collected across the gradient and analyzed by SDS-PAGE and then by silver ...ment protein VP16, which ... See full document

... for 5 min (at 4°C) and resuspended by mild vortexing in ...Tris, 1 mM EDTA [pH ...Twelve 1-ml fractions were collected from the bottom of the tube for analysis by flu- ... See full document

... GFP expression vectors (2 to 20 ␮g of DNA per 100-mm plate for each con- struct) using Lipofectamine 2000 ...equal expression levels, each vector was titrated prior to the start of the ...the ... See full document

... Construction of pGFP-VP22 plasmid. pGFP- VP22 was constructed by in frame ligation of BamH1-Xba1 fragment of pVP22/myc-His2 (Invitrogen, Carlsbad, CA, USA), a commercially available VP22 expression vector that ... See full document

... probes with a sequence located between the PCR primers for UL50 (5 ⬘ -GGAG GATGCCGGTTT-3⬘), UL51 (5⬘-CCGCACGTACCACGCC-3⬘), and UL52 (5⬘- GGTTCTGTTGCACGAG-3 ⬘ ) were synthesized by PE Applied ... See full document

... (AcUL33 virus) or mutated (Acin14 to Acin116) forms of UL33 and with AcUL28Myc, expressing a c-Myc-tagged form of ...lanes 1 to 3) demonstrating that precipitation of UL28-Myc by R148 is specifically ... See full document

... ate virus stocks for superinfection studies, mutant dl1403Y, which is dl1403 with an insertion of YFP controlled by the HCMV MIEP at the TK locus, was ...nonpenetrated virus, and viral-DNA levels were ... See full document

... nucleotide 1 (14), and at the EcoRI site that occurs in the linker insertion in these ...dues 1 to 163, was joined to the C-terminal portion of mutant S13 from residues 264 to ...residues 1 to ... See full document

... with 1 ng of DNA probe in a buffer containing 10 mM HEPES- KOH (pH 7.9), 5 mM ammonium sulfate, 8% (vol/vol) glycerol, 2% (wt/vol) polyethylene glycol 8000, 80 mM KCl, 5 mM ␤ -mercaptoethanol, ...and ... See full document