[PDF] Top 20 Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
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Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
... pRB143 containing the BamHI S fragment into the XmaI site of ...GST-ORF P fusion protein was inoculated into two ...ORF P 11 , the repaired virus, or R7528 and main- tained at 378C were ... See full document
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Neither LAT nor Open Reading Frame P Mutations Increase Expression of Spliced or Intron-Containing ICP0 Transcripts in Mouse Ganglia Latently Infected with Herpes Simplex Virus
... ORF P, which conceivably could be encoded by a minor LAT species as well as by L/STs, has been hypothesized to repress ICP0 expression during latency by inhibition of ICP0 splicing ...wild-type virus, (ii) ... See full document
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The U(L)15 gene of herpes simplex virus type 1 contains within its second exon a novel open reading frame that is translated in frame with the U(L)15 gene product.
... The herpes simplex virus type 1 (HSV-1) genome contains two components, long (L) and short (S), that are covalently linked and that contain unique sequences (U) flanked by in- verted ... See full document
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Up to Four Distinct Polypeptides Are Produced from the γ34.5 Open Reading Frame of Herpes Simplex Virus 2
... 34.5 gene was similar to what had been previously reported in the GenBank database (accession number ...and 1 Pro ...construct containing a Myc tag at the original stop codon in the intron ... See full document
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Herpes Simplex Virus 1 Open Reading Frames O and P Are Not Necessary for Establishment of Latent Infection in Mice
... of virus on scarified corneas as described ...in 1 ml of 199V containing nystatin, and incubated for 5 ...per virus were removed, flash frozen, and stored at ...in 1 ml of ... See full document
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The Herpes Simplex Virus Type 1 UL3 Transcript Starts within the UL3 Open Reading Frame and Encodes a 224-Amino-Acid Protein
... recombinant virus CB8116 at an MOI of ...line 1. The left ends of the unique long region (UL) of HSV-1 strains 17 ⫹ and F and recombinant viruses derived from strain F are shown expanded in lines 2 ... See full document
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A Virus with a Mutation in the ICP4-Binding Site in the L/ST Promoter of Herpes Simplex Virus Type 1, but Not a Virus with a Mutation in Open Reading Frame P, Exhibits Cell-Type-Specific Expression of γ134.5 Transcripts and Latency-Associated Transcripts
... The herpes simplex virus type 1 L/S junction-spanning transcripts (L/STs) are a family of multisized tran- scripts expressed at high levels in cells infected with mutant viruses that (i) do ... See full document
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Herpes Simplex Virus ICP27 Is Required for Virus-Induced Stabilization of the ARE-Containing IEX-1 mRNA Encoded by the Human IER3 Gene
... vhs open reading frame present in the ...(Strata- gene), generating pUC8 vhs Xba2 ...vhs open reading frame for eGFP coding ...vhs open reading ... See full document
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Transcription of the derepressed open reading frame P of herpes simplex virus 1 precludes the expression of the antisense gamma(1)34.5 gene and may account for the attenuation of the mutant virus.
... tk gene of R7545 was repaired by plating the progeny of the cotransfection of rabbit skin cells with R7545 DNA and pRB103, which contains the BamHI Q fragment, on 143TK 2 cells overlaid in Dulbecco modified Eagle ... See full document
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The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.
... ORF P protein are as ...ORF P is expressed in the absence of prior protein synthesis, consistent with the presence of an a TIF response element closely matching the consensus se- quence described by Mackem ... See full document
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... for several serine proteases (29, 30). Each substrate phage in the library carries phagemid sequence encoding a fusion pro- tein consisting of truncated M13 phage gene III protein and a random pentapeptide fused ... See full document
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The transcriptional activation domain of varicella-zoster virus open reading frame 62 protein is not conserved with its herpes simplex virus homolog.
... Plasmid GALA contains the DNA binding domain amino acids 1 to 147 [18] of the yeast GAL4 protein preceded by the simian virus 40 early promoter and followed by a polylinker sequence 29..[r] ... See full document
6
Fine mapping of the latency-related gene of herpes simplex virus type 1: alternative splicing produces distinct latency-related RNAs containing open reading frames.
... 32P-labeled genomic DNA restriction fragments and synthetic oligonucleotides were used as probes for in situ hybridizations and Northern RNA blot hybridizations of RNA from trigeminal ga[r] ... See full document
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Phenotypic Suppression of a Herpes Simplex Virus 1 ICP27 Mutation by Enhanced Transcription of the Mutant Gene
... In this study, we set out to isolate growth-competent rever- tants of dLeu. We first picked eight dLeu plaques on ICP27- complementing V27 cells (18). These viruses were amplified once in V27 cells and then serially ... See full document
6
Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27.
... 1987 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Varicella-zoster virus VZV open reading frame 4 ORF4 encodes a putative immediate-early protein which is homologous [r] ... See full document
6
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene.
... exposed to 10 PFU of wild-type or mutant virus per cell and harvested at the times indicated in Results. For infections in the presence of cycloheximide, 100 m g of drug (Sigma) per ml of medium was present both ... See full document
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Varicella-zoster virus open reading frame 4 encodes a transcriptional activator that is functionally distinct from that of herpes simplex virus homology ICP27.
... First, it is possible that the upstream elements merely function to boost the basal transcriptional activity of the promoter and that activation by the ORF4 gene product is not discerned[r] ... See full document
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Extensive homology between the herpes simplex virus type 2 glycoprotein F gene and the herpes simplex virus type 1 glycoprotein C gene.
... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document
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Herpes Simplex Virus 1 and 2
... of virus into susceptible mucosal surfaces (eg, oropharynx, cervix, conjunctiva) or through small cracks in the ...The virus is readily inactivated at room temperature and by drying; hence, aerosol and ... See full document
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Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1.
... SCOLNICK'* Laboratory of Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland 20205,' and Department of Microbiology, University of Chicago, Chicago, Illinois 606372 We co[r] ... See full document
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