[PDF] Top 20 Pluripotent stem cell differentiation as an emerging model to study human prostate development

Pluripotent stem cell differentiation as an emerging model to study human prostate development

... the human prostate and rodent prostate, such as differ- ences in histology and ...of human prostate materials, especially embryonic specimens, a more widespread method is urgently re- ... See full document

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Abstract: Recent technical advances in the stem cell field have enabled the in vitro generation of

... 3D human gastric organoids through directed in vitro differentiation of hESCs and ...vivo human stomach development by inducing first the PSCs to form definitive endoderm spheroids, which were ... See full document

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Fluorescent gene reporters in human pluripotent stem cells : as model for studying human heart development and cardiomyocyte differentiation

... the cell membrane and are able to monitor membrane potential, based on fluctuating fluorescent ...the cell through activation of L-type voltage-gated Ca2+ ... See full document

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Modulating the biochemical and biophysical culture environment to enhance osteogenic differentiation and maturation of human pluripotent stem cell-derived mesenchymal progenitors

... the development of high-throughput TE strategies on a common platform (for instance, by designing stem cell culture environments in micro-bioreactor formats) ...of cell and culture materials ... See full document

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Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells

... that human iPSCs can differentiate into ...this study, we answered the two questions and confirmed that DNA demethylation accelerated the induction of PGCs from iPSCs in ...induction differentiation ... See full document

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p53 switches off pluripotency on differentiation

... that human adult cells were also capable of being converted into pluripotent stem cells under similar conditions [2, ...any cell type, human iPSCs have the potential to replace damaged ... See full document

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Human pluripotent stem cells: an emerging model in developmental biology

... the development of the human ...numerous human diseases caused by developmental ...the human genome, physiology and ...embryonic development is ...manipulate human ... See full document

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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes

... vitro model to study ...drug development to circumvent the lack of sufficient and healthy human car- diac tissue ...into cell index values [16]. In the current study, hESC-and ... See full document

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A Pluripotent Stem Cell Model of Human Placental Development.

... hESC cell line (H9) that expresses an inducible shRNA against YAP ...This cell line along with a scrambled shRNA control were the kind gift from ...abolished differentiation to EVT and STB, as ... See full document

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Understanding Megakaryopoiesis And Thrombopoiesis Using Human Stem Cells Models

... Human stem cell models (CD34+ hematopoietic progenitors, embryonic stem cells and induced pluripotent stem cells (iPSCs)) are powerful tools for the study of ... See full document

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In Pursuit of Porcine Pluripotent Stem Cells for Autologous Cell Therapy

... vitro differentiation of presumptive porcine iPSC into rod photoreceptors and their integration into the retinas of recipient pigs has been reported ...of stem cell-based treatment to recover the ... See full document

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Pluripotent Human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy

... of cell survivability after infection with EV71, with and without pre-treatment with SP81 peptide Cell survivability after exposure to the SP81 peptide and EV71, were assessed with the CellTiter-Blue™ ... See full document

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Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions

... activated cell sort- ing (FACS) is customarily used in a laboratory setting for cell separation based on the expression of specific surface antigens [78–80] or fluorescent reporters [81] (after genetic ... See full document

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Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor

... Iran. Human adipose–derived stem cells (hADSCs) were isolated from three 45 ± 3 old years women after a written consent had been obtained, using a standard cell culture protocol as previously ... See full document

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Conserved two-step regulatory mechanism of human epithelial differentiation

... early prostate SC ...progenitor cell marker CD49f (Figures 3J and 3K) and a 12% decrease in CD49b expression (data not shown); (3) a decrease in CK5 (basal CK) expression with a concomitant increase in ... See full document

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Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

... high cell attachment was achieved on these polymers, suggesting that only small amounts of HPhMA are required to encourage cell ...increase cell attachment over ... See full document

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Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs

... induced pluripotent stem cells (iPSCs) that generated by reprogramming adult cells with defined factors (c-MYC, OCT4, SOX2, KLF4) might provide another promising source of immune-matched autologous cells ... See full document

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Wnt/β catenin signaling directs the regional expansion of first and second heart field derived ventricular cardiomyocytes

... SHF early ventricular CMs from embryos and corresponding ESC lines are controlled by the β-catenin signaling pathway uncovers a mechanistic pathway for the control of in vitro cardiogenesis and in vivo adult heart size. ... See full document

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Friedreich's ataxia induced pluripotent stem cell derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency

... Intracellular Ca 2+ was measured by microfluorimetry (Ionoptix MA, USA). Cells were loaded with the Ca 2+ fluorescent dye FURA-2/AM (5 ȝM, 10 min incubation at room temperature, Molecular Probes). They were placed in a ... See full document

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Human endogenous retrovirus K (HML 2) RNA and protein expression is a marker for human embryonic and induced pluripotent stem cells

... certain human specific, protein encoding HERV-K(HML-2) proviruses with promoter competent LTR sequences in pluripotent ESCs, iPSCs, and ...all cell lines tested may indi- cate a particular function ... See full document

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