[PDF] Top 20 Posttranslational modification and subcellular localization of the p12 capsid protein of herpes simplex virus type 1.
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Posttranslational modification and subcellular localization of the p12 capsid protein of herpes simplex virus type 1.
... In contrast, the TrpE-UL35 antiserum reacted with two proteins, designated pl2a and pl2b, which were present in lysates of HSV-infected cells radiolabeled with either [35S]methionine or [r] ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... mutant virus is unable to cleave and package viral DNA, consistent with the phenotype of a previously isolated temperature-sensitive herpes simplex virus type 1 mutant, tsN20 ... See full document
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Herpes Simplex Virus Type 1 Glycoprotein E Is Required for Axonal Localization of Capsid, Tegument, and Membrane Glycoproteins
... collected. Protein content was determined by the BCA assay (Pierce), and equal concentrations were loaded onto a 4 to 15% SDS- PAGE ...NS-gEnull virus infection of the ... See full document
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The herpes simplex virus 1 protein kinase encoded by the US3 gene mediates posttranslational modification of the phosphoprotein encoded by the UL34 gene.
... 3B show that i in the infected cell lysates of R7306, the phosphoprotein profile was that of the wild-type HSV-1F and ii the phosphoprotein profile of the lysates of cells infected with [r] ... See full document
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Herpes Simplex Virus 1 Protein Kinase Us3 and Major Tegument Protein UL47 Reciprocally Regulate Their Subcellular Localization in Infected Cells
... GTP-binding protein Ran (21, 22). Phosphorylation of a protein near its NLS, mediated by many different protein kinases, is a key and common mechanism by which the transport of NLS-containing ... See full document
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ATP-Dependent Localization of the Herpes Simplex Virus Capsid Protein VP26 to Sites of Procapsid Maturation
... The herpes simplex virus type 1 (HSV-1) capsid shell is composed of four major polypeptides, VP5, VP19c, VP23, and ...the capsid hexons formed by ...fluorescent ... See full document
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Distinctions between Bovine Herpesvirus 1 and Herpes Simplex Virus Type 1 VP22 Tegument Protein Subcellular Associations
... Fluorescence microscopy of the VP22 homologs fused to GFP transfected into cell lines revealed fascinating contrasts between BVP22 and HVP22. Although both homologs could associate with host microtubules, reorganizing ... See full document
12
Posttranslational Processing of Infected Cell Proteins 0 and 4 of Herpes Simplex Virus 1 Is Sequential and Reflects the Subcellular Compartment in Which the Proteins Localize
... wild-type virus and treated with roscovitine, a specific inhibitor of cell cycle-dependent kinases cdc2, cdk2, and ...immunoprecipitated protein was reduced and resistant to roscovitine and may ... See full document
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The Cyclin-Dependent Kinase Inhibitor Roscovitine Inhibits the Transactivating Activity and Alters the Posttranslational Modification of Herpes Simplex Virus Type 1 ICP0
... of posttranslational modifications af- fected by Rosco do not involve phosphorylation and that the phosphorylated peptides whose analysis is shown in ...this protein in Vero ...other ... See full document
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Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Localization in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1
... in virus envelope and/or tegument assembly have not yet been ...and capsid proteins in the juxtanuclear cytoplasmic domain and accumulation of virus particles in the compartment have been ...of ... See full document
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Identification of a minimal hydrophobic domain in the herpes simplex virus type 1 scaffolding protein which is required for interaction with the major capsid protein.
... HSV capsid assembly is that ICP35 and the C-terminal domain of the protease share identical amino acid sequences (28, ...and posttranslational phosphorylations ...scaffolding protein ICP35 (33, 34). ... See full document
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Herpes Simplex Virus Capsid Localization to ESCRT-VPS4 Complexes in the Presence and Absence of the Large Tegument Protein UL36p
... HSV capsid-associated and Vps4-EQ-GFP-bound cytoplasmic ...numbered 1 and 2) are shown as higher-magnification ...C), 1 m (insets in panel C), and ... See full document
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Subcellular Localization of Herpes Simplex Virus Type 1 UL51 Protein and Role of Palmitoylation in Golgi Apparatus Targeting
... protein’s subcellular localization, conformation, protein-protein interactions, and activity (50, 61, 64, ...UL51 protein can take two different forms, a membrane-binding form and a ... See full document
13
Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... the subcellular localiza- tion of UL24 was to assemble clues to the function of UL24 during ...virion-associated protein involved in viral transport from the nucleus to the cell membrane (1, ...cell ... See full document
8
Localization of Herpes Simplex Virus Type 1 UL37 in the Golgi Complex Requires UL36 but Not Capsid Structures
... matrix protein) and mannosidase II (a Golgi resident protein) but not with GM130 (a vesicle-tethering protein lo- infected with K37eGFP/26R at an MOI of 10 PFU/cell and the cells visualized live with ... See full document
8
Incorporation of the Green Fluorescent Protein into the Herpes Simplex Virus Type 1 Capsid
... the localization of the VP26- GFP fusion protein and its incorporation into the virus ...the virus replication cycle ...fusion protein localized to distinct regions within the nucleus ... See full document
6
Eclipse Phase of Herpes Simplex Virus Type 1 Infection: Efficient Dynein-Mediated Capsid Transport without the Small Capsid Protein VP26
... 3f and h) and gB (not shown). Thus, these particles either represented vesicles containing viral membrane proteins or virions with an intact membrane which prevented an internal contrasting of the capsids. The latter ... See full document
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Isolation of a protein kinase induced by herpes simplex virus type 1.
... The pooled individual peaks were 383 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have isolated a new cyclic AMP-independent protein kinase activity induced in He[r] ... See full document
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Herpes simplex virus type 1 ICP27 is an essential regulatory protein.
... We report herein the genetic and phenotypic characterization of four temperature-sensitive mutants of herpes simplex virus type 1 tsY46, tsE5, tsE6, and tsLG4 that have begun to elucidat[r] ... See full document
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Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... a protein referred to as a replication ...simian virus 40 (SV40), whose ability to initiate viral DNA replication is both positively and negatively regulated by phosphorylation (reviewed in reference ...by ... See full document
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