[PDF] Top 20 Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
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Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
... latter samples were retested with the standard con- sensus PCR test using MY09/MY11/HMB01 from a different oligonucleotide synthesis lot and with the line blot test using biotin-labeled MY09/MY11/HMB01 as ... See full document
6
International Proficiency Study of a Consensus L1 PCR Assay for the Detection and Typing of Human Papillomavirus DNA: Evaluation of Accuracy and Intralaboratory and Interlaboratory Agreement
... The PGMY L1 consensus primer pair combined with the line blot assay allows the detection of 27 genital human papillomavirus (HPV) ...of PCR for HPV DNA ... See full document
7
Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma
... the consensus degenerate primers (L1 gene) [MY09: 5 ’ -GCM CAG GGW CAT AAY AAT GG-3 ’ , MY11:5 ’ -CGT CCM ARR GGA WAC TGA TC-3 ’ where M = A/C; W = A/T; Y = C/T; R = A/G) with an amplicon size 450 ... See full document
10
Use of multiple PCR primer sets for optimal detection of human papillomavirus
... Consensus PCR products were detected by hybridization to consensus ...and detection of fluorescein-labelled consensus amplimer ...Gp primers were used to amplify HPV type 11, 16, ... See full document
6
Analysis of the Effect of DNA Purification on Detection of Human Papillomavirus in Oral Rinse Samples by PCR
... Human papillomavirus (HPV) has recently been associated with oral ...the DNA purification method on HPV genomic DNA detection in Scope mouthwash oral rinse samples and the ... See full document
10
Detection of human papillomavirus DNA in cervical lavage specimens by a nonisotopic consensus PCR assay
... for DNA-RNA hybrids. As described previ- ously (12), the PCR products generated with consensus primers were reacted separately in a solution hybridization reaction with each ... See full document
6
Nonisotopic Detection and Typing of Human Papillomavirus DNA in Genital Samples by the Line Blot Assay
... combines PCR with nonisotopic detection of amplified DNA, was evaluated for its ability to detect human papillomavirus (HPV) DNA in genital ...Processed samples ... See full document
6
Detection of genital human papillomavirus by single tube nested PCR and type specific oligonucleotide hybridization
... sensitive detection system. The use of PCR for HPV detection provides several advantages over the classical South- ern blotting technique ...used PCR-based systems for detection ... See full document
7
Detection of Human Papillomavirus Type 16 DNA in Consecutive Genital Samples Does Not Always Represent Persistent Infection as Determined by Molecular Variant Analysis
... Persistent human papillomavirus (HPV) infection of the uterine cervix is a risk factor for progression to high-grade squamous intraepithelial ...lesions. Detection in consecutive genital ... See full document
6
Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single Hybridization, Reverse Line Blot Detection Method
... biotin-labeled PCR product hybridized to an array of immobilized oligonucleotide ...swab samples collected in Digene specimen transport medium (Digene Diag- nostics, Silver Spring, ...two detection ... See full document
8
Comparison of Human Papillomavirus Detection and Typing by Cycle Sequencing, Line Blotting, and Hybrid Capture
... HPV PCR assay and cycle sequencing. HPV DNA was amplified by using L1 consensus primers MY11 and MY09 ...(4). DNA quantity and integrity were monitored through amplification of ... See full document
5
Enhanced Detection and Typing of Human Papillomavirus (HPV) DNA in Anogenital Samples with PGMY Primers and the Linear Array HPV Genotyping Test
... HPV DNA detection and HPV ...HPV DNA and individual types compared with ...using PGMY-LB will have to assess the opportunity to use LA-HPV because of the greater sensitivity of ...some ... See full document
9
Nonisotopic Detection of Human Papillomavirus DNA in Clinical Specimens Using a Consensus PCR and a Generic Probe Mix in an Enzyme Linked Immunosorbent Assay Format
... 282 samples) of results in this particular study. Seven of the 15 samples yielding border- line results contained HPV DNA detectable using the reverse line blot, including three specimens containing ... See full document
7
MassARRAY Spectrometry Is More Sensitive than PreTect HPV Proofer and Consensus PCR for Type Specific Detection of High Risk Oncogenic Human Papillomavirus Genotypes in Cervical Cancer
... all samples were tested in quadruplicate to rule out any preanalytical contamination or experimental artifacts, and negative controls were satisfactory in all runs, thus ruling out false-positive ...reactions. ... See full document
8
Detection of human Papillomavirus 18 in cervical cancer samples using PCR-ELISA (DIAPOPS)
... For detection of HPV in paraffin-embedded specimens, the 5-µm sections were cut and deparaffinized with ...the samples and incubated overnight at ...extracted. DNA was precipitated by the addition of ... See full document
6
A novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples
... continued use [4]. Several other serologically based assays are available for use in typhoid diagnosis including Typhidot and Tubex [5,6], but have the same problems associated with the use of the ... See full document
7
Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... Tinea (ringworm) is an infection of keratinized tissues (epi- dermis, hair, and nails) by a group of specialized fungi, the dermatophytes. Dermatophytes belong to three anamorphic genera, Epidermophyton, Microsporum, and ... See full document
6
Rapid detection of human papillomavirus in cervical scrapes by combined general primer mediated and type specific polymerase chain reaction
... A two-step polymerase chain reaction PCR procedure was used as a new screening strategy for the detection of human papillomavirus HPV genotypes in cervical scrapes omitting prior DNA ext[r] ... See full document
5
Forest diseases : FTA cards and DNA sampling
... Appendix 5.3 Continued PCR amplification of the Acacia mangium root seedlings samples using ITS primers in different DNA extraction methods PCR Results 1 Samples.. DNA Extraction Method.[r] ... See full document
201
Molecular epidemiology of Human Papillomaviruses in breast cancer, Golestan province of Iran
... and human papilomavirus: epidemiological evidence and perspectives for ...of papillomavirus sequences in condylomatous lesions of the mamillae and in invasive carcinoma of the ... See full document
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