[PDF] Top 20 Processing of the Semliki Forest virus structural polyprotein: role of the capsid protease.
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Processing of the Semliki Forest virus structural polyprotein: role of the capsid protease.
... We showed directly that the protease activity responsible for the release of the capsid protein resides in the capsid itself: by progressive truncation of the cDNA used for the SP6 trans[r] ... See full document
9
M-X-I Motif of Semliki Forest Virus Capsid Protein Affects Nucleocapsid Assembly
... as described above. SDS-PAGE analysis of cleared cell lysates showed correct protein production and processing compared to the wild type (Fig. 5). Particles released into the media from cells transfected with the ... See full document
8
Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.
... the structural capsid proteins and the nonstructural proteins ...picornaviral polyprotein is processed cotranslationally by a proteolytic cascade, result- ing in a number of smaller structural ... See full document
6
Structural plasticity of the Semliki Forest virus glycome upon interspecies transmission
... biosynthetic processing of N-linked ...For virus propagation and puri fi cation, BHK and mosquito C6/ 36 cells were infected with ...the capsid protein (Figure ... See full document
12
Properly Folded Nonstructural Polyprotein Directs the Semliki Forest Virus Replication Complex to the Endosomal Compartment
... individual Semliki Forest virus (SFV) nonstructural proteins nsP1 to nsP4 in different combinations, as well as their precursor polyprotein P1234 and its cleavage ...the processing of ... See full document
12
Molecular Determinants of Substrate Specificity for Semliki Forest Virus Nonstructural Protease
... cysteine protease domain of Semliki Forest virus nonstructural protein 2 (nsP2) regulates the virus life cycle by sequentially cleaving at three specific sites within the ... See full document
10
Furin Processing and Proteolytic Activation of Semliki Forest Virus
... alphavirus Semliki Forest virus (SFV) infects cells via a low-pH-dependent membrane fusion reaction mediated by the E1 envelope ...furin-like protease. Once processing to E2 occurs, the ... See full document
9
Polyprotein Processing Protease of African Swine Fever Virus: Purification and Biochemical Characterization
... fever virus (ASFV) is the causative agent of a severe hemorrhagic disease of domestic ...icosahedral virus that infects macro- phages/monocytes of swine as well as ticks of the Ornithodoros ...the ... See full document
5
Differences in Processing Determinants of Nonstructural Polyprotein and in the Sequence of Nonstructural Protein 3 Affect Neurovirulence of Semliki Forest Virus
... negative-strand RNA (12). Additionally, nsP1 is involved in the induction of type I interferon (6, 13, 14). nsP2 is a multidomain protein. The N-terminal half of nsP2 contains domains required for NTPase and RNA ... See full document
16
Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of the capsid protein from the polyprotein
... the structural components of the viral matrix, i.e. capsid and nucleocapsidproteins; the pol domains gener- ally comprise the protease, reverse transcriptase, ribonu- clease and an ... See full document
12
trans-Protease Activity and Structural Insights into the Active Form of the Alphavirus Capsid Protease
... alphavirus capsid protease is an attractive antiviral therapeutic ...FRET-based protease assay for screen- ing capsid protease ...Our structural studies unveil the ... See full document
12
Polyprotein processing in cis and in trans by hepatitis A virus 3C protease cloned and expressed in Escherichia coli.
... 5242 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To determine the P3 region protein-processing sites cleaved by the hepatitis A virus 3C protease, a nested set of co[r] ... See full document
6
Proteolytic processing in African swine fever virus: evidence for a new structural polyprotein, pp62.
... major structural proteins as polyproteins could serve to cluster genes whose products are somehow related, allowing them to be synthesized as the result of a single transcription-translation event and, if ... See full document
6
Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor.
... Thus, the production of all three membrane proteins is directed by alternating signal and stop-transfer anchor sequences that function in translocation and cleavage of the virus precurso[r] ... See full document
8
Specific in vitro cleavage of a Leishmania virus capsid-RNA-dependent RNA polymerase polyprotein by a host cysteine-like protease.
... the capsid gene, possibly via in- ternal translation initiation as shown for Hv190S ...dsRNA virus L-A (7) and Giardia virus (37), both of which express their RDRP genes as a Cap-Pol fusion protein ... See full document
8
Nucleotide sequence at the junction between the nonstructural and the structural genes of the semliki forest virus genome.
... An examination of the nucleotide sequences upstream from the initiator codon for the structural proteins on the 42S RNA genome shows that all reading frames are effectively blocked by st[r] ... See full document
5
Studies on defective interfering Semliki Forest virus
... DI virus from any natural virus infection, either of man or any other ...DI virus is clinically isolated, its role in the modulation of virus infections must be treated with ...the ... See full document
295
Processing of gag precursor polyprotein of human T-cell leukemia virus type I by virus-encoded protease.
... Symbols: 0, 7.5K gene promoter of vaccinia virus; izz, gag coding sequence of HTLV-I; , protease-coding sequence; = , reverse transcriptase coding of HTLV-I; V, insertion of 8-nucleotide[r] ... See full document
11
Role of the Amphipathic Peptide of Semliki Forest Virus Replicase Protein nsP1 in Membrane Association and Virus Replication
... decisive role in the membrane binding of the replication com- plex, since the other three replicase proteins cannot directly associate with membranes ...for virus viability ...primary role of the ... See full document
12
Karyophilic properties of Semliki Forest virus nucleocapsid protein.
... Low concentrations of highly purified native C protein 103 to 104 molecules per cell transferred into various target cells induce the synthesis of specific host cellular proteins 10.. Hi[r] ... See full document
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