[PDF] Top 20 The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
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The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential ...the UL25 gene ... See full document
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Characterization of Pseudorabies Virus (PrV) Cleavage-Encapsidation Proteins and Functional Complementation of PrV pUL32 by the Homologous Protein of Herpes Simplex Virus Type 1
... for cleavage and encapsidation of viral ...of viral DNA similar to those in cells infected with PrV-Ka were found ...not required for DNA ...digested DNA from ... See full document
14
The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids
... the UL25 gene product of herpes simplex virus (HSV) have been identified in all three subfamilies of the ...during viral replication is not yet ...the UL25 protein ... See full document
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The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.
... the cleavage of concatameric replicated viral DNA prior to its packaging into ...of viral polypeptides which play a role in this encapsidation process have been identified ... See full document
9
Proteolytic Cleavage of the Amino Terminus of the UL15 Gene Product of Herpes Simplex Virus Type 1 Is Coupled with Maturation of Viral DNA into Unit-Length Genomes
... proposed cleavage site ...the cleavage may be mediated by a protease like the signal peptidases which demonstrate flexibility in their respec- tive recognition sequences ...different virus- or ... See full document
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Novel Class of Thiourea Compounds That Inhibit Herpes Simplex Virus Type 1 DNA Cleavage and Encapsidation: Resistance Maps to the UL6 Gene
... UL6 gene products from HSV-1 and HSV-2 is ...an encapsidation complex. These interactions can influence the encapsidation process at various levels: (i) the correct subcellular localization of ... See full document
8
Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
... The herpes simplex virus 1 (HSV-1) virion consists of a 152-kbp double-stranded DNA (dsDNA) genome that is en- closed in an icosahedral capsid, which is itself surrounded by an ... See full document
13
Packaging of Genomic and Amplicon DNA by the Herpes Simplex Virus Type 1 UL25-Null Mutant KUL25NS
... packaged replicated amplicon DNA rela- tively more efficiently than it packaged its own genome and in amounts approaching that encapsidated in the presence of wt HSV-1 ...genomic DNA, however, ... See full document
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The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores
... 11 viral glycoproteins that protrude externally and a small number of intrinsic membrane proteins ...23 viral proteins but also contains cellular proteins as well as viral and cellular gene ... See full document
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Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.
... nuclease gene of herpes simplex virus type 1 (HSV-1) (nuc mutations) induce almost wild-type levels of viral DNA; however, mutant viral yields ... See full document
11
Mutational Analysis of the Herpes Simplex Virus Type 1 UL25 DNA Packaging Protein Reveals Regions That Are Important after the Viral DNA Has Been Packaged
... of UL25 mutant plasmids. Regions of UL25 were mutated either by PCR, using pFBpCI-UL25 as the template and primers containing the mu- tations, or by annealing overlapping oligonucleotides possessing ... See full document
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Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
... packaging DNA is similar to that of double-stranded DNA (dsDNA) bacteriophages such as T4, P22, and lambda (9, 27, 28, ...in UL25 null mutant- infected cells results from an abortive packaging event ... See full document
10
Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.
... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document
11
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... the DNA packaging process. In wild-type virus-infected cells, empty capsids are only rarely enveloped (35), and a variety of viral mutants that synthesize capsids but fail to package ... See full document
13
The UL25 Gene Product of Herpes Simplex Virus Type 1 Is Involved in Uncoating of the Viral Genome
... HSV-1 DNA had mapped each of the mutations to a small region within UL25 ...HSV-1 DNA and the corresponding portions in the cloned mutant DNAs were se- quenced ...ts1204 UL25 ... See full document
13
Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... HSV-1 UL25 protein is one of seven viral proteins that are required for DNA cleavage and ...packaging. UL25 is found at or near each of the vertices of ... See full document
11
Effects of Mutations within the Herpes Simplex Virus Type 1 DNA Encapsidation Signal on Packaging Efficiency
... herpesvirus cleavage packaging signals has employed two principal ...a viral origin of DNA replication and putative packag- ing signal (so-called amplicons) are transfected into tissue cul- ture ... See full document
10
The Herpes Simplex Virus Type 1 UL17 Gene Encodes Virion Tegument Proteins That Are Required for Cleavage and Packaging of Viral DNA
... During herpes simplex virus type 1 (HSV-1) infection, indi- vidual viral genomes are cleaved from intranuclear con- catameric viral DNA and packaged into ... See full document
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Pocket Protein p130/Rb2 Is Required for Efficient Herpes Simplex Virus Type 1 Gene Expression and Viral Replication
... of 1⫻ PBS. Cells were pelleted through 1 ml of CS and then resuspended in 200 l of 1⫻ PBS with ...and viral DNA were extracted using the QIAamp DNA minikit (Qiagen) according to ... See full document
12
Two regions of the herpes simplex virus type 1 UL42 protein are required for its functional interaction with the viral DNA polymerase.
... Although a UL42 mutant RNA encoding a product lacking the 28 C-terminal residues resulted in wildtype levels of pol stimulation, the failure of large aminoterminal N-terminal and C-termi[r] ... See full document
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