[PDF] Top 20 The 1:1 N-NS protein complex of vesicular stomatitis virus is essential for efficient genome replication.
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The 1:1 N-NS protein complex of vesicular stomatitis virus is essential for efficient genome replication.
... To determine whether the effect of lower pH on genome RNA replication and nucleocapsid assembly was due to defective soluble protein, we performed a series of reconstitution reactions wi[r] ... See full document
6
Expression and purification of vesicular stomatitis virus N-P complex from Escherichia coli: role in genome RNA transcription and replication in vitro.
... soluble N-P complex in large amounts free from eucary- otic cellular proteins for in vitro ...of N protein alone in bacteria makes it com- pletely insoluble and that high-salt (1 M ... See full document
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Regulation of RNA Synthesis by the Genomic Termini of Vesicular Stomatitis Virus: Identification of Distinct Sequences Essential for Transcription but Not Replication
... of vesicular stomatitis virus (VSV), a nonsegmented negative-strand RNA virus, directs two discrete RNA synthetic processes, transcription and ...contain essential signals for these ... See full document
10
Importance of Hydrogen Bond Contacts between the N Protein and RNA Genome of Vesicular Stomatitis Virus in Encapsidation and RNA Synthesis
... quasi-helix 1 and are located on two regions positioned between the N and C-terminal lobes that are postulated to be “hinge” regions ...the N protein suggested they might play a functional ... See full document
11
Study of the Assembly of Vesicular Stomatitis Virus N Protein: Role of the P Protein
... purified N protein complex containing no P pro- tein was determined to be ...the N protein does not bind mRNA during a viral infection ...P protein, the N protein ... See full document
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Evaluating Replication-Defective Vesicular Stomatitis Virus as a Vaccine Vehicle
... C virus structural proteins (VSV ⌬ ...Vaccinia virus encoding the T7 polymerase (vTF7-3) was used to infect these “helper” BHK cells 24 h later (MOI of 10) followed by transfection with pVSV ⌬ G or pVSV ⌬ ... See full document
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Overlapping Signals for Transcription and Replication at the 3′ Terminus of the Vesicular Stomatitis Virus Genome
... delta virus ribozyme sequences, respectively; le9 and tr9 represent complementary sequences of leader and trailer regions of the VSV genome; N and DL represent the coding sequences of the N ... See full document
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Structural Properties of the C Terminus of Vesicular Stomatitis Virus N Protein Dictate N-RNA Complex Assembly, Encapsidation, and RNA Synthesis
... the N proteins of the following members of the family Rhabdo- viridae were aligned using MAFFT software: VSV-IND (VSIV) (NBCI database accession ...Chandipura virus (CHPV) (AAU81954), Isfahan virus ... See full document
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Constitutive phosphorylation of the vesicular stomatitis virus P protein modulates polymerase complex formation but is not essential for transcription or replication.
... polymerase complex and the P-N assembly complex, the vesicular stomatitis virus (VSV) P protein plays a pivotal role in transcription and replication of the viral ... See full document
11
A Polyamide Inhibits Replication of Vesicular Stomatitis Virus by Targeting RNA in the Nucleocapsid
... the genome. During transcription/replication, the vRdRp must access the structured RNA genome sequestered in the nucleocapsid and will not recognize the free RNA genome as a template for RNA ... See full document
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Identification of a Novel Tripartite Complex Involved in Replication of Vesicular Stomatitis Virus Genome RNA
... and N genes at a multiplicity of infection ratio of 1:5:5 ...Fig. 1. (A) A 20-g aliquot of total protein for each sample was analyzed in an SDS-polyacrylamide gel followed by Coomassie blue ... See full document
7
The Membrane-Proximal Stem Region of Vesicular Stomatitis Virus G Protein Confers Efficient Virus Assembly
... Although much progress has been made in defining domains that are important for the assembly and release of virions from the cell surface, as well as for glycoprotein incorporation into virions, relatively little is ... See full document
8
Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus.
... 15, 1975 VS VIRION TRANSCRIPTASE protein is dialyzed against 0.1 M NaCl column buffer; a mixture of 1 volume each of template and L protein, 2 volumes of NS protein, and 4 volumes of pol[r] ... See full document
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Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus.
... d Purified template and solubilized proteins prepared from other virus samples were recombined under saturating conditions in a standard binding assay.. Downloaded from http://jvi.asm.or[r] ... See full document
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Specific interactions of vesicular stomatitis virus L and NS proteins with heterologous genome ribonucleoprotein template lead to mRNA synthesis in vitro.
... Since interaction of N-RNA template with the soluble transcriptase results in RNA synthesis, heterologous in vitro transcription reconstitution systems offer an excellent system to study[r] ... See full document
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Vesicular stomatitis virus NS proteins: structural similarity without extensive sequence homology.
... 60 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The complete nucleotide sequence of the NS mRNA of vesicular stomatitis virus New Jersey serotype was established fro[r] ... See full document
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Protein Composition of the Structural Components of Vesicular Stomatitis Virus
... 3, 1969 VS VIRUS STRUCTURAL PROTEINS 617 CY, of each fraction were extracted with acetic acid, 0.5 mt urea, SDS, and 2-mercaptoethanol along with3 H-protein markers from intact purified [r] ... See full document
8
Role of matrix protein in cytopathogenesis of vesicular stomatitis virus.
... Second, we cloned cDNA copies of the entire coding region for M protein of wild-type virus and of a ts mutant into a simian virus 40 SV40 expression vector and compared the CPE in transf[r] ... See full document
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Phosphorylation of NS protein by vesicular stomatitis virus nucleocapsids: lack of effect during RNA synthesis and separation of kinase from L protein.
... MASSEY, NANCY DEANS, AND JOHN LENARD* Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Pisc[r] ... See full document
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Characterization of Vesicular Stomatitis Virus Pseudotypes Bearing Essential Entry Glycoproteins gB, gD, gH, and gL of Herpes Simplex Virus 1
... neutralizes virus infec- tivity, inhibits cell-cell spread (36), and blocks cell-cell fusion of cells transiently expressing the four entry glycoproteins ...an essential interaction between gH/gL and gB ... See full document
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