[PDF] Top 20 Proteolytic Processing of the Human Immunodeficiency Virus Envelope Glycoprotein Precursor Decreases Conformational Flexibility
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Proteolytic Processing of the Human Immunodeficiency Virus Envelope Glycoprotein Precursor Decreases Conformational Flexibility
... Env proteolytic cleavage allows fur- ther glutaraldehyde-mediated conformational fixation that does not occur efficiently in the context of the cleavage-deficient ...between proteolytic matura- tion ... See full document
6
Characterization of human immunodeficiency virus type 2 envelope glycoproteins: dimerization of the glycoprotein precursor during processing.
... Extracts from infected cells (panel CELL) and from culture medium containing virus particles (panel SN) were purified on HIV-2 serum-Sepharose to identify the viral glycoproteins gp125, [r] ... See full document
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Conformational Differences between Functional Human Immunodeficiency Virus Envelope Glycoprotein Trimers and Stabilized Soluble Trimers
... the conformational similarity of this cytoplasmic tail-deleted Env to full-length Envs (40, 85, ...Optimizing proteolytic cleavage could help to enrich the percentage of EnvΔ712 in a state 1 conformation ... See full document
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Mutations in Envelope gp120 Can Impact Proteolytic Processing of the gp160 Precursor and Thereby Affect Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Pseudoviruses
... 293T cells were transfected with each expression plasmid and tested for Env expression and the ability to mediate fusion. One variant failed to express Env, and two variants expressed Env but did not mediate fusion (data ... See full document
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Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins
... In virus-infected cells, the envelope glycoprotein (Env) precursor, gp160, of human immunodeficiency virus type 1 is cleaved by cellular proteases into a fusion-competent ... See full document
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Oligomeric and Conformational Properties of a Proteolytically Mature, Disulfide-Stabilized Human Immunodeficiency Virus Type 1 gp140 Envelope Glycoprotein
... the conformational freedom of the two ...mational flexibility, such that “freezing” of the conformation by CD4 binding results in an unexpectedly large loss in entropy ...recognize conformational ... See full document
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Modifications That Stabilize Human Immunodeficiency Virus Envelope Glycoprotein Trimers in Solution
... the envelope glycoproteins were secreted into the medium of expressing ...YU2 envelope glycoprotein was terminated after arginine ...YU2 envelope glycoprotein residues up to and ... See full document
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Analysis of the interaction of the human immunodeficiency virus type 1 gp120 envelope glycoprotein with the gp41 transmembrane glycoprotein.
... the conformational sen- sitivity and lower expected flexibility of these structures may contribute to their less efficient ...oligomeric envelope glycoprotein complex (85, ...gp120 ... See full document
10
Processing of the envelope glycoprotein gp160 in immunotoxin-resistant cell lines chronically infected with human immunodeficiency virus type 1.
... the human immunodeficiency virus (HIV) and resistant to the action of immunotoxins directed against the HIV envelope ...the envelope protein precursor ...truncated ... See full document
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Stabilization of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Trimers by Disulfide Bonds Introduced into the gp41 Glycoprotein Ectodomain
... gp160 glycoprotein. This glycoprotein was expressed efficiently on the cell surface and was precipitated by antibodies that recognize conformation-depen- dent gp120 epitopes (29, ...subtle ... See full document
6
Sulfation of the human immunodeficiency virus envelope glycoprotein.
... cells infected with the IIIB isolate of HIV-1 and in the cell lysates and culture media of cells infected with vaccinia virus recombinants expressing a full-length or truncated, secreted[r] ... See full document
7
Mutational analysis of the cleavage sequence of the human immunodeficiency virus type 1 envelope glycoprotein precursor gp160.
... Our results indicate that a glutamic acid to glycine change at gpl20 amino acid 516, a lysine to isoleucine change at amino acid 517, and an arginine to lysine change at amino acid 518 a[r] ... See full document
6
Identification and characterization of fusion and processing domains of the human immunodeficiency virus type 2 envelope glycoprotein.
... To characterize the domains of the HIV-2 envelope gly- coprotein that are involved in processing and fusion, single amino acid substitutions were introduced in three regions of the HIV-2[r] ... See full document
7
Fusion function of the Semliki Forest virus spike is activated by proteolytic cleavage of the envelope glycoprotein precursor p62.
... spike precursor protein activates the fusion function of the alphavirus spike similarly to the cleavage activation of fusion proteins in other virus families (as described above).. We ha[r] ... See full document
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Structure of the murine leukemia virus envelope glycoprotein precursor.
... Plates of NIH/3T3 cells infected with Moloney MuLV were pulse-labeled with J5S]methionine for 30 min (panel A), labeled (30 min) and chased in complete media for 30 min (panel B), or lab[r] ... See full document
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Determinants of human immunodeficiency virus type 1 envelope glycoprotein oligomeric structure.
... on glycoprotein fusion function, CD4 binding, and gp120-gp41 ...env glycoprotein fusion func- ...env glycoprotein induced syncytia with up to 20 to 50 nuclei, all deletion mutants failed to induce ... See full document
10
Comparison of Uncleaved and Mature Human Immunodeficiency Virus Membrane Envelope Glycoprotein Trimers
... on virus neutralization ...Thus, proteolytic maturation allows the membrane-anchored Env to achieve a conformation that retains functional metastability but masks epitopes for poorly neutralizing ... See full document
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Oligomeric Modeling and Electrostatic Analysis of the gp120 Envelope Glycoprotein of Human Immunodeficiency Virus
... The human immunodeficiency virus envelope glycoproteins, gp120 and gp41, function in cell entry by binding to CD4 and a chemokine receptor on the cell surface and orchestrating the direct ... See full document
12
Stoichiometry of Envelope Glycoprotein Trimers in the Entry of Human Immunodeficiency Virus Type 1
... The virus stocks, which are capable of a single round of infection, were harvested 2 days later, aliquoted, and stored at ⫺ ...mutant envelope glycoproteins were ...produce virus stocks with ... See full document
16
Modifications of the Human Immunodeficiency Virus Envelope Glycoprotein Enhance Immunogenicity for Genetic Immunization
... HIV envelope protein are likely to present different epitopes to the immune system compared to monomeric ...the envelope, this trimeric structure is likely to display conformational epitopes ... See full document
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