[PDF] Top 20 Pyrosequencing™ : A one step method for high resolution HLA typing
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Pyrosequencing™ : A one step method for high resolution HLA typing
... the high-resolution genotyping of Exon II of HLA-DRB1 (Wang et al, unpublished ...genotyping resolution by introduc- ing fewer numbers of out-of-phase dispensations (Wang et al, unpublished ... See full document
10
Development of a high-resolution NGS-based HLA-typing and analysis pipeline
... first step is a filtering step, which reduces the num- ber of possible ...second step the method tries to determine the most likely genotype of the ...has high unique start point ... See full document
8
Ultra-high resolution HLA genotyping and allele discovery by highly multiplexed cDNA amplicon pyrosequencing
... the high-quality read lengths were insufficient to provide complete coverage (data not ...of HLA- B*18:01:01:01 in sample HLA-Ref20 despite a primer mismatch for one of the ...of one ... See full document
14
Lyssavirus Detection and Typing Using Pyrosequencing
... the one-step RT-PCR in as- sociation with pyrosequencing presents the advantage of being a mutation-resistant method, since it is based on primers de- signed on conserved regions, while the ... See full document
7
Six-locus high resolution HLA haplotype frequencies derived from mixed-resolution DNA typing for the entire US donor registry
... of HLA haplotype frequency estimates depends on the typing method ...Early HLA typing based on serology had high levels of mistyping and therefore was not deemed ade- quate for ... See full document
8
Genotyping of Human Papillomaviruses by a Novel One Step Typing Method with Multiplex PCR and Clinical Applications
... hybridization step with HPV-specific probes and is rapid and ...a high sensitivity and a high degree of ...developed method, we conducted a pilot study to examine the correlation between the ... See full document
8
High Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing
... From one up to five samples were tested in geographically distinct trapping ...MST method may become a new genotyping method for the epidemiological surveillance of ... See full document
8
A rational two-step approach to KRAS mutation testing in colorectal cancer using high resolution melting analysis and pyrosequencing
... the high incidence of CRC resulting in high demand for KRAS mutation testing, an ideal diagnostic assay for this purpose not only needs to be sufficiently sensitive and specific, but, for socio-economic ... See full document
12
A glow of HLA typing in organ transplantation
... Sequencing-based typing (SBT) is a high-resolution method for the identification of HLA ...of HLA Class I alleles can be discriminated by their exon 2 and 3 sequence, and for ... See full document
5
Polymorphism in clinical immunology – From HLA typing to immunogenetic profiling
... of high-throughput sequencing or guide the production of high-density ...probes. One set includes overlapping oligos representing an arbitrarily selected "consensus" sequence (consensus- oligos), the ... See full document
11
Evaluation of High Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples
... too high, inducing too much resolution, the epidemiological links might get ...MLVA typing should especially be ana- lyzed with ...This method relies on determining the number of single ... See full document
10
HLAreporter: a tool for HLA typing from next generation sequencing data
... achieve high accuracy, data with good coverage on HLA genes are also ...and HLA calls (Figure S4 in Additional file ...missing one of the two alleles, calling homozygous on a heterozy- gous ... See full document
12
Methylation-Specific Differentiation of Vaginal Epithelial Cells for Forensic Tissue Typing by Bisulfite Conversion and Pyrosequencing
... test detects the actual presence of ɑ-amylase antigens as opposed to their activity against starch (Casey & Price, 2010). Semen Several tests exist for the presumptive identification of seminal fluid. One ... See full document
63
Fuzzy rock typing: Enhancing reservoir simulation and modeling by honoring high resolution geological models
... This method in which the permeability (kk r ) of each phase is scaled up (by solution of a Laplace equation for example) has appeared in several guises ...the method is the same as the capillary-equilibrium ... See full document
135
14/12/2012. HLA typing - problem #1. Applications for NGS. HLA typing - problem #1 HLA typing - problem #2
... © Zentrum für Humangenetik und Laboratoriumsmedizin Martinsried.. Routine HLA typing by Next Generation Sequencing.[r] ... See full document
6
Resolution of Ambiguous HLA Genotyping in Korean by Multi Group Specific Sequence Based Typing
... 10533 HLA alleles are registered in the IMGT/HLA database release ...more HLA alleles are identified, more ambiguity would arise from SBT, and the potential for ambiguous results in- creases as ... See full document
9
Core Genome Multilocus Sequence Typing Scheme for High Resolution Typing of Enterococcus faecium
... The German ST18 isolates UW5429 and UW6476 and the Danish ST80 isolate V13 and ST192 isolate V45 are relative outliers within their indicated clusters, differing by ⱖ 30, 28, 283, and 20 alleles, respectively, from any ... See full document
10
High-Throughput High-Resolution Class I HLA Genotyping in East Africa.
... Abstract HLA, the most genetically diverse loci in the human genome, play a crucial role in host-pathogen interaction by mediating innate and adaptive cellular immune ...the HLA genetic diversity in this ... See full document
18
HLA-DQA1 and HLA-DQB1 in Celiac disease predisposition: practical implications of the HLA molecular typing
... [32,33]. HLA class I heterodimers are constituted by an alpha-heavy chain, encoded by HLA-A, B or C loci, and by a small beta2- microglobulin molecule whose gene maps on chromosome ...15. HLA class ... See full document
5
Predictive Fluorescent Amplified Fragment Length Polymorphism Analysis of Escherichia coli: High Resolution Typing Method with Phylogenetic Significance
... FAFLP with an unlabelled nonselective EcoRI primer (Eco 1 0) and a labelled selective MseI primer (Mse 1 TA) gave strain-specific profiles. Fragments of identical sizes (in base pairs) were assumed to be identical, and ... See full document
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