[PDF] Top 20 Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
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Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... HBV DNA level were determined. HBV DNA density was determined by ...HBV DNA peaks are found, one at the density expected to contain the HBV Dane particle population ...HBV DNA-containing ... See full document
10
Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections
... our real-time NASBA assays demonstrated sev- eral advantages that could make it a valuable complement to traditional blood culture for the diagnosis of ...earlier detection of the gram-negative ... See full document
12
Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... the real-time multiplex NASBA is between 5 and 50 CCU, ...the real-time mono and multiplex NASBA reactions is for Legionella ...the real-time mono NASBAs were optimized for the ... See full document
7
Quantitative Detection of the M204V Hepatitis B Virus Minor Variants by Amplification Refractory Mutation System Real Time PCR Combined with Molecular Beacon Technology
... lecular beacon technology and clinical samples were compara- ble to those obtained with experimental ...laboratory-synthesized DNA target ...target sequence represented the minor ... See full document
7
Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... Several molecular techniques have been developed and validated for the diagnosis and management of HBV infection in the clinical time PCR technology based on Taqman ...HBV DNA with wider ... See full document
5
Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... Mycoplasma real-time PCR. Using criteria required for the design of molecular-beacon assays, primer and molecular-beacon sequences were selected from a P1 cytadhesin gene ... See full document
6
Anti hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion
... house nucleic acid tests (NAT) to directly detect the presence of HBV DNA using a combination of amplification and detection techniques similar to HCV RNA was employed ...HBV DNA ... See full document
7
Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... amplifiable DNA and to assess the effectiveness of the DNase treatments, the NA extracts were assayed by real-time PCR for the -globin gene (forward primer, 5⬘-CAG GTA CGG CTG TCA TCA CTT AGA-3⬘; ... See full document
6
Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification
... IQ5 Real-Time analyser ...for amplification (BioMerieux) according to the manufacturers instructions with a KCl concentration of 80 mM for ...l) molecular beacon (20 μM) and template ... See full document
6
Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real Time Nucleic Acid Sequence Based Amplification
... sensitive detection of Aspergillus from clinical samples may facilitate the early diagnosis of invasive pulmonary aspergillosis ...A real-time nucleic acid sequence-based ... See full document
6
Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia
... Two molecular beacons (Fig. 1a and b) were designed to distinguish between the two genetic subgroups of HIV-1 sub- type C (C and C⬘) cocirculating in ...NASBA amplification. Fig- ure 1a demonstrates ... See full document
8
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... techniques based on genomic sequence detection by reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and ... See full document
9
Nuclease resistant double stranded DNA controls or standards for hepatitis B virus nucleic acid amplification assays
... armored DNA by using an HBV DNA real-time PCR detection kit (Shang- hai KeHua Bio-engineering ...extracted DNA was used as a template for PCR ...are sequence-specific ... See full document
7
Rapid and sensitive real time assay for the detection of respiratory syncytial virus using RT SIBA®
... similar time-to-detection as RADTs, while retaining the high sensitivity achieved with ...diagnostic time, we developed an isothermal reverse transcription strand invasion based ... See full document
7
Nucleic Acid Sequence Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR
... is based on precipitation and may therefore be unsuitable for the iso- lation of small RNAs like the EBERs, which would precipitate less ...for detection of EBER transcripts after the silica-based ... See full document
6
High Throughput Detection of West Nile Virus RNA
... sensitive nucleic acid-based techniques, including nucleic acid se- quence-based amplification (NASBA) (6) and branched DNA (bDNA) methods, are available for ... See full document
8
Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
... nodes, bone marrow, liver or spleen and on culturing parasites from these sites. However, aspirate sampling is uncomfortable for the patient and the isolation of para- sites by culturing is time-consuming, ... See full document
6
Diagnosing Herpesvirus Infections by Real Time Amplification and Rapid Culture
... the real-time PCR assays was also tested by ap- plying the tests on cell cultures of other viruses that can be expected to be present in the samples that were ... See full document
5
Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays
... Plasmid DNA was isolated on the Vistra Labstation (Amersham Phar- macia Biotech, Roosendaal, The ...the real- time PCR detection assay, a standard curve of the plasmid ranging from 10 million ... See full document
5
Quantitation of Hepatitis B Virus Genomic DNA by Real Time Detection PCR
... serum virus levels frequently induce fluctuation of serum ALT ...serum virus level is useful in monitoring the clinical course of patients with active hepatitis ...reliable quantitative assay ... See full document
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