[PDF] Top 20 Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1 1)
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Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1 1)
... one assay originally de- veloped and already approved for use for the measurement of the viral load in HIV-1 carriers may be equally valid for the quantification of HIV-2 viremia is of ...the ... See full document
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Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... The plasma human immunodeficiency virus (HIV) RNA load is used in the clinical routine for the moni- toring of HIV infection and the patient’s response to antiretroviral ...HIV ... See full document
6
Plasma RNA Viral Load in Human Immunodeficiency Virus Type 2 Subtype A and Subtype B Infections
... the RNA of HIV-2 subtype A and subtype B strains in ...The detection limit is 250 copy equivalents/ml, and the reproducibility of the assay is ...the assay, as ... See full document
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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants
... of human immunodeficiency virus type 1 (HIV-1) RNA levels is important for understand- ing the natural history of HIV infection, predicting disease progression to AIDS, ... See full document
6
Multicenter Evaluation of the Performance Characteristics of the NucliSens HIV 1 QT Assay Used for Quantitation of Human Immunodeficiency Virus Type 1 RNA
... more RNA target per amplification ...the plasma input volume from 1 to 2 ml increased the sensitivity of the assay to 50 HIV-1 RNA copies/ml, with a detection rate ... See full document
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Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... of plasma HIV-1 RNA levels appears to hold many advantages over other virologic tests for HIV-1 infection ...antigen, plasma HIV-1 RNA levels are measurable in the vast ... See full document
5
Performance Characteristics of the QUANTIPLEX HIV 1 RNA 3 0 Assay for Detection and Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... HIV-1 RNA in ...and detection methods, including reverse tran- scription (RT) followed by PCR and colorimetric detection (RT-PCR assay; Roche Molecular Systems, ...orimetric ... See full document
9
Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... of 2 min at 50°C; 30 min at 60°C (for RT); 4 PCR cycles of 10 s at 95°C, 10 s at 55°C, and 10 s at 72°C; 26 PCR cycles of 10 s at 90°C, 10 s at 60°C, and 10 s at 72°C; and 15 min at ...H 2 O 2 and ... See full document
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Evaluation of the Abbott m2000 RealTime Human Immunodeficiency Virus Type 1 (HIV 1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep Cobas Amplicor, Roche Cobas AmpliPrep Cobas TaqMan HIV 1, and BioMerieux NucliSENS EasyQ HIV 1 Assays
... current NucliSENS HIV-1 technology, RealTime HIV-1 re- quires less manual preparation (38) and therefore fewer ...This assay showed overall good perfor- mance and potential for use with ... See full document
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Multicenter Comparison of Roche COBAS AMPLICOR MONITOR Version 1 5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex Version 3 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... an assay can be influenced by characteristics such as specimen throughput and volume requirements, ease of execution, in- strument work space, and costs (Table ...and NucliSens are equivalent to that of ... See full document
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Design and Evaluation of a Human Immunodeficiency Virus Type 1 RNA Assay Using Nucleic Acid Sequence Based Amplification Technology Able To Quantify Both Group M and O Viruses by Using the Long Terminal Repeat as Target
... (e.g., NucliSens HIV-1 QT assay [Or- ganon Teknika, Boxtel, The Netherlands] or Amplicor version ...direct detection of HIV-1 RNA by hybridization with labeled probes ... See full document
6
Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... HIV RNA Quantitative ...the assay controls and clinical samples were ex- tracted by centrifugation and separation using a QIAAmp col- umn and subjected to amplification with primers targeted at a ... See full document
5
Flow cytometric immunofluorescence assay for detection of antibodies to human immunodeficiency virus type 1 using insoluble precursor forms of recombinant polyproteins as carriers and antigens
... detect human immunodeficiency virus type 1 (HIV-1) infection since the tests were first licensed in 1985 ...the detection of HIV DNA and RNA by PCR and virus ... See full document
8
Pharmacologic and nonpharmacologic options for the management of HIV infection during pregnancy
... HIV RNA 1000 copies/mL, the data are insufficient to determine if MTCT rates would be further reduced by elective cesarean ...HIV RNA levels of 1000 copies/mL were reported to have MTCT rates of ... See full document
13
Development, Evaluation, and Validation of an Oligonucleotide Probe Hybridization Assay To Subtype Human Immunodeficiency Virus Type 1 Circulating Recombinant Form CRF02 AG
... hybridization assay, CRF02_AG predictions for the validation panel of the differ- ent cohorts were as follows: Coˆte d’Ivoire, ...the assay results were compared with the subtype classifications of ... See full document
6
Highly Sensitive Multiplex Assay for Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) ...screening plasma specimens for the presence of HIV-1 and HCV ... See full document
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Determination of Coreceptor Usage of Human Immunodeficiency Virus Type 1 from Patient Plasma Samples by Using a Recombinant Phenotypic Assay
... Human immunodeficiency virus type 1 (HIV-1) entry into target cells relies on a complex interaction between viral and cellular proteins, eventually leading to viral and ... See full document
9
Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
... dried plasma spots (DPSs) as a source of material for virus quantification, human immunodefi- ciency virus type 1 (HIV-1) RNA levels were quantified in matched DPS ... See full document
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Determinations of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma: Reassessment of Parameters Affecting Assay Outcome
... HIV-1 RNA levels in blood in transmission and disease ...HIV-1 RNA testing ideally be collected in tubes con- taining EDTA, processed within 6 h of collection (but up to 24 h is still ... See full document
9
HIV Load Testing with Small Samples of Whole Blood
... established plasma VL assay for the use with WB. The assay principle of the CAP/CTM in- cludes a nonspecific capture step based on silica beads and a RT-PCR and therefore does not discriminate ... See full document
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