[PDF] Top 20 Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real Time PCR
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Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real Time PCR
... of PCR had an initial denaturation step at 95°C for 15 ...Nested PCR conditions were as follows: denaturation at 95°C for 15 min, followed by 30 amplification cycles (94°C for 30 s, 55°C for 30 s, and 72°C ... See full document
5
Rapid Detection of Human Immunodeficiency Virus Type 1 Subtype E Infection by PCR
... E virus has been reported to be associated with a higher risk of heterosexual transmission than subtype B virus ...E virus grew more efficiently in Langerhans cells than subtype B ... See full document
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Performance of a Novel Human Immunodeficiency Virus (HIV) Type 1 Total Nucleic Acid Based Real Time PCR Assay Using Whole Blood and Dried Blood Spots for Diagnosis of HIV in Infants
... In order to minimize user interventions and improve sample throughput, a new assay was designed by Roche for the Cobas AmpliPrep/Cobas TaqMan (CAP/CTM) system, making use of automated sample extraction connected to ... See full document
5
Development and Assessment of a Multiplex Real Time PCR Assay for Quantification of Human Immunodeficiency Virus Type 1 DNA
... of human immunodeficiency virus type 1 (HIV-1) DNA are associated with a faster progression to AIDS, an increased risk of death, and a higher risk of HIV RNA rebound in patients on highly ... See full document
6
Real Time PCR Assay of Individual Human Immunodeficiency Virus Type 1 Variants in Coinfected Human Lymphoid Tissues
... quantitative real-time PCR by using the ABI Prism 7700 sequence detector (Applied ...Green PCR mas- ter mix (Applied Biosystems), each primer at 300 nM, and 10 l of ... See full document
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Simultaneous Detection of Multiplex Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere Based Hybridization Assay
... of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNAs and hepatitis B virus (HBV) DNA is ...biotinylated PCR products to hybridize to ... See full document
6
Single Rapid Real Time Monitored Isothermal RNA Amplification Assay for Quantification of Human Immunodeficiency Virus Type 1 Isolates from Groups M, N, and O
... a rapid drop in viral RNA levels, which remained low or even below the detection level of each assay until a virological drug failure was observed as deter- mined by increasing viral RNA ... See full document
7
Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... different real-time PCR system, which amplified a 149-bp fragment to detect ...The real- time PCR approach increased the detection of PCV2 samples by 18% over that achieved by ... See full document
5
Rapid, Point of Care Extraction of Human Immunodeficiency Virus Type 1 Proviral DNA from Whole Blood for Detection by Real Time PCR
... quantitative real-time PCR (qPCR) ther- mal cycling and detection have been performed with low-cost, handheld instruments such as the Bio-Seeq Plus instrument (Smiths Detection Group ... See full document
6
An Inexpensive and Accurate Reverse Transcription PCR–Melting Temperature Analysis Assay for Real Time Influenza Virus B Lineage Discrimination
... A, B, and C and the recently described type D ...and B are major causes of impact on public health. Influenza B is responsible for fewer cases of disease in most seasons, but a significant ... See full document
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Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... the real-time assay, the numbers of HIV copies used in the ...single real-time run to determine the intrarun variability (see also Table ...(A) Real-time HIV-1 amplification ... See full document
7
MultiCode RTx Real Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples
... of PCR. (B) Reaction ...Step 2 is allele-specific extension that relies on differ- entiation between K65R and K65K codons based on an only 1-base ...of real-time PCR datum ... See full document
5
Real Time PCR Assay for Clinical Management of Human Immunodeficiency Virus Infected Patients with Visceral Leishmaniasis
... advanced human immunodeficiency virus (HIV) infection in patients living in the Mediterranean ...and 2 to 9% of HIV-infected patients acquire this dis- ease (4, ...Recently, PCR for ... See full document
6
Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real Time PCR
... Plasma RNA detection. Viral RNA was extracted from 1 ml of plasma and ultracentrifuged for 1 h at 4°C using an HCV specimen preparation kit (Roche Diagnostics Systems). RNA was amplified by a one-step procedure using a ... See full document
5
Rapid and Sensitive Method Using Multiplex Real Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
... by virus isolation in cell culture and immunofluorescent ...transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA ...A rapid ... See full document
6
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... transcriptase PCR assay (3), has been widely used in research as well as in clinical ...the PCR, resulting in a dynamic range of 500 to 500,000 ...The real-time PCR technology already ... See full document
5
Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... subtype B, the most frequent subtype found in the European population, representing 80 to 85% of the strains isolated in ...each type (data not ...against human T-cell leukemia virus was also ... See full document
8
Rapid Preclinical Detection of Sheeppox Virus by a Real Time PCR Assay
... The Capripoxvirus (CaPV) genus of the Poxviridae family of viruses comprises sheeppox (SPPV), goatpox (GTPV), and lumpy skin disease (LSDV) viruses, which cause disease in sheep, goats, and cattle, respectively. These ... See full document
5
Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... Probe and Primer design. For performance, target RNA sequences were selected within the regions of the respective virus genomes showing maximum sequence conservation for the ability of the test to de- tect all ... See full document
6
Mechanistic differences between HIV 1 and SIV nucleocapsid proteins and cross species HIV 1 genomic RNA recognition
... of virus replication, the HIV-1 NC protein interacts with structured RNA elements present at the 5 ′ end of the viral genome: the TAR stem-loop (SL), which is at the extreme 5 ′ end of R in gRNA ... See full document
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