[PDF] Top 20 Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles
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Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles
... The only difference was that HCV RNA purified from 50 pul of HCV antibody-positive sera was first serially diluted 10-fold in normal serum, using either the phenol-chloroform extraction [r] ... See full document
5
Detection of hepatitis C virus RNA by methods using phenol chloroform or silica particles
... and silica particle methods starting with a 100-ml serum ...carrier. RNA was washed in 70% ethanol after precipitation in isopropanol, and the pellet was resuspended in 30 m l of diethylpyrocarbonate- ... See full document
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Multicenter Evaluation of the VERSANT HCV RNA Qualitative Assay for Detection of Hepatitis C Virus RNA
... Qualitative hepatitis C virus (HCV) RNA assays play a key role in both the diagnosis and monitoring of HCV ...HCV RNA assays detect viral genomes and therefore can both confirm the ... See full document
6
Hepatitis C virus detection by single round PCR specific for the terminal 3' noncoding region
... HCV RNA has usually required assay strategies that employ multiple amplification ...HCV detection are labor- intensive, time-consuming, and, in the case of nested PCR, prone to ...of detection is ... See full document
7
Detection of Anti Hepatitis C Virus Effects of Interferon and Ribavirin by a Sensitive Replicon System
... anti-HCV status or modify the sensitivity to anti-HCV agents. Thus, the robust replication of this reporter replicon may be essential to detect the anti-HCV effect with higher sensitivity and accuracy. Second, in this ... See full document
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Automated Multiplex Assay System for Simultaneous Detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Type 1 RNA
... specific particles have been developed (5, ...detecting hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunode- ficiency virus type 1 (HIV-1) has been ... See full document
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Highly Sensitive Multiplex Assay for Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... with rapid emission kinetics, known as a flasher (ortho- fluoroacridinium ester), is used to detect the internal ...the RNA target and is extended via the DNA polymerase activity of the reverse ... See full document
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The development of a GeXP based multiplex reverse transcription PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes
... a rapid and sensitive diagnosis of respiratory viral ...vitro-transcribed RNA of all RNA viruses and the plasmids containing the Adv and HBoV target ...evaluated using 126 clinical ... See full document
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Evaluation of dried blood spots as a feasible alternative to plasma for detection, quantification and genotyping of hepatitis C virus: A pilot study
... are sensitive and specific for HCV antibodies and HCV RNA and can be used as an alternative to ...effective method for HCV screening ...HCV RNA detection, quantification and ...HCV ... See full document
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Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano gold immunological amplification and silver staining method
... parallel detection of thousands of addressable elements in a single ...high sensitive visual detection based on two-probe sandwich hybridization/nanoparticle amplifi- cation have been employed, and ... See full document
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Combined immunoaffinity cDNA RNA hybridization assay for detection of hepatitis A virus in clinical specimens
... To apply cDNA-RNA hybridization methods to the detection of hepatitis A virus HAV in clinical materials, developed a two-step method in which a microtiter-based, solid-phase immunoadsorp[r] ... See full document
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Direct detection of circulating hepatitis C virus RNA using probes from the 5' untranslated region
... absent using normal human serum, horse serum, or hepatic cellular RNA from noninfected ...HCV RNA and was abolished by pretreatment with RNase ...HCV RNA PCR and 20 patients with liver ... See full document
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Detection of Marek's Disease Virus DNA in Chicken but Not in Human Plasma
... on an epidemiological study which showed an unusually high prevalence of MS in Key West, Fla. (3). Then it was postulated that seabirds were likely vectors of an environmental pathogen which could have caused this ... See full document
5
Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells
... of detection obtained after analysis of a serum and a liver sample performed using the dif- ferent primer pairs described ...ficity using different combinations of ...strand RNA templates were ... See full document
8
Rapid and Sensitive RT QuIC Detection of Human Creutzfeldt Jakob Disease Using Cerebrospinal Fluid
... and/or IQ-CSF conditions. (A) Averaged RT-QuIC kinetics for sCJD and non-CJD CSF samples. Mean ThT fluorescence for all tested CSF specimens from non-CJD ... See full document
7
The α Isoform of Protein Kinase CKI Is Responsible for Hepatitis C Virus NS5A Hyperphosphorylation
... expected, the NS5A hyperphosphorylation inhibitors H479 and A852 showed a marked reduction in p58 (p58/p56, 0.3 to 0.4) (Fig. 2, lanes 2 and 3), while the control lanes showed a p58/ p56 ratio of around 0.8. All CKI ... See full document
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Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients
... HCV RNA negative by CAM during and at the end of therapy had viral relapse after treatment ...of detection is 5 to 10 IU/ml) (21) at the end of therapy in CAM-negative cases could reliably predict relapse ... See full document
5
Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
... Center, using an X-ray wavelength of 0.688807 Å. Figure 3A–C, and 3D represent the spectra of α -Fe 2 O 3 , Fe 3 O 4 , MNPs, and the APTES-modified MNPs, ... See full document
7
Prevalence of HCV Genotypes in Various Geographical Regions
... In polymerase chain reaction, a specific portion of the HCV sequence 5’UTR was extracted and processed with restriction fragment length polymerization following the line probe reverse hybridization and finally, they were ... See full document
8
Characterization of Low- and Very-Low-Density Hepatitis C Virus RNA-Containing Particles
... Preparation of low-density fractions. Plasma from infected patients or from blood donors was separated by sequential ultracentrifugation to obtain three low-density fractions whose densities corresponded to those of ... See full document
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