[PDF] Top 20 Rapid and sensitive quantitative immunoassay for the large simian virus 40 T antigen.

... To confirm that this approach applies Replicates containing 2.5, 7.5, 12.5, and 17.5 Al of a to the quantitation of highly purified large T partiallypurified SV80 large-T-antigen fractio[r] ... See full document

... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ... See full document

... are large-T antigen, small-t antigen, and the recently identified 17kT (58), which arise from a single primary transcript as a result of differential ...and virus infection ...by ... See full document

... that T-ag could interact, albeit more weakly, with two noncanonical se- quences, both 5 ⬘ -TCGGC-3 ⬘ , present in the origin ...by 40%, whereas an identical substitution in P4 (to 5 ⬘ -GAAGC- 3 ⬘ ) ... See full document

... KATHLEEN RUNDELL Department of MicrobiologylImmunology and Cancer Center, Northwestern University Medical and Dental Schools, Chicago, Illinois 60611 Received 24 August 1983/Accepted 9 N[r] ... See full document

... To determine the distribution of various forms of T antigen on the sucrose density gradients at a quantitative level, the corresponding gel bands were excised, eluted with 0.2 ml of 60% [r] ... See full document

... tumor virus studies ...small t antigen, respectively), or dlA2433, a mutant with a misfolded carboxyl half due to a deletion of aa 587 to 589 ...carboxyl-region T-antigen functions, ... See full document

... between large T and p53 for SV40-mediated cellular transformation is firmly estab- lished (16, 23, 52, 56, 59, 73, 74), although the functional consequences of this interaction are not fully understood and ... See full document

... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ... See full document

... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document

... However, mutants which lack origin-binding activity retain the ability to transform both primary and established cells phenotypically, indicating that It has been known for some time tha[r] ... See full document

... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ... See full document

... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document

... 711-716 0022-538X/85/120711-06$02.00/0 Copyright © 1985, American Society for Microbiology Simian Virus 40 Large T Antigen Oligomers: Analysis of Electrophoresis in the Absence of Deterg[r] ... See full document

... Peptides used for immunization of mice and in characterization of antibodies Designation Amino acid sequences corresponding to Tthe of SV40 large carboxy-terminal region Lys-Thrz Hexamer[r] ... See full document

... When virus-infected cell extracts are separated on 5 to 40%0 sucrose gradients, wild-type extracts show the greatest accumulation of viral late protein in the 200S-240S fractions corresp[r] ... See full document

... 531-538 0022-538X/83/020531-08$02.00/0 Copyright © 1983, American Society for Microbiology Self-Assembly of Simian Virus 40 Large T Antigen Oligomers by Divalent Cations MATHIAS MONTENAR[r] ... See full document

... The 96,000-molecular-weight anti-T-reactive surface protein was present in SV40-transforned mouse and hamster cells, as well as in productively infected monkey cells, but was not detecte[r] ... See full document

... Preliminary data suggest that PAb11O4 can bind to D2 protein, which lacks the N-terminal 82 amino acids of large T, whereas, in blocking studies with anti-T antibodies of the PAb200 seri[r] ... See full document

... The ability of p185 to be precipitated from the in vitro mix depends on the presence of T antigen, as pretreatment of the unlabelled A16 lysate with an anti-T monoclonal antibody to remo[r] ... See full document