[PDF] Top 20 Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
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Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... HBV PCR products from these samples were cloned into a T vector and then sequenced by Sangon Biotech ...plasmid DNA were measured using a Meriton SMA6000 (Meriton, USA) and converted to copies/ml and IU/ml ... See full document
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Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... and quantification of hepatitis B virus (HBV) DNA are essential for the diagnosis and treatment of chronic HBV ...of real-time PCR assays for HBV DNA ... See full document
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Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... HBV DNA assay is sensitive, specific, and reproducible and that it accurately quantifies HBV DNA in samples from patients with chronic HBV ...infection. Quantification is linear over the full dynamic ... See full document
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Comparison of Conventional PCR with Real Time PCR and Branched DNA Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5
... both assays (HPS/CTM and CAP/CTM) rely on the same test ...and DNA polymerase activ- ities. The quantification standard represents a noninfectious RNA construct containing fragments of HCV sequences ... See full document
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Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... Hepatitis B virus (HBV) is an important cause of human chronic liver diseases and is a major public health ...in hepatitis B patients. Viral genotype detection and quantification ... See full document
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Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays
... HBV DNA levels were monitored using the Roche Molecular Systems Amplicor HBV MONITOR Assay in accordance with the instructions of the ...For PCR, a primer set which amplifies a 104-bp fragment of the ... See full document
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COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... HBV DNA is undetectable in plasma, even with the most sensitive methods, such as CAP- CTM, leaving open the question of how to detect occult-HBV carriers in important settings such as blood donations, apart from ... See full document
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Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... the PCR without further ...genotype may alternatively be obtained directly. This would require inclusion of a 72°C ex- tension step (which is otherwise used only in the set 2-2 PCR), but it ... See full document
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Comparison of Real Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy
... Switzerland) assays have better sensitivities of 200 and 300 copies/ml, respectively, and have been used for the monitor- ing of HBV patients undergoing antiviral therapy and also of liver transplant recipients ... See full document
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Application of real time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia
... Hepatitis B virus (HBV) is the leading cause of viral hepa- titis in humans ...over two billion people have evidence of previous HBV infection and 350 million have become chronic carriers of ... See full document
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Performance Characteristics and Comparison of Abbott and artus Real Time Systems for Hepatitis B Virus DNA Quantification
... these assays could be due to the difference in sample volumes: ...obtained DNA concentration. Therefore, DNA prepared from a high sample volume and resuspended in low-elution buffer enhances the ... See full document
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... of PCR. As an alternative to synthetic fragments of RNA or DNA, housekeeping genes or different pathogens are used as an internal ...the PCR assay than in vitro-transcribed RNA ... See full document
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Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments
... diagnostic PCR assays, the use of ICs is necessary to demonstrate the absence of PCR inhibition ...As PCR inhibitors are not always reliably removed from clinical speci- mens during DNA ... See full document
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Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients
... The quantification of hepatitis C virus (HCV) RNA is es- sential for the management of chronic hepatitis C therapy based on the combination of pegylated interferon (PEG-IFN) and ribavirin ... See full document
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Multilaboratory Evaluation of Real Time PCR Tests for Hepatitis B Virus DNA Quantification
... of real-time assays have mainly focused on the performance of individual tests compared to signal ampli- fication tests rather than the comparative performance of mul- tiple real-time ... See full document
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Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA
... Epstein-Barr virus (EBV)-associated ...quantitative assays for EBV based on real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification ...nested ... See full document
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Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
... LC PCR Reagents (Artus), and the COBAS AMPLICOR HBV MONITOR Test (Roche) were performed according to the instructions of the ...HBV DNA was done with the COBAS AMPLIPREP Total Nucleic Acid Isolation kit ... See full document
5
A Genotype Independent Real Time PCR Assay for Quantification of Hepatitis B Virus DNA
... Our assay has similar amplification efficiencies across all known HBV genotypes, including genotype H; and intra- and interassay variabilities were also comparable across these eight genotypes. Correlation between the ... See full document
6
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... HBV DNA quantification, including hybrid capture and branched DNA (bDNA) signal amplification (19–21, 35), assays based on real- time PCR technology (12) offer the ... See full document
9
Sensitive Enzyme Immunoassay for Hepatitis B Virus Core Related Antigens and Their Correlation to Virus Load
... Purified DNA was washed twice with 70% ethanol and resus- pended in 20 l of distilled ...of DNA solution was used for RTD-PCR, which was performed using the LightCycler System (Roche Diag- ... See full document
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