[PDF] Top 20 Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
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Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
... Epstein-Barr virus shuttle vector was recently developed to study the adeno-associated virus (AAV) site-specific integration event in chromosome ...the recombinant ... See full document
8
Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
... Rep-mediated site-specific integra- tion requires the RBS and the trs and involves multiple strand switching during novel DNA strand synthesis within a Rep- mediated complex formed between ... See full document
10
Isolation of Recombinant Adeno-Associated Virus Vector-Cellular DNA Junctions from Mouse Liver
... vector integration, the unique aspect of our study is the demonstration, by Southern blotting, of dele- tions in the ITRs and their flanking sequences in the concate- mers, which was later confirmed by DNA ... See full document
10
Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus–Adeno-Associated Virus Vector
... the site of gene ...of integration of the recombinant Ad genome and by the development of a strong cellular immune response to the genetically corrected cells that express low levels of viral ... See full document
10
Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad ...the ... See full document
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Kinetics and Frequency of Adeno-Associated Virus Site-Specific Integration into Human Chromosome 19 Monitored by Quantitative Real-Time PCR
... AAV site-specific ...highly specific for AAV ITR/AAVS1 ...AAV-2 integration into chr-19 ...the integration efficiencies of AAV ITR and/or rep ...for site specificity of ... See full document
6
Genetic Fate of Recombinant Adeno-Associated Virus Vector Genomes in Muscle
... DNA junctions from muscle tissue with very high vector copy ...ciency virus DNA integration (3). In that human immunode- ficiency virus assay, integration frequency was determined by ... See full document
10
Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... Adeno-associated virus (AAV) type 2 is a nonpathogenic, replication-defective parvovirus that is dependent on superin- fection with helper adenovirus for efficient replication ...chromosomal ... See full document
8
Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... RU486-dependent site-specific integration mediated by ...ITR/AAVS1 junctions were amplified from the genomic DNA extracted from cells subcultured for 14 days and detected with an AAVS1-derived ... See full document
14
Efficient Site-Specific Integration of Large Transgenes by an Enhanced Herpes Simplex Virus/Adeno-Associated Virus Hybrid Amplicon Vector
... the integration mechanism, nested PCR was performed to amplify the integration junc- tions, followed by sequencing ...the junctions of both flip and flop integrations, two sets of primers for the ... See full document
8
Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination
... Though site-specific integration is dependent upon either of the two large Rep proteins, the AAV ITRs are the only cis elements required for integration (34, 44, ...the virus will still ... See full document
14
Recruitment of wild-type and recombinant adeno-associated virus into adenovirus replication centers.
... simian virus 40, bovine papillomavirus type 1, human immunodeficiency virus type 1, and adenovirus, as well as inhibits cellular replication (1, 4, 21, 26, 36, ...to specific DNA sequences within the ... See full document
15
Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer
... The importance of episomal forms is increasingly recognized as the dominant factor in rAAV-mediated expression and per- sistence. In one of the earliest studies, rAAV genomes were present as approximately 8 kb ... See full document
11
Packaging of Human Chromosome 19-Specific Adeno-Associated Virus (AAV) Integration Sites in AAV Virions during AAV Wild-Type and Recombinant AAV Vector Production
... variable integration sites of AAVS1 were ...AAV site-specific integration at AAVS1 on ...packaged junctions were more rearranged than the ones derived from latently infected HeLa cells ... See full document
7
Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... This virus has the unique ability during latent infection to undergo site-specific integration of its viral DNA into the AAVS1 region in chro- mosome 19 ...the site-specific ... See full document
13
A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette
... per- formed plasmid transfection experiments (which exclude viral DNA ...the virus-associated (VA) RNA ...and integration in Mo7e clones infected with ...control virus, ... See full document
11
Adeno-associated virus vector integration junctions.
... vector integration in our system and integration by wild-type ...vector integration that we observed, two- thirds of wild-type proviruses studied in latently infected hu- man cells were found in the ... See full document
8
Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.
... Adeno-associated virus (AAV)-based vectors may have a potential advantage over the currently used retroviral vectors due to their nonpathogenicity, physical stability, and ability to infect dividing ... See full document
8
Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185
... formed affinity chromatography using an approximately 50-bp wild-type mnAAVS1, containing the trs and the RBS, or its mutant, immobilized on latex beads (Fig. 1A). The latex beads were incubated with HeLa NE, and ... See full document
12
Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... found associated with Artemis, a cellular endonuclease, and it has been experimentally demonstrated that the DNAPKcs-Arte- mis complex can cleave hairpin loops, flaps, and ...during integration and/or after ... See full document
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