[PDF] Top 20 Rep-PCR-based typing as a tool for tracking of MRSA infection origin
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Rep-PCR-based typing as a tool for tracking of MRSA infection origin
... of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci ...the MRSA and Staphylococcus ... See full document
6
Validation of use of whole cell repetitive extragenic palindromic sequence based PCR (REP PCR) for typing strains belonging to the Acinetobacter calcoaceticus Acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak
... Acinetobacter infection linked to ventilatory equip- ment have also highlighted the need for careful decontamina- tion of reusable tubing and pressure monitors (9, 24, ... See full document
10
Efficacy of Pulsed Field Gel Electrophoresis and Repetitive Element Sequence Based PCR in Typing of Salmonella Isolates from Assam, India
... epidemiological tool (3). Strain typing is most effective as an aid to outbreak investigation when it is applied to small sets of isolates that are epidemiologically related ...molecular typing ... See full document
6
Molecular Typing of Methicillin Resistant Staphylococcus aureus: Can PCR Replace Pulsed Field Gel Electrophoresis?
... molecular typing of methi- cillin-resistant Staphylococcus aureus ...multiplex PCR-based method with published primers and compared the results with those obtained by ...multiplex PCR. Both ... See full document
6
Microbial DNA Typing by Automated Repetitive Sequence Based PCR
... different rep-PCR fingerprint pat- terns, and the patterns were virtually indistinguishable within each of the five isolates ...manual rep-PCR and analysis with the BioNumerics software as a ... See full document
9
Methicillin Resistant Staphylococcus aureus (MRSA) Detection: Comparison of Two Molecular Methods (IDI MRSA PCR Assay and GenoType MRSA Direct PCR Assay) with Three Selective MRSA Agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for Use with Infection Control Swabs
... time PCR-based IDI-MRSA assay (Infecto Diagnostics, ...GenoType MRSA Direct (Hain Lifescience, Nehren, Germany) ...of MRSA was attempted by inoculation of nonselec- tive blood agar ... See full document
5
Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed Field Gel Electrophoresis for Clonal Characterization of Methicillin Resistant Staphylococcus aureus
... Therefore, MRSA control is a cornerstone of any infection control ...ever, typing methods have also become an indispensable tool for distinguishing clonal nosocomial clusters from unrelated ... See full document
7
Multicenter Evaluation of Epidemiological Typing of Methicillin Resistant Staphylococcus aureus Strains by Repetitive Element PCR Analysis
... The MRSA strains were distributed by the coordinating center to the participat- ing centers labeled with code ...isolate origin or PFGE type using one or more rep-PCR typing ...Each ... See full document
7
Risk factors associated with community-acquired CTX-M producing Klebsiellapneumoniae typing by Rep-PCR in Sanandaj, Iran
... positive. Based on Rep-PCR, 31 genotypes were identified among 37 Samples of CTX-M positive ...protocols based on the antibiogram. The results of Rep-PCR experiment refute the ... See full document
7
Molecular Genotyping of Staphylococcus aureus Strains: Comparison of Repetitive Element Sequence Based PCR with Various Typing Methods and Isolation of a Novel Epidemicity Marker
... is based on the presence of homologues of Mycoplasma pneumoniae repeat-like elements in ...of rep-PCR typing with two sets of well-defined collections of Staphylococcus aureus ...strains. ... See full document
8
Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay
... multiplex PCR assay did not succeed in amplifying all target ent genes. Several amplicons were not represented; and the yields of seo, sen, seh, and sej were too low to be detected by microarray hybridization ... See full document
10
Improving Early Sepsis Identification on Inpatient Units
... the tool for assessing patients fall risks will be ...assessment tool (at time of admission); 2) orient the team to the assessment tool’s risk-level score ...assessment tool will serve as a guide, ... See full document
30
Discriminatory Power and Reproducibility of Novel DNA Typing Methods for Mycobacterium tuberculosis Complex Strains
... FAFLP typing has been applied successfully to various or- ganisms, such as Clostridium, Bacillus, Acinetobacter, Pseudo- monas, and Escherichia (5, ...the PCR amplification (60), or when other restriction ... See full document
11
<p>Ceftobiprole for Treatment of MRSA Blood Stream Infection: A Case Series</p>
... (vancomycin MIC < 0.5) on the second day of fever and persisted for three days, which was judged to be related to CLABSI and exit site infection. The central line was removed on the same day of obtaining the ... See full document
6
Evaluation of Ribotyping as a Tool for Molecular Typing of Yersinia pseudotuberculosis Strains of Worldwide Origin
... Comparison of the ribotypes of Y. pestis and Y. pseudotuber- culosis. Since Y. pestis was shown to be a clone recently emerged from Y. pseudotuberculosis (2), we wondered whether the two species displayed different ... See full document
6
Comparison of DNA Sequencing of the Protein A Gene Polymorphic Region with Other Molecular Typing Techniques for Typing Two Epidemiologically Diverse Collections of Methicillin ResistantStaphylococcus aureus
... spaA typing might not be able to differentiate ...spaA typing (clone I::NH::W), with nothing in common with clone III::B::A, displayed the unrelated and spe- cific spaA type YHFGFMBQBLO, whereas the ... See full document
9
Genotypic diversity among multidrug resistant Pseudomonas aeruginosa and Acinetobacter species at Mulago Hospital in Kampala, Uganda
... adequate infection control and proper ...minimal infection control practices as evidenced by low standards of hand hygiene among health care workers ... See full document
10
<p>A Combination of circRNAs as a Diagnostic Tool for Discrimination of Papillary Thyroid Cancer</p>
... Patients and Methods: The distinctive expression pro fi le of serum circRNAs was deter- mined by individual quantitative real-time PCR (qRT-PCR) in two independent cohorts of 113 PTC patients, 80 thyroid ... See full document
8
Predominance and Emergence of Clones of Hospital Acquired Methicillin Resistant Staphylococcus aureus in Malaysia
... predominant MRSA clones in Europe, Australia, and New Zealand [40]) in Singapore (18) and later in Malaysia strengthens the case for the epidemic nature of this clone and also suggests that it may easily spread to ... See full document
6
Analysis of Typing Methods for Epidemiological Surveillance of both Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Strains
... the MRSA subset, improved the predictive power of each single technique for determining the SCCmec type (W ⫽ ...for MRSA strains, this value was slightly lower (W ⫽ ...for MRSA, the PFGE-SCCmec ... See full document
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