[PDF] Top 20 In Vitro RNA Replication Directed by Replicase Complexes Isolated from the Subgenomic Replicon Cells of Hepatitis C Virus

... ds RNA production and vice ...ss RNA was further chased into ds RNA and RNA species larger than the monomeric replicon RNA, indicating that the ss RNA could serve as a ... See full document

... additional RNA is added to the in vitro reac- tion mixture, the template corresponds to the endogenous rep- licon ...radiolabeled RNA product was observed that comigrated with the originally ... See full document

... bicistronic subgenomic replicon (19), was investigated by immunofluores- cence microscopy (IF) to determine the subcellular distribu- tion of the viral nonstructural ...of cells were performed as ... See full document

... of hepatitis C virus (HCV) through two independent regions of NS5B, amino acids 208 to 214 and 500 to ...the RNA-dependent RNA polymerase activity of NS5B in vitro, suggesting ... See full document

... single-plus-strand virus RNA genome ...␤ replicase has a specific template affinity to the viral genome, thus allowing specific amplification of the Q ␤ ...single-stranded RNA viruses ... See full document

... avid RNA aptamers consisting of 2=-hydroxyl- or 2=-fluoropyrimidines against hepatitis C virus (HCV) NS5B replicase, an enzyme that is essential for HCV ...impede ... See full document

... viral RNA replication machinery in human ...HCV replication due to the lack of an efficient cell culture ...viral RNA replicon provides a suitable model to test our hypothesis (2, 12, ... See full document

... nomic replicon [H/SG-Neo(L+I)] [22] were provided by ...HCV-N subgenomic replicon was provided by ...JFH-1 replicon, the full length (fl)JFH-1 replicon, and the genomic JFH-1 viral ... See full document

... nontranslated RNA (3 ⴕ NTR) signals required for replication of subgenomic hepatitis C virus (HCV) ...HCV-N replicon that induces the expression of secreted alkaline ... See full document

... isolates from different patient sera for their biological properties in trans- fected ...clones from patients taken before and after therapy with IFN and ribavirin can now be isolated and tested for ... See full document

... isolation, RNA synthesis, and transfections. Total cellular RNA was harvested from cells by using TRIZOL ...(Invitrogen). RNA was transcribed in vitro from ScaI-linearized ... See full document

... O cells expressing shRNA against DDX3 (DDX3i#3) as well as in O cells transduced with a control lentivirus vector (Con) are ...HCV RNA was monitored by real-time LightCycler PCR ...HCV RNA. ... See full document

... T7 RNA polymerase activity during in vitro ...the RNA bands are the quantification values, in µg/µl. C) : IRES interaction assay in Huh ...7.5 cells. Replication rate 4h after ... See full document

... of replication signals in the NTRs consisted of analyzing the intergenotypic replicase chimeras in the context of NTRs from different ...intergenotypic replicase chimeras ...derived ... See full document

... Huh-7 cells were transfected with expression plasmids for wild-type GBF1 or GBF1-M832L, a BFA-resistant mutant of GBF1 ...(45). Cells were infected in the presence of BFA, fixed at 24 h ...inhibits ... See full document

... resistant replicon did not restore the full range of resistance when engineered back into a wild-type ...RS2 cells were approximately 10 times more resistant than the GS5 cells, while the I423V ... See full document

... in vitro radio- labeled replication products were eluted from an agarose gel and used as a probe for hybridization in a Northern blot which included the following RNAs ...in vitro-transcribed ... See full document

... house virus protein A (25, ...cation complexes of other positive-strand RNA viruses occurs by interactions with other viral ...mouse hepatitis virus RdRp is not mem- brane associated ... See full document

... donor cells and 1E5 recipient cells was subjected to double selection for RNA transfer, suggesting that replication-competent HCV RNA can indeed be transmitted in a virion-independent ... See full document

... B replicon for our studies as it is a commonly used HCV repli- con, which we previously used to mathematically model sub- genomic HCV replication from transfection to steady state (6); however, ... See full document