[PDF] Top 20 Replication Studies Using Genotype 1a Subgenomic Hepatitis C Virus Replicons

... for hepatitis C virus (HCV), in which the nonstructural proteins stably replicate subgenomic viral RNA in Huh7 cells, were ...of using these replicon systems to advance drug discovery ... See full document

... HCV replication and infection was evaluated by designing and constructing siRNAs against different sites of HCV structural genes having genotype1a (Core, E1 and ...whole virus of 1a genotype ... See full document

... [7]. Studies on HCV using ASOs have utilized antisense phos- phorothioate oligonucleotides (S-ODN) that were designed as complementary to sequences present in the 5' non coding region (5'-NCR) of IRES of ... See full document

... the replicons MA/ W208A, K209A, S210A, K211A, K212A, C213A, and P214A, each individual amino acid residue was changed to ...mutant replicons were transfected into Huh7-DMB cells, which were selected with ... See full document

... on replication, as a polymerase-defective RNA was still capable of ...stable replication efficiency (colony-forming activity) over several weeks, while Lohmann et ...transient replication at 24 h ... See full document

... HCV replication remains a ...between replicons with different adaptive mu- tations in NS5A ...example, replicons with S2197C, S2197P, or S2204I expressed minimal or no p58 as assessed by ... See full document

... RNA replication kinetics in cells transfected with modified genomes in which the envelope-coding regions had been deleted (to prevent any confounding influence from infectious virus pro- duction and ... See full document

... a genotype 1a HCV ...proteins C, prM, and E (YF nucleotides [nt] 161 through 2452) was replaced by the region encoding the HCV structural proteins C, E1, and E2, as well as p7 (HCV nt 342 ... See full document

... house virus RC ...the virus, as in the cases of other similar ...cell-free replication assay needed to be further ...cell-free replication system using HCV RC rather than NS5B alone is ... See full document

... drugs that separate the antiviral and immunomodulatory ef- fects of ribavirin likely will resolve this mechanistic uncertainty. In this study, we also observed antiviral activity with IFN-␣ and IFN-␥ but not with ... See full document

... ulation. Compared with the current study, these three studies had a higher population of HCV-1a (44.5% in Jensen et al.’s study; 0.5% in our study), a higher propor- tion of patients with viral loads ... See full document

... the replication of animal viruses like HIV-I (Human immunodeficiency virus), flock house virus (FVH) and poliovirus ...viral replication by targeting HCV genotype 1a and 1b ... See full document

... simplex virus, and vaccinia virus (8, ...HIV replication has been the subject of intensive studies that have led to the realization that CypA in target cells can regulate viral ... See full document

... containing replicons with R393A, F438A, and W501A muta- tions to form colonies indicates that a helicase which can hy- drolyze ATP, bind nucleic acids, and unwind DNA is not suf- ficient to support HCV ... See full document

... pressed replication (56, ...H77S.2 virus during persistent infection of chimpanzee ...the genotype 1b Con1 strain of HCV (PDB entry 3FQQ) ...calculated using the jCE/jFATCAT Structure ... See full document

... singular replication of HCV RNA in Huh-7 cells can also be attributed to another host factor, the liver-specific microRNA 122 ...HCV replication in the host cell and has been suggested to contribute to the ... See full document

... H77-derived subgenomic replicons, the inclusion of the neo gene or the EMCV IRES generally decreased the ability of RNAs to initiate ...of replication efficiencies H/⌬E1-p7 (L⫹I) ⬎ H/SG-5⬘HE (L⫹I) ⬎ ... See full document

... HCV genotype-1b clone B replicon for our studies as it is a commonly used HCV repli- con, which we previously used to mathematically model sub- genomic HCV replication from transfection to steady ... See full document

... Hepatitis C virus (HCV) was first identified as a causative agent of posttransfusion hepatitis in 1989 ...The virus is considered hepatotropic and is known to cause liver diseases such ... See full document

... HCV replicons and HCV nonstructural proteins in this study is distinct from the NS5A-induced ER overload ...and replication activities in the ER lead to this apparent ER ...Recent studies have ... See full document