[PDF] Top 20 Residues of VP26 of Herpes Simplex Virus Type 1 That Are Required for Its Interaction with Capsids
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Residues of VP26 of Herpes Simplex Virus Type 1 That Are Required for Its Interaction with Capsids
... initial interaction between VP5 and VP26 probably occurs in the cytoplasm, and this complex together with the scaffold protein translocates to the nucleus, where there is accumulation of VP26 at the ... See full document
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Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
... the capsids from all the UL25 mutants, including those that failed to show any UL25 capsid binding ...bind capsids. In studies with a UL17 null virus, it was found that the B capsids produced ... See full document
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Surface lysine and tyrosine residues are required for interaction of the major herpes simplex virus type 1 DNA-binding protein with single-stranded DNA.
... 11 0022-538X/92/116273-07$02.00/0 Copyright © 1992, American Society for Microbiology Surface Lysine and Tyrosine Residues Are Required for Interaction of the Major Herpes Simplex Virus [r] ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... mutant capsids, Western blot analysis was carried ...the interaction of UL17 or UL25 with B capsids was not dependent on the presence of UL6 and, sim- ilarly, neither UL25 nor UL17 was ... See full document
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Two regions of the herpes simplex virus type 1 UL42 protein are required for its functional interaction with the viral DNA polymerase.
... Although a UL42 mutant RNA encoding a product lacking the 28 C-terminal residues resulted in wildtype levels of pol stimulation, the failure of large aminoterminal N-terminal and C-termi[r] ... See full document
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The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-Sm antiserum, and the C terminus appears to be required for this interaction.
... The interaction was specific in that two other HSV-1 regulatory proteins, ICP4 and ICP0, that were not found to colocalize with snRNPs (37, 49) also did not immunoprecipitate with anti-Sm antisera ...was ... See full document
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The Pattern of Tegument-Capsid Interaction in the Herpes Simplex Virus Type 1 Virion Is Not Influenced by the Small Hexon-Associated Protein VP26
... intact herpes simplex virus type 1 (HSV-1) virions had revealed that the icosahedrally symmetrical interaction between the tegument and capsid involves the pentons but not ... See full document
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Identification of a minimal hydrophobic domain in the herpes simplex virus type 1 scaffolding protein which is required for interaction with the major capsid protein.
... S-labeled herpes simplex virus type 1-infected cell lysates containing ...The interaction between GST/CT and VP5 was determined by coprecipitation of the two proteins with ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... wild-type virus and a virus with a mutant version of cleav- age and packaging gene UL6, capsids localize exclusively to replication compartments, cells infected with the UL32 mutant exhibit an ... See full document
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The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids
... of virus binding, the cells were washed three times in fresh medium containing cycloheximide and either fixed in 4% paraformaldehyde in PBS or cultured in the presence of cycloheximide at ...at 1, 2 and 4 h ... See full document
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Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... DNA-containing capsids, whereas cells infected with a UL12-null mutant virus only produced SN-resistant nuclear DNA-containing capsids, with impaired SN-sensitive DNA- containing capsids in ... See full document
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Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... sids. Nuclear lysates were made from cells infected for 16 h with ⌬305 or vRR1072, digested with DNase, and fractionated on 15 to 45% sucrose gradients. To test for viral genomes, DNA was purified from aliquots of ... See full document
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Analysis of a Charge Cluster Mutation of Herpes Simplex Virus Type 1 UL34 and Its Extragenic Suppressor Suggests a Novel Interaction between pUL34 and pUL31 That Is Necessary for Membrane Curvature around Capsids
... (i) Interaction between the previously mapped interaction/targeting domains of pUL34 and pUL31 mediates targeting to the nuclear mem- ...stage, interaction between the pUL34 N domain and the pUL31 C ... See full document
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Herpes Simplex Virus 1 Small Capsomere-Interacting Protein VP26 Regulates Nucleocapsid Maturation
... HSV-1 capsids and the decrease in UL25 incorporation into capsids mediated by the VP26 null mutation may lead to an imbalance between capsid structural stability and the high pressure due to ... See full document
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The C-terminal 25 amino acids of the protease and its substrate ICP35 of herpes simplex virus type 1 are involved in the formation of sealed capsids.
... Plasmids. Plasmid pM307L encodes the HSV-1 protease with a Met-to-Leu change at residue 307, which corresponds to the initiation codon of ICP35 (9, 19, 20). Therefore, this mutation eliminates the synthesis of ... See full document
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Function of the Herpes Simplex Virus 1 Small Capsid Protein VP26 Is Regulated by Phosphorylation at a Specific Site
... of VP26 Thr- 111 regulates the subcellular localization of both it and its binding partner, VP5, in HSV-1-infected ...⌬ VP26/EGFP), YK492(VP26T111A/EGFP), YK493(VP26⌬/TA-repair/EGFP), ... See full document
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Interaction between Herpes Simplex Virus Type 1 IE63 Protein and Cellular Protein p32
... of herpes simplex type 1 (HSV-1) lytic infection is the 63-kDa nuclear phosphoprotein IE63 (also known as ...is required for the switch from early to late virus gene ... See full document
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Separate functional domains of the herpes simplex virus type 1 protease: evidence for cleavage inside capsids.
... A247S virus formed slightly smaller plaques than wt virus on BMS-S129C cells (results not ...A247S virus on BMS-S129C cells. The yield of wt virus on BMS- S129C cells was comparable to those ... See full document
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Tryptophan Residues in the Portal Protein of Herpes Simplex Virus 1 Critical to the Interaction with Scaffold Proteins and Incorporation of the Portal into Capsids
... fold-portal interaction, whereas in infected cells other func- tions compensate for the mutation’s effects on the ...bling capsids that might compensate for low affinity between mutant portal and scaffold ... See full document
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Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and localization of VP26.
... wild- type capsids also contain one or more minor proteins coded for by genes that were not represented in the baculovirus systems that we ...protein required for DNA packaging ... See full document
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