[PDF] Top 20 Use of PCR Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene Based Identification of Nocardia Species
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Use of PCR Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene Based Identification of Nocardia Species
... min, PCR products were purified with the EZNA gel extraction kit (EAZY Nucleic Acid Purification; Omega Bio-Tek, Vaulx-en- Velin, ...For hsp65 gene sequencing, the amplification protocol was ... See full document
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Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S 23S rRNA Gene Internal Transcribed Spacer PCR Restriction Analysis
... MCC species and differentiation of MCC species from other species of rapidly growing mycobacteria (RGM) and from one another is ...method, based on the variable 16S-23S rRNA gene ... See full document
7
Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex PCR Based Restriction Analysis and Direct Sequencing
... culture-based identification scheme, which takes 4 to 6 weeks or longer for the identification of slowly growing mycobacteria, PCR and PCR-linked methods have been widely used to ... See full document
8
Phylogenetic Analysis and PCR Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences
... Campylobacter species and reference strains of Arcobacter butzleri and Helicobacter pylori was studied based on partial 593-bp groEL gene ...tree based on the groEL gene was similar to ... See full document
9
Clinical Application of PCR Restriction Enzyme Pattern Analysis for Rapid Identification of Aerobic Actinomycete Isolates
... PRA database was developed primarily for clinically significant nonpig- mented rapidly growing mycobacteria, nocardiae, and other clinically significant aerobic actinomycetes (9–11, ...the database for ... See full document
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Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene
... HSP gene PCR method is unable to amplify some isolates of ...HSP gene area of the genome precludes comparison of the region with entries in sequence ...HSP gene REA appears to correlate well, ... See full document
7
Molecular Typing of Nocardia Species
... Conventional identification of Nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic ... See full document
8
Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... the hsp65 locus among different species of myco- bacteria, Telenti et ...for species identification based on analysis of polymorphisms of restriction digests of that ... See full document
6
Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... rpoB gene (Table 1). The results showed that a conserved rpoB gene present in all mycobacteria and in some other bacteria, such as Nocardia and Rhodococcus species, was ...sequently, ... See full document
6
Molecular epidemiology of indigenous isolates of Mycobacterium tuberculosis
... amplification based diagnostic tests are expensive, have limited sensitivity, are usually restricted to the detection of ...the use of genotypic tests to confirm smear positive samples, so that they cannot ... See full document
9
Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR Restriction Enzyme Analysis of hsp65
... to PCR-restriction analysis (PRA) of a 439-bp fragment of the hsp65 gene with HaeIII and ...new pattern for Mycobacterium scrofulaceum, ...new pattern for ...new ... See full document
6
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... biochemical identification tests and the Accuprobe tests and DT1-DT6 PCR (7) for the ...for PCR. PRA based on the amplification of a 439-bp fragment of the hsp65 gene was ... See full document
5
Identification of Streptococci to Species Level by Sequencing the Gene Encoding the Manganese Dependent Superoxide Dismutase
... a PCR assay based on the use of degenerate primers in order to characterize an internal fragment (sodA int ) representing approximately 85% of the genes encoding the manganese-dependent superox- ide ... See full document
7
Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... dnaJ gene was amplified by PCR with a Staphylococcus- specific pair of primers, the specificities of which have been examined previously ...coccus species and subspecies tested in this study could be ... See full document
5
Identification of various medically important Candida species in clinical specimens by PCR restriction enzyme analysis
... by PCR technology provides a more rapid and sensitive approach to the diagnosis of disseminated can- ...the use of PCR. Detec- tion of Candida DNA has been obtained by PCR amplification of ... See full document
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Diversity of Staphylococcus Species Strains Based on Partial kat (Catalase) Gene Sequences and Design of a PCR Restriction Fragment Length Polymorphism Assay for Identification and Differentiation of Coagulase Positive Species (S aureus, S delphini, S hyicus, S intermedius, S pseudintermedius, and S schleiferi subsp coagulans)
... cal species and was well supported for all the strains analyzed during this ...staphylococcal species were divided into five well-supported clusters: the ...epidermidis based on a comparison of ... See full document
10
Direct Identification of Mycobacteria in Primary Liquid Detection Media by Partial Sequencing of the 65 Kilodalton Heat Shock Protein Gene
... the identification of 20 isolates at a cost to our laboratory of ...direct identification (39 tests ⫻ $19) of ...complex identification was confirmed by the Accuprobe ...confirmed ... See full document
7
Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
... The allele frequencies and genotype distributions for FCN3+1637delC mutation were determined in the case and control subjects (Table 2). According to the data 94.4% of diabetic and 91.9% of normal samples did not show ... See full document
7
Identification of murine helicobacters by PCR and restriction enzyme analyses
... for PCR amplification with the Helicobacter genus-specific ...bacterial species that are infrequently found in the intestinal tracts of mice, ...the PCR assay was determined by ampli- fying 10-fold ... See full document
5
Use of the omp50 Gene for Identification of Campylobacter Species by PCR
... Porins are pore-forming proteins allowing exchanges of hy- drophilic compounds across the outer membranes of gram- negative bacteria. They are involved in adaptation of many bacteria to their environment (5, 8, 14). ... See full document
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