[PDF] Top 20 Role of Cellular Phosphatase cdc25C in Herpes Simplex Virus 1 Replication

... from cellular lysates were immunoblotted for cyclin B1 and ...HSV-1(F)-infected cdc25C ⫹/⫹ MEFs (Fig. 1A, lanes 1, 3, 5, and ...in cdc25C ⫺/⫺ cells but at a rate similar to that of ... See full document

... possible role of DAI in HSV-1 replication, DAI-depleted HepG2 cells were produced by transduction with lentiviral vectors expressing shRNAs for ...of 1 or 3 for 24 h and measured the amounts ... See full document

... key cellular transcription factor that plays a central role in cellular responses to a broad range of stress factors through its regulation of a variety of cellular pathways, such as ... See full document

... reported role of RPA in uninfected cells is to regulate homologous ...Progeny virus was collected at 24 h postinfection, and viral titers were determined on Vero ... See full document

... viral replication cycle is largely ...major role during productive ...pseudorabies virus (PRV) and HSV-1 ...a role in HSV- induced inhibition of ATP production and/or mitochondrial mo- ... See full document

... in virus yield (10- and 20-fold, respec- tively); however, analysis of the block in the virus life cycle revealed that MLH1 acts at a step prior to that of ...this role is indepen- dent of ... See full document

... for 1 h at 45,000 rpm in an ...KCl, 1 mM EDTA, 10 mM b -mercaptoethanol, ...a 1-ml Mono Q anion-exchange FPLC column (Pharma- cia) at a rate of 1 ml/min and eluted with a 50 mM to ... See full document

... with 1% formaldehyde for 10 ...buffer 1 (50 mM HEPES-KOH [pH 7.5], 140 mM NaCl, 1 mM EDTA, 10% glycerol, ...NaCl, 1 mM EDTA, ...NaCl, 1 mM EDTA, ...of 1%, and the samples were ... See full document

... The cellular prion protein (PrP) often plays a cytoprotective role by regulating autophagy in response to cell ...restricts replication of several viruses, but whether this activity is related to an ... See full document

... the virus life cycle and pathogenesis ...ant virus using BAC recombineering technology. The recombinant virus with the NES mutation in VP19C did not impair virus replication, and viral ... See full document

... (C) Virus production was measured after infection of wt and double-KO cells with ...The virus titer was determined at various times postinfection in two independent ... See full document

... Viruses have evolved distinct means to counteract the hurdles they encounter during infection (47). It is possible that viruses unable to counteract SAMHD1 do not require infection of my- eloid cells as a crucial step ... See full document

... Multiple cellular chromatin-remodeling factors and histone- modifying enzymes are recruited to HSV-1 replication compart- ments (RCs) (13), which are nuclear domains where viral DNA ... See full document

... Herpes simplex virus (HSV) exhibits altered gene regulation in neuronal compared to nonneuronal ...of replication (oriS and oriL) is a critical step in the upregulation of transcriptional ... See full document

... the role of ICP34.5 in disruption of autophagy through beclin1 from its role in inhibition of the IFN response through ...viral replication, or ... See full document

... secreted virus for wild-type HSV-1 as well as an ICP0-null mutant, without affecting viral gene expression, suggesting a possible role for this protein in viral as- sembly in the cytoplasm or ... See full document

... into 1 ml of TBS supplemented with leupeptin, pepstatin, and ...at 1:10,000, 5E10 was used at 1:500, and PG-M3 was used at ...a 1:10,000 dilution for 1 to 2 h, washed, and developed ... See full document

... FIG 1 Role of PKD and its modulators in vesicular cargo transport at the TGN. Step 1. Cytoplasmic PKD is recruited to the TGN by DAG and Arf1. Step 2. DAG activates nPKCs that in turn activate PKD by ... See full document

... Herpes simplex virus 1 (HSV-1) causes one of the most prevalent herpesviral infections in humans and is the leading etiological agent of viral encephalitis and eye ...the ... See full document

... Fig. 1. ICP8 was detected in nuclear replication foci upon coexpression of the heterotri- meric helicase-primase complex with both AAV constructs ...(Fig. 1, panels A and ...(Fig. 1, panels ... See full document