[PDF] Top 20 SAMHD1 Limits HIV-1 Antigen Presentation by Monocyte-Derived Dendritic Cells

... exogenous antigen presen- tation. In the absence of Vpx, antigen levels are low, and although CTL stimulation can occur through exogenous presentation of antigens, this process is less efficient than ... See full document

... encounter HIV-1-infected ...by SAMHD1. SAMHD1 levels are not modified in MDDCs cocultivated with infected ...degrades SAMHD1, or silencing of SAMHD1 with siRNAs signif- icantly ... See full document

... Nevertheless, without excluding a possible role for sCPXV012 blocking endosomal TAP, the pronounced inhibition of antigen proteolysis by sCPXV012 observed in this study smothers antigen cross- ... See full document

... Clinical vaccine WT1-loaded mo-DCs from AML patients express and present RHAMM We investigated RHAMM expression and presentation by thawed AML patients’ WT1 EP DCs used in our clinical vaccination trials. Similar ... See full document

... of cells remained ...infected cells is usually efficiently inhibited by HSV-1, its contribution to the decrease in cell viability should not be discounted, as the inhibitory effect is highly species ... See full document

... of antigen presentation and co-stimulatory molecules and for their efficacy to induce T cell-mediated anti-tumor re- ...T cells, which were then cultured with tumor cells to assess their ... See full document

... the antigen-presenting capacity and tumor cell antigen- specific CTL responses of tumor cell total RNA-electroporated human monocyte-derived DCs in ...of antigen presentation and ... See full document

... Optimal antigen presentation and subsequent T-cell re- sponses require DC ...or 1 or 10 ␮ g of LPS/ml, and the cell surface expression of CD80/86, CD40, and MHC-II was assessed by flow ... See full document

... on antigen presentation of peptides and strong costimu- lation from professional ...T cells (32, 38, ...peptide presentation and upregulate the expression of costimulatory molecules and ... See full document

... of antigen uptake, processing and presentation by monocyte- derived DC prepared in three days (3d mDC) compared with conventional DC prepared in seven days (7d mDC), which represent the most ... See full document

... In conclusion, this study further supports SWATH-MS as a robust technology for the quantitative study of proteins involved in cellular processes, such as is demonstrated here for pathogenic stimulation of moDCs. The data ... See full document

... impairing HIV tran- scription in CD4 ⫹ T cells through inhibition of CDK2 and CDK9 (66, ...of HIV-1 replication in primary myeloid ...control HIV-1 replication indirectly through ... See full document

... investigated HIV-2 replication in primary CD4+ T lymphocytes, both activated and non-activated, as well as in ...productive HIV-2 infection, using the reference HIV-2 ROD strain, the proviral clone ... See full document

... by HIV-1 in DCs, emphasizing the role of network interac- tions rather than individual connections among signaling components, transcription factors, miRs and their pre- dicted targets in this ...in ... See full document

... and monocyte/macrophage lineage cells, during HIV infection or upon exposure to recombinant HIV pro- teins ...6 HIV gp120-induced late STAT1 and STAT2 activation is not responsible for ... See full document

... endogenous antigen presentation by DCs to allogeneic T cells and exogenous OVA peptide pre- sentation to DO11 and primary CD4 ⫹ T cells are impaired during chronic ethanol consumption in ... See full document

... dendritiform cells of an increased accessory potency showing reduced Mel ...into monocyte derived dendritic cells (m-DC). After 1-4 days of incubation , the cells will ... See full document

... R5 HIV-1 isolates for 2 h and then thoroughly washed to remove unbound free viral ...particles. HIV-1-loaded MoDCs were incubated with or without autologous PHA-activated or nonactivated CD4 T ... See full document

... Jurkat cells with 2-fold serial dilutions of virus ...infected cells was determined by flow cytometry follow- ing intracellular staining of Gag with KC57 ... See full document

... to HIV-1 infection than fresh Mo (14, ...with HIV-pulsed imDC demonstrated that for productive infection of Mo a 10-fold-lower viral input was ...the HIV infection to Mo and MDM but also to ... See full document